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2019 Vol.32, Issue 6 Preview Page

Research Article

Cryopreservation of in vitro Grown Shoot Tips of Strawberry (Fragaria × ananassa Duch.) Genetic Resources by Droplet-vitrification
Young-Yi Lee1*
,
Balaraju Kotnala2
,
Jae-Young Song2
,
Jung-Yoon Yi1
,
Sun-Yi Lee3
,
Jung-Ro Lee1
,
Munsup Yoon4
,
Haeng-Hoon Kim5
1Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
2Post-doc, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
3Researcher, Department of Horticultural Crop Research, NIHHS, RDA, Wanju 55365, Korea
4Senior Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
5Professor, Department of Well-being Resources, Sunchon National University, Sunchon 57922, Korea
* Corresponding Author
31 December 2019. pp. 689-697
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Abstract

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. ‘Wonkyo3114’ and ‘Gurumi40’. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 µL PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.

Keywords
Cryopreservation
Dehydration
Regrowth
Strawberry
Shoot tips
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :6
  • Pages :689-697
  • Received Date : 2019-09-03
  • Revised Date : 2019-12-02
  • Accepted Date : 2019-12-04
  • DOI :https://doi.org/10.7732/kjpr.2019.32.6.689
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