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2019 Vol.32, Issue 6 Preview Page

Research Article

The Protective and Inhibitory Effect of Antioxidants Found in Broussonetia kazinoki Siebold against Oxidative DNA Damage
Tae-Won Jang1
,
Ji-Soo Choi2
,
Hoi-Ki Kim3
,
Eun-Ja Lee3
,
Ki-Beom Lee4
,
Tae-Hyung Kwon5
,
Do-Wan Kim6
,
Jeong-Jwa Ahn6
,
Jae-Ho Park7*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Graduate Student, Department of Medicinal Plant Science, Jungwon University, Goesan 28024, Korea
3Researcher, Fanipinkorea Co., Ltd, Cheongju 28162, Korea
4Senior Researcher, Incheon Business Information Technopark. Biotechnology & Business Center, Incheon 21999, Korea
5Senior Researcher, Department of Research and Development, Chuncheon Bioindustry Foundation, Chuncheon 24232, Korea
6Professor, Department of Food Science and Industry, Jungwon University, Goesan 28024, Korea
7Professor, Department of Pharmaceutical Science, Jungwon University, Goesan 28024, Korea
* Corresponding Author
31 December 2019. pp. 714-722
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Abstract

Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.

Keywords
Broussonetia kazinoki Siebold
DNA Damage
p53
γ-H2AX
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :6
  • Pages :714-722
  • Received Date : 2019-10-22
  • Revised Date : 2019-12-03
  • Accepted Date : 2019-12-13
  • DOI :https://doi.org/10.7732/kjpr.2019.32.6.714
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