All Issue

2019 Vol.32, Issue 6 Preview Page

Research Article

Screening of Sclerotinia Rot Resistant Korean Origin Perilla (Perilla frutescens) Germplasm Using a Detached Leaf Method
Ho-Sun Lee1†
,
Afroz Tania2†
,
Young-Ah Jeon3
,
Jung-Sook Sung4
,
Ju-Hee Rhee1
,
Derbie Aseefa Awraris2
,
Jaejong Noh1
,
Aejin Hwang5
,
On-Sook Hur5
,
Na-Young Ro5
,
Jae-Eun Lee5
1Senior Researcher, National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju 54874, Korea
2Post-doc, National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju 54874, Korea
3Researcher, International Technology Cooperation Center, Rural Development Administration, Jeonju 54875, Korea
4Senior Researcher, Upland Crop Breeding, Researcher Division Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
5Researcher, National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju 54874, Korea
* Corresponding Author
† These authors contributed equally to this work.
31 December 2019. pp. 743-751
PDF XML Citation
Abstract

Sclerotinia rot, caused by Sclerotinia sclerotiorum, is a devastating disease that poses a serious threat to perilla production in Korea. Identifying effective sources of resistance offers long term prospects for improving management of this disease. Screening disease resistant genetic resources is important for development of disease-resistant, new cultivars and conduct related research. In the present study, perilla germplasm were screened in vitro against S. sclerotiorum using detached leaf method. Among 544 perilla accessions, two were highly resistant (IT226504, IT226533), five were resistant (IT226561, IT226532, IT226526, IT226441, and IT226589), five were moderately resistant (IT226525, IT226640, IT226568, IT220624, and IT178655), 16 were moderately susceptible, 31 were susceptible, and 485 were highly susceptible. The resistant accessions in this study could serve as resistance donor in the breeding of Sclerotinia rot resistance or subjected to selection procedure of varietal development for direct use by breeders, farmers, researchers, and end consumers.

