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2019 Vol.32, Issue 3

Research Article

Inhibitory Effects of a Combination of Grapefruit and Rosemary Extracts for Alleviating UV-Induced Skin Ageing
Hee-Jeong Choi1
,
Alam Md Badrul2
,
Zhao Peijun1
,
Yeong-Ho Cha3
,
Tae-Ho Kim4
,
Sang-Han Lee5*
1Ph.D. Graduate Student, School of Food Science & Biotechnology, Kyungpook National University
2Post-doc, School of Food Science & Biotechnology, Kyungpook National University
3Master’s Degree Researcher, School of Food Science & Biotechnology, Kyungpook National University
4Research Professor, Biomedical Research Institute, Kyungpook National University Hospital
5Professor, School of Food Science & Biotechnology, Kyungpook National University
* Corresponding Author
30 June 2019. pp. 207-219
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Abstract

Ultraviolet (UV) radiation is associated with the development of extrinsic skin aging. We performed in vivo assays in order to investigate the protective effect of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-induced skin aging. The results indicated that cG&Re displayed elastase inhibitory activity in a dose-dependent manner. Topical application of cG&Re mitigated photo-aging related lesions such as skin erythema and thickening in photo-aged BALB/c mice dorsal skin, by preventing UVB-induced collagen degradation. Immunohistochemical analyses revealed that cG&Re stimulated SIRT-1 expression, and suppressed MMP-1 and IL-1β expression. It was observed that expression of MMP-1 and -13 mRNA was downregulated in the cG&Re-treated group. Furthermore, cG&Re treatment drastically suppressed protein expression of MMP-1 and regulated the phosphorylation of p-38 kinase. As expected, oral administration of cG&Re resulted in the same SIRT-1, MMP-1, and IL-1β expression patterns observed upon topical application of cG&Re in the UV-induced mice model. Overall, the current results demonstrated that cG&Re attenuated both the downregulation of MMP-1 expression and up-regulation of SIRT-1 expression, as well as decreased phosphorylation of MAPK in UVB-induced skin ageing mice model, suggesting that cG&Re might be used as an internal food ingredient for beauty-purposes as well as a functional food material.

Keywords
Combination of grapefruit and rosemary (cG&Re)
MAPKs
MMP-1
Photo-ageing
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :3
  • Pages :207-219
  • Received Date : 2019-02-28
  • Revised Date : 2019-03-20
  • Accepted Date : 2019-04-05
  • DOI :https://doi.org/10.7732/kjpr.2019.32.3.207
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