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2018 Vol.31, Issue 4 Preview Page

Research Article

Multiple Shoot Induction and Bulb Mass Proliferation System by in Vitro Immature Spathe Culture of Elephant Garlic (Allium ampeloprasum L.)

코끼리마늘(Allium ampeloprasum L.)의 기내 미숙총포 배양을 통한 다신초유도와 종구대량증식 시스템

Young Hee Kwon1*
,
Jae Hyun Jeong1
,
Jae Sun Lee1
,
Jong Ok Jeon1
,
Young Uk Park1
,
Ji Hyun Min1
,
Who Bong Chang1
,
Sang Young Lee1
,
Cheol Ku Youn1
,
Ki Hyun Kim1
권 영희1*
,
정 재현1
,
이 재선1
,
전 종옥1
,
박 영욱1
,
민 지현1
,
장 후봉1
,
이 상영1
,
윤 철구1
,
김 기현1
1Chungcheongbuk-do Agricultural Research & Extension Services
1충청북도농업기술원
* Corresponding Author
31 August 2018. pp. 355-362
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Abstract

This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 ㎝ length was cultured right after harvesting. In MS medium supplemented with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 ㎝, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the addition of 30 ㎎/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.

Keywords
Bulb
Elephant garlic (Allium ampeloprasum L.)
Immature spathe
in vitro
Propagation
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :4
  • Pages :355-362
  • Received Date : 2017-11-09
  • Revised Date : 2018-03-02
  • Accepted Date : 2018-03-13
  • DOI :https://doi.org/10.7732/kjpr.2018.31.4.355
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