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2019 Vol.32, Issue 5 Preview Page

Research Article

Single Crossing Condition of Miscanthus sacchariflorus and Miscanthus sinensis to Breed Miscanthus x giganteus Cultivar

이질3배체 억새(Miscanthus x giganteus) 품종육성을 위한 물억새(M. sacchariflorus)와 참억새(M. sinensis) 단교배 조건구명

Youn-Ho Moon1
,
Kwang-Soo Kim1
,
Ji-Eun Lee1
,
Da-Eun Kwon1
,
Yong-Ku Kang2
,
Young-Lok Cha1*
문 윤호1
,
김 광수1
,
이 지은1
,
권 다은1
,
강 용구2
,
차 영록1*
1Researcher, Bioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, Korea
2Senior Researcher, Bioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, Korea
1농촌진흥청 국립식량과학원 바이오에너지작물연구소, 연구사
2농촌진흥청 국립식량과학원 바이오에너지작물연구소, 연구관
* Corresponding Author
31 October 2019. pp. 509-518
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Abstract

This study was conducted to investigate single crossing condition of M. sacchariflrous and M. sinensis for breeding of M. x giganteus cultivar. Compared with natural day length condition, cultivation in short day length condition shorten days to heading to 18~27 days in both species. Pollen germination ratio of were 75.8% at 6 o’clock in M. sacchariflorus and 51.9% at 7 o’clock in M. sinensis but decreased to below 10% at 8 o’clock in both species. When cut ears immerged in 150 mL of cut-flowers conservation solution and isolated with covering of white non-woven fabric, flowering and pollen dispersal were persisted for 7 days, and the ratio of pollen germination were above 40% for 4 days. The ratio of self-fertilization of both species were below 2.5%, but open pollenation ratio were above 50%. We obtained 437 seeds with experimental single cross of 14 combinations between tetraploid M. sacchariflorus and diploid M. siensis by application of developed single crossing methods. In the single cross, numbers of seed set were different by mother plants. Thus, the newly investigated single crossing condition will be used to breed M. x giganteous cultivar which is sterile and has superior characteristics of biomass yield.

Keywords
Bioenergy
Breeding
Miscanthus
Single crossing
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :5
  • Pages :509-518
  • Received Date : 2019-08-08
  • Revised Date : 2019-09-30
  • Accepted Date : 2019-10-07
  • DOI :https://doi.org/10.7732/kjpr.2019.32.5.509
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