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2019 Vol.32, Issue 5 Preview Page

Research Article

Inhibitory Effects of Hot Water Extract of Beta vulgaris L. on Triglyceride Biosynthesis Using Rhodosporidium toruloides

Rhodosporidium toruloides를 활용한 비트 열수추출물의 중성지방 억제효과

Ju-Won Kang1
,
Ya-Fei Zhao1
,
Byung-Yong Ahn2*
강 주원1
,
Zhao Ya-Fei1
,
안 병용2*
1The Master’s Course Student, Department of Oriental Medicine Resources, Chonbuk National University, Iksan 54596, Korea
2Professor, Department of Oriental Medicine Resources, Chonbuk National University, Iksan 54596, Korea
1전북대학교 한약자원학과, 대학원생
2전북대학교 한약자원학과, 교수
* Corresponding Author
31 October 2019. pp. 415-422
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Abstract

Rhodosporidium toruloides has been known as an in vitro model of fat metabolism. The purpose of this study is to identify the triglyceride inhibitory effects of hot water extracts from beetroot using R. toruloides. The triglyceride content of oleaginous yeast cultured from YPD culture medium were regulated by treatment of beet root hot water and ethanol extract, respectively. In addition, the number of cells in the oleaginous yeast was 10.48, 8.46, 12.40, 12.80 and 8.24×103 cell/mL. The treatment of hot water extract of beet root increased total lipid content of oleaginous yeast in dose dependently. Moreover, the triglyceride content of oleaginous yeast was decreased by hot water extract of beet root extract, respectively. The fat droplet in the oleaginous yeast decreased according to the concentration of hot water extracts from beetroot. The ratio of increase in the number of cells in the oleaginous yeast were increased dose-dependently by treatment of hot water extract from beetroot compared with control group. The free fatty acid and total carotenoid contents were increased concentration-dependently by treatment of hot water extracts from beetroot. These study results indicate that hot water extracts from beetroot has a triglyceride inhibitory effects.