Keywords
Detached leaf
Perilla frutescens
Resistance
Sclerotinia rot
References
1
Afroz, T., H.S. Lee, Y.A. Jeon, J.S. Sung, J.H. Rhee, D.A. Awraris, J. Noh, A. Hwang, O.S. Hur, N.Y. Ro, J.E. Lee and M.C. Lee. 2019. Evaluation of different inoculation methods for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. J. Crop Sci. Biol. 22:177-183.
10.1007/s12892-019-0115-0
2
Asif, M. 2011. Health effects of omega-3, 6, 9 fatty acids: Perilla frutescens is a good example of plant oils. Orien. Pharm. Exp. Medi. 11:51-59.
10.1007/s13596-011-0002-x21909287PMC3167467
3
Assefa, A.D., Y.J. Jeong, D.J. Kim, Y.A. Jeon, H.C. OK, H.J. Beak and J.S. Sung. 2018. Characterization, identification, and quantification of phenolic compounds using UPLC-Q-TOF-MS and evaluation of antioxidant activity of 73 Perilla frutescens accessions. Food Res. Int. 11:153-167.
10.1016/j.foodres.2018.05.01730007672
4
Bolton, M.D., B.P. Thomma and B.D. Nelson. 2006. Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Mol. Plant Pathol. 7:1-16.
10.1111/j.1364-3703.2005.00316.x20507424
5
Banno, N., T. Akihisa, H. Tokuda, K. Yasukawa, H. Higashihara, M. Ukiya and H. Nishino. 2014. Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects triterpene acids from the leaves of Perilla frutescens. Biosci. Biol. Biochem. 8451(1):85-90.
10.1271/bbb.68.8514745168
6
Boland, G.J. and R. Hall. 1994. Index of plant hosts for Sclerotinia sclerotiorum. Can. J. Bot. 16:93-108.
10.1080/07060669409500766
7
Chen, W. 2007. Detection of QTLs for six yield related traits in oilseed rape (Brassica napus) using DH and immortalized F2 populations. Theor. Appl. Genet. 115:849-858.
10.1007/s00122-007-0613-217665168
8
Chen Y. and D. Wang 2005. Two convenient methods to evaluate soybean for resistance to Sclerotinia sclerotiorum. Plant Dis. 89:1268-1272.
10.1094/PD-89-126830791303
9
Cho, C.T. and B.J. Moon. 1994. Sclerotinia rot of perilla caused by Sclerotinia sclerotiorum (Lib.) de Bary and its new host. Res. Bull. Ins. Agric. Resour. Dong. A Uni. 3:11-24.
10
Choi, Y.J., H.D. Shin and M. Thines. 2009. Two novel Peronospora species are associated with recent reports of downy mildew on sages. Mycol. Res. 113:1340-1350.
10.1016/j.mycres.2009.08.01019699301
11
Del Rio, L.E., N.C. Kurtzweil and C.R. Grau. 2001. Petiole inoculation as a tool to screen soybean germ plasm for resistance to Sclerotinia sclerotiorum. Phytopathol. 91:176.
12
Eckert, G.P., C. Franke, M. Nöldner, O. Rau, M. Wurglics, M. Schubert-Zsilavecz and W.E. Müller. 2010. Plant derived omega-3-fatty acids protect mitochondrial function in the brain. Pharmacol. Res. 61:234-241.
10.1016/j.phrs.2010.01.00520079842
13
Frankel, O.H. 1977. Natural variation and its conservation: In Muhammed, A. and R.C. von Botstel, (eds.), Genet. Divers. Plants. Plenum Press, New York, USA. pp. 21-24.
10.1007/978-1-4684-2886-5_4801593
14
Grau, C.R. and H.L. Bissonette. 1974. Whetzelinia stem rot of soybean in Minnesota. Plant Dis. Rept. 58:693-695.
15
Godoy, M., F. Castano, J. Re and R. Rodriguez. 2005. Sclerotinia resistance in sunflower genotypic variations of hybrids in three environment of Argentina. Euphytica 145:147-154.
10.1007/s10681-005-0627-2
16
Guixiang, Z., T.D. Etherton, K.R. Martin, S.G. West, P.J. Gillies and P.M. Kris-Etherton. 2004. Human nutrition and metabolism dietary α-linolenic acid reduces inflammatory and lipid cardiovascular risk factors in hypercholesterolemic men and women. The J. Nutrition 134(11):2991-2997.
10.1093/jn/134.11.299115514264
17
Ha, T.J., J.H. Lee, M.H. Lee, B.W. Lee, H.S. Kwon, C.H. Park, K. Shim, H. Kim, I. Baek and Jang D.S. 2012. Isolation and identification of phenolic compounds from the seeds of Perilla frutescens (L.) and their inhibitory activities against α-glucosidase and aldose reductase. Food Chem. 135(3): 1397-1403.
10.1016/j.foodchem.2012.05.10422953872
18
Hartman, G.L., M.E. Gardner, T. Hymowitz and G.C. Naidoo. 2000. Evaluation of perennial Glycine species for resistance to soybean fungal pathogens that cause Sclerotinia stem rot and sudden death syndrome. Crop Sci. 40:545-549.
10.2135/cropsci2000.402545x
19
James, M.J., R.A. Gibson and L.G. Cleland. 2000. Dietary polyunsaturated fatty acids and inflammatory mediator production. The American J. Clin. Nutrition 71(S):343-348.
10.1093/ajcn/71.1.343s10617994
20
Kim, E.J., K.J. Sa and J.K. Lee. 2011. Morphological characteristics between the two cultivated types of perilla crop and their weedy types. The Korean Society of Breed. Sci. 4(1):56.
21
Kim, H.S. and B.W. Diers. 2000. Inheritance of partial resistance to Sclerotinia stem rot in soybean. Crop. Sci. 40:55-61.
10.2135/cropsci2000.40155x
22
Kim, M., J.H. Nam, D.H. Oh and Y. Park. 2010. Erythrocyte α -linolenic acid is associated with the risk for mild dementia in Korean elderly. Nutrition Res. 30(11):756-761.
10.1016/j.nutres.2010.10.00521130294
23
Kim, W.G., B.D. Lee, W.D. Cho and D.B. Shin. 2001. Anthracnose of perilla caused by Colletotrichum spp. and Glomerella cingulata. Plant Pathol. J. 17:236-241.
24
Korean Statistical Information Service (KOSIS), Crop Production Survey, 2018. http://kosis.kr/eng/statisticsList/statisticsListIndex.do?menuId=M_01_01&vwcd=MT_ETITLE& parmTabId=M_ 01_01&statId=1962008&themaId=#101_F1H.3 [Retrieved July 20, 2019].
