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2021 Vol.34, Issue 5 Preview Page

Research Article

Immuno-enhancing and Anti-obesity Effect of Abelmoschus manihot Root Extracts

금화규(Abelmoschus manihot) 뿌리 추출물의 면역증진 및 항비만효과

Ju Hyeong Yu1
,
Na Gyeong Geum1
,
Joo Ho Ye1
,
Jin Boo Jeong2*
유 주형1
,
금 나경1
,
여 주호1
,
정 진부2*
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Professor, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
1국립안동대학교 생약자원학과, 대학원생
2국립안동대학교 생약자원학과, 교수
*Corresponding Author
1 October 2021. pp. 411-419
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Abstract

In this study, we investigated in vitro immune-enhancing and anti-obesity activity of Abelmoschus manihot roots (AMR) in mouse macrophage RAW264.7 cells and mouse adipocytes 3T3-L1 cells. AMR increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in RAW264.7 cells. The inhibition of toll like receptor (TLR) 2 and 4 blocked AMR-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of mitogen-activated protein kinases (MAPKs) signaling pathway reduced AMR-mediated production of immunostimulatory factors. From these results, AMR is considered to have immune-enhancing activity through TLR2/4-mediated activation of MAPKs signaling pathway. In addition, AMR inhibited lipid accumulation and reduced the protein level such as CCAAT enhancer-binding protein alpha (CEBPα), peroxisome proliferator-activated receptor gamma (PPARγ), perilipin-1, adiponectin and fatty acid binding protein 4 (FABP4) associated with lipid accumulation in 3T3-L1 cells, indicating that AMR may have anti-obesity activity. Based on these results, AMR is expected to be used as a potential functional agent for immune enhancement and anti-obesity.

Keywords
Adipocytes
Abelmoschus manihot
Anti-obesity activity
Immune-enhancement
Macrophage
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :5
  • Pages :411-419
  • Received Date : 2021-07-09
  • Revised Date : 2021-07-22
  • Accepted Date : 2021-05-08
  • DOI :https://doi.org/10.7732/kjpr.2021.34.5.411
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