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Research Article

In vitro Multiplication of Hosta Tratt. Species Native to Korea by Shoot-tip Culture

경정배양에 의한 한국 자생 비비추속 식물의 기내증식

Han Choi1*
,
Jong Cheol Yang1*
,
Sun Hee Ryu1
,
Sae Mi Yoon1
,
Sang Yong Kim2
,
Seung Youn Lee1**
최 한1*
,
양 종철1*
,
류 선희1
,
윤 새미1
,
김 상용2
,
이 승연1**
1Researcher, Useful Plant Resources Center, Korea National Arboretum, Yangpyeong 12519, Korea
2Senior Researcher, Useful Plant Resources Center, Korea National Arboretum, Yangpyeong 12519, Korea
1국립수목원 유용식물증식센터, 연구원
2국립수목원 유용식물증식센터, 연구관
* First Author
** Corresponding Author
28 February 2019. pp. 53-62
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Abstract

The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 ㎎/L BA with 0.1 ㎎/L NAA, 0.1, 0.5, 1.0, 2.0 ㎎/L TDZ with 0.1 ㎎/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 ㎎/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 ㎎/L BA in H. minor (11.1), 1.0 ㎎/L BA and 0.1 ㎎/L TDZ in H. venusta (8.1), and 0.5 ㎎/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

Keywords
Crown bud
Cytokinin
Hosta
Shoot tip
Somatic embryo
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :1
  • Pages :53-62
  • Received Date : 2018-09-15
  • Revised Date : 2018-12-18
  • Accepted Date : 2018-12-28
  • DOI :https://doi.org/10.7732/kjpr.2019.32.1.053
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