25
Kull, L.S. 2003. Evaluation of Resistance Screening Methods for Sclerotinia stem rot of soybean and dry bean. Plant Dis. 87:1471-1476.
10.1094/PDIS.2003.87.12.147130812389
26
Lee, A.Y., M.H. Lee, S. Lee and E.J. Cho. 2017. Alpha- linolenic acid from perilla frutescens var. japonica oil protects Aβ-induced cognitive impairment through regulation of APP Processing and Aβ degradation. J. Agric. Food Chem. 65(49):10719-10729.
10.1021/acs.jafc.7b0394129092397
27
Lee, H.B., C.J. Kim and H.Y. Mun. 2009. First report of stem blight on perilla (Perilla frutescens) caused by Corynespora cassiicola in Korea. Plant Dis. 93:550.
10.1094/PDIS-93-5-0550A30764152
28
Lee, J.K. and O. Ohnishi. 2003. Genetic relationships among cultivated types of Perilla frutescens and their weedy types in East Asia revealed by AFLP markers. Gen. Res. Crop Evol. 50:65-74.
10.1023/A:1022951002271
29
Liu, S., H. Wang, J. Zhang, B.D.L. Fitt, Z. Xu, N. Evans, Y. Liu, W. Yang and X. Guo. 2005. In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorum. Plant Cell Rep. 24:133-144.
10.1007/s00299-005-0925-015759119
30
Maeng, J.H., Y.R. Yeong, K.S. Kim and S.Y. Ahn. 2009. Comparison of growth and yield of perilla by open field and rain shelter cultivation in east area of Gangwon. Kor. J. Hort. Sci. Techno. 27(Suppl. I):54.
31
Makino, H., Y. Furata, H. Wakushima, H. Fujii, K. Saito and Y. Kano. 2003. Anti-allergic effect of Perilla frutescens and its active constituents. Phytother. Res. 17:240-243.
10.1002/ptr.111512672153
32
Ma, S.J. and J.K. Lee. 2017. Morphological variation of perilla crop and their weedy types from northern and southern areas of China. Genet. Mol. Res. 16(4):gmr16039853.
10.4238/gmr16039853
33
Moon, B.J., S.H. Roh, Y.J. Son, H.S. Kang, J.P. Lee, B.S. Kim and D.S. Chung. 1998. Occurrence of gray mold rot of perilla caused by Botrytis cinerea. Korean J. Plant Pathol.14: 467-472.
34
Miorini, T.J.J., Z.N. Kamvar, R. Higgins, C.G. Raetano, J.R. Steadman and S.E. Everhart. 2018. Variation in pathogen aggression and cultivar performance against Sclerotinia sclerotiorum in soybean and dry bean from Brazil and the U.S. Peer J. Preprints 6: e26622v1.
10.7287/peerj.preprints.26622
35
Mwangi, E.W., S. Marzougui, J.S. Sung, E.C. Bwalya, Y.M. Choi and M.C. Lee. 2019. Assessment of genetic diversity and population structure on Kenyan sunflower (Helianthus annus L.) breeding lines by SSR markers. Korean J. Plant Res. 32(3):244-253.
36
Naher, N., S. Shamsi, M.R. Ali and A.M. Bashar. 2018. Screening of Sclerotinia stem rot resistance in Bangladesh mustard germplasm using cotyledon assay method. Dhaka Univ. J. Biol. Sci. 27:85-92.
37
The Korean Society of Plant Pathology. 2009. List of plant diseases in Korea, fifth edition Seoul. The Korean Society of Plant Pathol. p. 89.
38
Vuong, T.D., D.D. Hoffman, B.W. Diers, J.K. Miller, J.R. Steadman and G.L. Hartman. 2004. Utilization of the cut stem inoculation method to evaluate soybean, dry bean, and sunflower for resistance to Sclerotinia sclerotiorum. Crop Sci. 44:777-783.
10.2135/cropsci2004.7770
39
Woo, S.Y., K.J. Sa and J.K. Lee. 2016. Collection and evaluation of genetic variation of perilla accessions in the Jeju Island. Plant Breed. Biotech. 4(1):87-98.
10.9787/PBB.2016.4.1.87
40
Wu, J., Q. Zhao, Q. Yang, H. Liu, Q. Li, X. Yi, Y. Cheng, L. Guo, C. Fan and Y. Zhou. 2016. Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus. Sci. Rep. 6:19007.
10.1038/srep1900726743436PMC4705546
41
Yang, S.Y., C.O. Hong, H. Lee, S.Y. Park, B.G. Park and K.W. Lee. 2012. Protective effect of extracts of Perilla frutescens treated with sucrose on tert-butyl hydroperoxide-induced oxidative hepatotoxicity in vitro and in vivo. Food Chem. 133:337-343.
10.1016/j.foodchem.2012.01.03725683404
42
Yin, X., B. Yi, W. Chen, W. Zhang, J. Tu, W.G.D. Fernando and T. Fu. 2010. Mapping of QTLs detected in a Brassica napus DH population for resistance to Sclerotinia sclerotiorum in multiple environments. Euphytica 173:25-35.
10.1007/s10681-009-0095-1
43
Yun, H.Y., Y.H. Kim, S.G. Hong and K.J. Lee. 2007. First Description of Coleosporium plectranthi causing perilla rust in Korea. Plant Pathol. J. 23:7-12.
10.5423/PPJ.2007.23.1.007
44
Zhang, T., S. Zhao, W. Li, L. Ma, M. Ding, R. Li and Y. Liu. 2014. High-fat diet from perilla oil induces insulin resistance despite lower serum lipids and increases hepatic fatty acid oxidation in rats. Lip. Health Dis. 13(15):1-9.
10.1186/1476-511X-13-1524422660PMC3904189
45
Zhao, J. and J. Meng. 2003. Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). Theor. Appl. Genet. 106:759-764.
10.1007/s00122-002-1171-212596007
46
Zhao, J., J.A. Udall, P.A. Quijada, C.R. Grau, J. Meng and T.C. Osborn. 2006. Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. Theor. Appl. Genet. 112:509-516.
10.1007/s00122-005-0154-516333614
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :6
  • Pages :743-751
  • Received Date : 2019-08-14
  • Revised Date : 2019-11-29
  • Accepted Date : 2019-12-06
  • DOI :https://doi.org/10.7732/kjpr.2019.32.6.743
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close