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2018 Vol.31, Issue 6 Preview Page

Research Article

A Successful Regeneration from Shoot Tips of Chrysanthemum morifolium (Ramat.) following Cryopreservation by Droplet-vitrification
Jung-Yoon Yi1
,
Kotnala Balaraju2
,
Hyung-Jin Baek3
,
Mun-Seop Yoon3
,
Haeng-Hoon Kim4
,
Young-Yi Lee1*
1Researcher, National Agrobiodiversity Center, NAS, RDA
2Post-doc, National Agrobiodiversity Center, NAS, RDA
3Senior Researcher, National Agrobiodiversity Center, NAS, RDA
4Professor, Department of Well-being Resources, Sunchon National University
*Corresponding Author
31 December 2018. pp. 675-683
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Abstract

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of Chrysanthemum morifolium (Ramat.) cvs. ‘Borami’ and ‘Yes morning’. The shoot tips of Chrysanthemum were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7 M). Precultured explants were treated with loading solution (LS, C6) containing glycerol 20% and sucrose 20% for 30 min and exposed to dehydration solution (B5) containing 40% of glycerol and 40% of sucrose for 60 min at 25℃, and then transferred onto droplets containing 2.5 ㎕ PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposing of shoot tips to MS + 0.3M sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at 25℃ in both the cultivars. The viability of cooled samples, followed by culturing on NH4NO3-free MS medium for first 5 days was increased to two-fold (80.7%) regrowth rate over those cultured on normal MS medium or MS medium containing plant growth regulators. This result shows droplet-vitrification would be a promising method for cryobanking chrysanthemum germplasm.

Keywords
Chrysanthemum
Cryopreservation
Liquid nitrogen
Regrowth
Vitrification
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :675-683
  • Received Date : 2018-11-12
  • Revised Date : 2018-12-23
  • Accepted Date : 2018-12-23
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.675
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