Keywords
Beetroot
Carotenoid content
Free fatty acid content
Triglyceride content
Total lipid content
References
1
Boo, H.O., S.J. Hwang, C.S. Bae, S.H. Park and W.S. Song. 2011. Antioxidant activity according to each kind of natural plant pigments. Korean J. Plant Res. 24(1):105-112 (in Korean).
10.7732/kjpr.2011.24.1.105
2
Chea, S.G., G.S. Gang, Y.D. Ryu, S.J. Ma, G.W. Bang, M.H. Oh and S.H. Oh. 2007. Standard Food Analysis. Jigu Publisher, Gyeonggi-do, Korea. pp. 336-350 (in Korean).
3
Chen, H. C. and R. V. Farese Jr. 2005. Inhibition of triglyceride synthesis as a treatment strategy for obesity: lessons from DGAT1-deficient mice. Arterioscler Throm Vas. 25(3):482- 486.
10.1161/01.ATV.0000151874.81059.ad15569818
4
Kim, B.S., Y.J. Oh, N.H. Kim, H.J. Song, M.J. Kim, S.B. KO and J.W. Jo. 2010. Manufacture for make use of red-beet functional a drink. Presented at Spring Conference of Korean Academia- Industrial Cooperation Society. Cheonan, Korea. pp. 736-737.
5
Kim, H.K., J.H. Chun and S.J. Kim. 2015. Method development and analysis of carotenoid compositions in various tomatoes. Korean J. Environ Agric. 34(3):196-203 (in Korean).
10.5338/KJEA.2015.34.3.23
6
Kim, S.W., N.S. Kim, M.J. Oh, H.R. Kim, M.S. Kim, D.Y. Lee, S.H. Yoon, M.Y. Jung, H.J. Kim, C.H. Lee and C.H. Oh. 2017. Anti-obesity effects of fermented soybean oils in 3T3-L1 pre-adipocytes and high fat diet-fed C57BL/6J mice. J. Korean Soc. Food Sci. Nutr. 46(3):279-288 (in Korean).
10.3746/jkfn.2017.46.3.279
7
Kim, Y.R. and S.H. Yoon. 2015. Single cell oil-recent trends in microbial production and utilization. Korean J. Food Sci. Technol. 47(6):687-697 (in Korean).
10.9721/KJFST.2015.47.6.687
8
Kimura, K., M. Yamaoka and Y. Kamisaka. 2004. Rapid estimation of lipid in oleaginous fungi and yeast using Nile red fluorescence. J. Microbiol. Methods 56(3): 331-338.
10.1016/j.mimet.2003.10.01814967224
9
Lee, H. M., H. J. Park, J. N. Kim, W. S. Shin and E. M. Kim. 2017. Monitoring of sulfur dioxide, carotenoid contents and Bacillus cereus contamination of dried sweet potato in circulating dried agricultural products. J. Food Hyg. Saf. 32(6): 477-484 (in Korean).
10.13103/JFHS.2017.32.6.477
10
Lee, J.H. and K.B. Chin. 2012. Evaluation of antioxidant activities of red beet extracts, and physicochemical and microbial changes of ground pork patties containing red beet extracts during refrigerated storage. Korean J. Food Sci. 32(4):497- 503 (in Korean).
10.5851/kosfa.2012.32.4.497
11
Lee, M.S., J.T. Kim, C.J. Kim, Y.J. Cho and Y.H. Kim. 2006. Effects of portulsca oleracea L. extract on lipolysis and hormone sensitive lipase (HSL) gene expression in 3T3-L1 adipocytes. Korean J Nutr. 39(8):742-747 (in Korean).
12
Lim, K.S. 2006. Studies on development of antiobestic beachu Kimchi. Department of Food and Nutrition, MS Thesis, Pusan Univ., Korea (in Korean).
13
Nagao, A. 2009. Absorption and function of dietary carotenoids. Forum Nutr. 61(1): 55-63.
10.1159/00021273819367110
14
Oh, Y.R. 2019. A study on Triglyceride inhibition effect of the red radish (Raphanus sativus L.) sprout extract by using Rhodosporidium toruloides. Department of Oriental Medicine Resources, MS Thesis, Chonbuk National Univ., Korea (in Korean).
15
Park, J.A., K.S. Jin, Y.N. Oh, S.K. Hyun, Y.H. Choi, H.J. Kwon and B.W. Kim. 2013. Antiadipogenic effect of Vitis amurensis root methanol extract and its solvent fractions in 3T3-L1 preadipocytes. Journal of Life Science 23(1):69-78 (in Korean).
10.5352/JLS.2013.23.1.69
16
Sha, Q. 2013. A comparative study on four oleaginous yeasts on their lipid accumulating capacity. The Faculty of Natural Resources and Agricultural Sciences 7:1-29.
17
Sitepu, I.R., L. Ignatia, A.K. Franz, D.M. Wong, S.A. Faulina, M. Tsui, A. Kanti and K. Boundy-Mills. 2012. An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species. J. Microbiol Meth. 91(2):321-328.
10.1016/j.mimet.2012.09.00122985718PMC3478415
18
Sohn, E.H., T.S. Kim, Y.J. Jeong, H.S. Han, Y.S. Lea, Y.M. Cho and S.C. Kang. Triglyceride control effect of agrimonia eupatoria L. in oleic acid induced NAFLD-HepG2 model. 2015. Korean J. Plant Res. 28(5):635-640 (in Korean).
10.7732/kjpr.2015.28.5.635
19
Tiukova, I. A., S. Prigent, M. Sandgren, J. Nielsen and E. J. Kerkhoven. 2019. Genome-scale model of Rhodotorula toruloides metabolism. BioRxiv. 528489.
10.1101/528489
20
Tyrovolas, S. and D.B. Panagiotakos. 2010. The role Mediterranean type of diet on the development of cancer and cardivascular diesease, in the elderly: A systematic review. Maturitas 65(2):122-130.
10.1016/j.maturitas.2009.07.00319656644
21
Wintergerst, E.S., S. Maggini and D.H. Hornig. 2007. Contribution of selected vitamins and trace elements to immune function. Ann. Nutr. Metab. 51(4):301-323.
10.1159/00010767317726308
22
Wolins, N.E., B.K. Quaynor, J.R. Skinner, A. Tzekov, C.W. Park, K.H. Choi and P.E. Bickel. 2006. OP9 mouse stromal cells rapidly differentiate into adipocytes: Characterization of a useful new model of adipogenesis. J. Lipid Res. 47(2): 450-460.
10.1194/jlr.D500037-JLR20016319419
23
Zhang, H.S., E.H. Cao and J.F. Qin. 2003. Intracellularredox status modulates monocyte chemoattractant protein-1 expression stimulated by homocysteine in endothelial cells. J Cardiovasc Pharm. 42(2):258-265.
10.1097/00005344-200308000-0001612883331
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :5
  • Pages :415-422
  • Received Date : 2019-05-15
  • Revised Date : 2019-07-15
  • Accepted Date : 2019-08-20
  • DOI :https://doi.org/10.7732/kjpr.2019.32.5.415
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