All Issue

2019 Vol.32, Issue 2 Preview Page

Research Article

The Disease Severity and Related Pathogens Caused by Root Rot on 6 Years Old Ginseng Cultivation Fields

인삼 6 년근 수확지의 뿌리썩음병 발생현황 및 관련 병원균 동정

Mun Won Seo1
,
You Kyoung Han2
,
Yeoung Seuk Bae3
,
Seung Ho Lee2*
서 문원1
,
한 유경2
,
배 영석3
,
이 승호2*
1Post-doc, Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
2Researcher, Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
3Senior Researcher, Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea
1농촌진흥청 국립원예특작과학원 인삼특작부 인삼과, 연구원
2농촌진흥청 국립원예특작과학원 인삼특작부 인삼과, 연구사
3농촌진흥청 국립원예특작과학원 인삼특작부 인삼과, 연구관
* Corresponding Author
30 April 2019. pp. 144-152
PDF XML Citation
Abstract

Ginseng (Pnanx ginseng C. A. Meyer) is famous worldwide, and is very important cash crop and medicinal herb in Korea. It takes four to five years to produce harvestable ginseng roots, and ginseng is attacked by several pathogens during cultivation. We investigated the disease rate caused by ginseng root rot from 6 years old ginseng cultivation fields (Chungnam; 9 fields, Chungbuk; 11 fields, Gangwon 5 fields). The highest disease severity was Dangjin D (2.9) and the lowest one was Gaesan C (0.6). Of the 625 isolations, 340 isolations were classified as Ilyonectria radicicola and Fusarium solani. Finally, genetic diversity of I. radicicola and F. solani was confirmed by sequence analysis. Among the I. radicicola group, I. mors-panacis, which is known as highly virulent pathogen, and I. liriodendri, I. robusta and I. cyclamicicola, which are weakly virulent pathogens, were identified. In the case of F. solani, it is divided into two groups, but it is necessary to conduct diversity research through genetic analysis and pathogenetic studies using various markers. Based on these results, it could be used as a basic data for control of ginseng root rot pathogens.

