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2019 Vol.32, Issue 5 Preview Page

Research Article

Genetic Identification and Phylogenic Analysis of New Varieties and 149 Korean Cultivars using 27 InDel Markers Selected from Dense Variation Blocks in Soybean (Glycine max (L.) Merrill)

변이밀집영역 유래 27개 InDel 마커를 이용한 콩(Glycine max (L.) Merrill) 신품종 판별 및 국내 149 품종과 유연관계 분석

JaeBuhm Chun1
,
Mina Jin2*
,
Namhee Jeong3
,
Chuloh Cho3
,
Mi-Suk Seo1
,
Man-Soo Choi1
,
Dool-Yi Kim1
,
Hwang-Bae Sohn4
,
Yul-Ho Kim5
전 재범1
,
진 민아2*
,
정 남희3
,
조 철오3
,
서 미숙1
,
최 만수1
,
김 둘이1
,
손 황배4
,
김 율호5
1Resercher, Crop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea
2Senior Researcher, Crop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea
3Post-doc, Crop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea
4Resercher, Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
5Senior Researcher, Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
1농촌진흥청 국립식량과학원 작물기초기반과, 농업연구사
2농촌진흥청 국립식량과학원 작물기초기반과, 박사후연구원
3농촌진흥청 국립식량과학원 작물기초기반과, 박사후연구원
4국립식량과학원 고령지농업연구소, 농업연구사
5국립식량과학원 고령지농업연구소, 농업연구관
* Corresponding Author
31 October 2019. pp. 519-542
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Abstract

Twenty soybean cultivars developed recently were assessed using 27 insertion and deletion (InDel) markers derived from dense variation blocks (dVBs) of soybean genome. The objective of this study is to identify the distinctness and genetic relationships among a total of 169 soybean accessions including new cultivars. The genetic homology between 149 accessions in the soybean barcode system and 20 new cultivars was 61.3% on average with the range from 25.9% to 96.3%, demonstrating the versatile application of these markers for cultivars identification. The phylogenic analysis revealed four subgroups related to their usage. The 80% of cultivars for vegetable and early maturity and the 65.9% of cultivars for bean sprouts were clustered in subgroup I-2 and II-2, respectively, indicating of the limited gene pools of their crossing parents in breeding. On the other hands, the cultivars for soy sauce and tofu with considerable gene flow by genome reshuffling were distributed evenly to several subgroups, I-1 (44.4%), I-2 (26.4%) and II-2 (23.6%). We believe that the 27 InDel markers specific to dVBs can be used not only for cultivar identification and genetic diversity, but also in breeding purposes such as introduction of genetic resources and selection of breeding lines with target traits.

Keywords
Barcode system
Genetic identification
InDel marker
Soybean cultivar
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :5
  • Pages :519-542
  • Received Date : 2019-09-21
  • Revised Date : 2019-10-04
  • Accepted Date : 2019-10-10
  • DOI :https://doi.org/10.7732/kjpr.2019.32.5.519
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