Isolation of the Constituent Inhibiting Nitric Oxide formation from Lycopus lucidus in LPS-induced Macrophage Cells

Hee-Juhn Park

doi:10.7732/kjpr.2019.32.4.264

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2019 Vol.32, Issue 4 Preview Page Next Page

Research Article

Isolation of the Constituent Inhibiting Nitric Oxide formation from Lycopus lucidus in LPS-induced Macrophage Cells LPS로 유도한 대식세포에서 Nitric Oxide 생성을 저해하는 쉽싸리 성분의 분리

31 August 2019. pp. 264-269

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Abstract

This research was performed to identify the anti-inflammatory constituent from the herb of Lycorus lucidus (Lamiaceae). The MeOH extract of this plant material and its two fractions, the lipophilic- (CHCl₃ fraction) and the hydrophilic fraction (BuOH fraction), were prepared to test anti-inflammatory activity. For this purpose, the inhibition rate on inducible nitric oxide synthase (iNOS) activity was assessed by determining nitric oxide (NO) formation in lipopolysaccharide (LPS)-induced macrophage 264.7 cells. The CHCl₃ fraction that greatly inhibited nitric NO formation was chromatographed to lead the isolation of ursolic acid. Since ursolic acid inhibited NO formation dose dependently in this study, this compound was considered as one of the active constituent responsible for anti-inflammation. However, the activity of rosmarinic acid isolated from the BuOH fraction was weak.

Keywords

Anti-inflammatory

iNOS

Lamiaceae

Lycorus lucidus

Nitric oxide

Ursolic acid

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Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 32
No :4
Pages :264-269
Received Date : 2019-04-11
Revised Date : 2019-07-01
Accepted Date : 2019-07-10
DOI :https://doi.org/10.7732/kjpr.2019.32.4.264

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

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