Keywords
Disease severity
Fusarium solani
Ginseng
Ilyonectria radicicola
Root rot
References
1
Ahn, C.H. 2017. Effect of fine bubble treatment on the growth of two-year-old ginseng. Korean J. Plant Res. 30(5):549-559.
2
Cabral, A., J.Z. Groenewald, C. Rego, H. Oliveira and P.W. Crous. 2012. Cylindrocarpon root rot: Multi-gene analysis reveals novel species within the Ilyonectria radicicola species complex. Mycol. Progress. 11(3):655-688.
10.1007/s11557-011-0777-7
3
Cho, D.H., K.J. Park, Y.H. Yu, S.H. Ohh and H.S. Lee. 1995. Root-rot development of 2-year old ginseng (Panax ginseng C. A. Meyer) caused by Cylindrocarpon destructans (Zinssm) Scholten in the continuous cultivation field. Korean Journal of Ginseng Science 19:175-180.
4
Chung, H.S. 1972. Ginseng disease in Korea. Korea Pharmacognosy 2:73-79.
5
Doyle, J.J. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15.
6
Kang, S.W., B.Y. Yeon, G.S. Hyeon, Y.S. Bae, S.W. Lee and N.S. Seong. 2007. Changes of soil chemical properties and root injury ratio by progress years of post-harvest in continuous cropping soils of ginseng. Korean J. Medicinal Crop Sci. 15(3):157-161.
7
Kim, J.H. and M.W. Lee. 1974. On the root rot of ginseng (I) - Isolation and identification of Fusarium spp. Korean Journal of Microbiology 12:94-98.
8
Kim, Y.H., J.H. Lee, S.H. Ohh, Y.H. Yu and I.H. Lee. 1993. Ginseng growths in abolished ginseng fields and factors affecting the ginseng growth. Korean J. Ginseng Sci. 17(1):45-51.
9
Lee, S.G. 2004. Fusarium species associated with ginseng (Panax ginseng) and their role in the root-rot of ginseng plant. Res. Plant Dis. 10(4):248-259.
10.5423/RPD.2004.10.4.248
10
Lee, S.W., S.H. Lee, M.W. Seo, I.B. Jang, I.B. Jang, J. You, J.W. Moon and S.J. Seo. 2018. Effect of soil fumigation and maize cultivation on reduction of replant failure in ginseng. Korean J. Medicinal Crop Sci. 26(3):248-253.
11
Matuo, T. and W.C. Snyder. 1972. Host virulence and the hypomyces stage of Fusarium solani f. sp. pisi. Phytopathology 62:731-735.
10.1094/Phyto-62-731
12
Matuo, T. and W.C. Snyder. 1973. Use of morphology and mating populations in the identification of formae speciales in Fusarium solani. Phytopathology 63:562-565.
10.1094/Phyto-63-562
13
Park, G.J. and H.S. Chung. 1997. Characteristics of soil groups based on the development of root rot of ginseng seedlings. Korean J. Plant Pathol. 13:46-56.
14
Park, H.W., T.G. Lim, C.H. Choi and J.E. Choi. 2006. Factors and cause of rusty-ginseng occurrence. Korean J. Crop Sci. 51:396-400.
15
Punja, J.K. 1997. Fungal pathogens of American ginseng (Panax quinquefolius) in British Columbia. Canadian Journal of Plant Pathology 19(3):301-306.
10.1080/07060669709500528
16
Reeleder, R.D. and R.A. Brammall. 1994. Pathogenicity of Pythium species, Cylindrocarpon destructans, and Rhizoctonia solani to ginseng seedlings in Ontario. Canadian Journal of Plant Pathology 16(4):311-316.
10.1080/07060669409500736
17
Reeleder, R.D., R. Roy and B. Capell. 2002. Seed and root rots of ginseng (Panax quinquefolius L) caused Cylindrocarpon destructans and Fusarium spp. Journal of Ginseng Research 26(3):151-158.
10.5142/JGR.2002.26.3.151
18
Seo, M.W. 2009. Analysis of characteristics for Korean ginseng root rot pathogen (Cylindrocarpon destructans) and development of new diagnostic molecular marker. Department of Agricultural Biology, Master Thesis, Chungnam National Univ., Korea. pp. 28-35.
19
Seo, M.W. 2017a. Reclassification and disease occurrence factor in first plantd field of Korean ginseng root rot pathogen population (Cylindrocarpon destructans) and practical disease diagnosis. Department of Agricultural Biology, Ph.D. Thesis, Chungnam National Univ., Korea. pp. 28-34.
20
Seo, M.W., J.Y. Song, K.H. Kang, S.Y. Park, S.I. Kim and H.G. Kim. 2017b. Virulence assays and genetic reclassification to assess the pathogenicity of Cylindrocarpon destructans isolated from peony in ginseng. The Korean Journal of Mycology 45(2):132-138.
10.4489/KJM.20170016
21
Seo, M.W., J.Y. Song, S.I. Kim, S.K. Oh and H.G. Kim. 2018. Pathogenicity on ginseng and sequence assys of Ilyonectria radicicola isolated from chestnut rhizosphere soils. Korean J. Medicinal Crop Sci. 26(4):302-307.
22
Shin, J.H., B.D. Yon, H.J. Kim, S.J. Kim and D.Y. Chung. 2012. Soil environment and soil-borne plant pathogen causing root rot disease of ginseng. Korean J. Soil Sci. Fert. 45(3):370-376.
10.7745/KJSSF.2012.45.3.370
23
Singleton, L.L., J.D. Mihail and C.M. Rush. 1992. Methods for research on soilborne phytopathogenic fungi: In Jeanne, M. et al., ASP Press. St. Paul. MN (USA). pp. 140-141.
10.2307/3760494
24
Suga, H., T. Hasegawa, H. Mitsui, K. Kageyama and M. Hyakumachi. 2000. Phylogenetic analysis of the phytopathogenic fungus Fusarium solani based on the rDNA-ITS region. Mycol. Res. 104(10):1175-1183.
10.1017/S0953756200002719
25
Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30(12):2725-2729.
10.1093/molbev/mst19724132122PMC3840312
26
The Korean Society of Plant Pathology (KSPP). 2009. List of plant disease in Korea. The Korean Society of Plant Pathology. Seoul, Korea. pp. 91, 284, 288.
27
Yu, Y.H., D.H. Cho, I.H. Lee and S.H. Ohh. 1990. Effect of seedling depth on severity of damping-off ginseng sedlings caused by Rhizoctonia solani. Korean J. Ginseng Sci. 14(3):432-436.
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :2
  • Pages :144-152
  • Received Date : 2018-11-21
  • Revised Date : 2019-02-19
  • Accepted Date : 2019-03-27
  • DOI :https://doi.org/10.7732/kjpr.2019.32.2.144
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close