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2019 Vol.32, Issue 4 Preview Page

Research Article

Inhibitory Effects of Pine Cone (Pinus densiflora) on Melanogenesis in B16F10 Melanoma Cells
Seung-Hyun Lee1*
,
Tae-Won Jang2*
,
Ji-Soo Choi3
,
Jeong-Yun Mun4
,
Jae-Ho Park5**
1Professor, Industry-Academia Cooperation Foundation, Jungwon University, Goesan 28024, Korea
2Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
3Graduate Student, Department of Medicinal Plant Science, Jungwon University, Goesan 28024, Korea
4Student, Department of Pharmaceutical Science, Jungwon University, Goesan 28024, Korea
5Professor, Department of Pharmaceutical Science, Jungwon University, Goesan 28024, Korea
* First Author
** Corresponding Author
31 August 2019. pp. 275-281
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Abstract

The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than 100 ㎍/mL. PEF inhibited the expression of microphthalmia- associated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl- 1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.

Keywords
B16F10
Melanogenesis
Pine cone
Pinus densiflora
References
1
Ali, A. 2010. Dermatology A Pictorial Review. McGraw-Hill Education. ISBN978-0-07-179323-0.
2
Busca, R. and R. Ballotti. 2000. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 13(2):60-69.
10.1034/j.1600-0749.2000.130203.x10841026
3
Choi, M.Y., E.Y. Choi, E. Lee, T.J. Rhim, B.C. Cha and H.J. Park. 1997. Antimicrobial activities of pine needle (Pinus densiflora Sieb et Zucc) extract. Korean J. Microbiol. Biotechnol. 25(3):293-297.
4
Chung, H.J., G.H. Hwang, M.J. Yoo and S.J. Rhee. 1996. Chemical composition of pine sprouts and pine needles for the production of pine sprout tea. J. Korean Soc. Food Cult. 11(5):635-641.
5
Chung, J.H., V.N. Hanft and S. Kang. 2003. Aging and photoaging. J. Am. Acad. Dermatol. 49(4):690-697.
10.1067/S0190-9622(03)02127-3
6
Eberlein-Konig, B., M. Placzek and B. Przybilla. 1998. Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and d-α-tocopherol (vitamin E). J. Am. Dermatol. 38(1):45-48.
10.1016/S0190-9622(98)70537-7
7
Fisher, G.J., S. Kang, J. Varani, Z. Bata-Csorgo, Y. Wan, S. Datta and J.J. Voorhees. 2002. Mechanisms of photoaging and chronological skin aging. Arch. Dermatol. 138(11): 1462-1470.
10.1001/archderm.138.11.146212437452
8
Garraway, L.A., H.R. Widlund, M.A. Rubin, G. Getz, A.J. Berger, S. Ramaswamy, R. Beroukhim, D.A. Milner, S.R. Granter, J. Du, C. Lee, S.N. Wagner, C. Li, T.R. Golub D.L. Rimm, M.L. Meyerson, D.E. Fisher and W.R. Sellers. 2005. Integrative genomic analyses identify MITF as a lineage survival oncogene amplified in malignant melanoma. Nature 2005:436(7047):117-122.
10.1038/nature0366416001072
9
Hasegawa, K., R. Furuya, H. Mizuno, K. Umishio, M. Suetsugu and K. Sato. 2010. Inhibitory effect of Elephantopus mollis H.B. and K. extract on melanogenesis in B16 murine melanoma cells by downregulating microphthalmia-associated transcription factor expression. Biosci. Biotechnol. Biochem. 74(9):1908-1912.
10.1271/bbb.10031820834163
10
Hearing, V. J. and K. Tsukamoto. 1991. Enzymatic control of pigmentation in mammals. FASEB J. 5(14):2902-2909.
10.1096/fasebj.5.14.17523581752358
11
Im, S., O. Moro, F. Peng, E.F. Medrano, J. Cormelius, G. Babcock, J.J. Nordlund and Z.A. Abdel-Malek. 1998. Activation of the cyclic AMP pathway by alpha-melanotropin mediates the response of human melanocytes to ultraviolet B radiation. Cancer Res. 58(1):47-54.
12
Jang, T.W., S.H. Nam and J.H. Park. 2016. Antioxidant activity and inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from cone of red pine (Pinus densiflora). Korean J. Plant Res. 29(2):163-170.
10.7732/kjpr.2016.29.2.163
13
Jiang, Z., J. Xu, M. Long, Z. Tu, G. Yang and G. He. 2009. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) induces melanogenesis in B16 cells by MAP kinase activation and tyrosinase upregulation. Life Sci. 85(9-10): 345-350.
10.1016/j.lfs.2009.05.02219527735
14
Jimenez-Cervantes, C., F. Solano, T. Kobayashi, K. Urabe, V.J. Hearing, J.A. Lozano and J.C. Garcia-Borron. 1994. A new enzymatic function in the melanogenic pathway. The 5,6- dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase- related protein-1 (TRP-1). J. Biol. Chem. 269(27):7993-18000.
15
Kim, B., Y.E. Choi and H.S. Kim. 2014. Eruca sativa and its flavonoid components, quercetin and isorhamnetin, improve skin barrier function by activation of peroxisome proliferator- activated receptor (PPAR)-α and suppression of inflammatory cytokines. Phytother. Res. 28(9):1359-1366.
10.1002/ptr.513824610745
16
Lee, A.R., S.S. Roh, E.S. Lee and Y.H. Min. 2016. Anti-oxidant and anti-melanogenic activity of the methanol extract of pine cone. Asian J. Beauty Cosmetol. 14(3):301-308.
10.20402/ajbc.2016.0055
17
Lee, C.B. 2003. An Illustrated Guide to Korean Flora. Hyangmunsa. Publishing Co., Seoul, Korea. p. 141 (in Korean).
18
Onkoksoong, T., S. Jeayeng, N. Poungvarin, S. Limsaengurai, O. Thamsermsang, P. Tripatara, P. Akarasereenont and U. Panich. 2018. Thai herbal antipyretic 22 formula (APF22) inhibits UVA-mediated melanogenesis through activation of Nrf2-regulated antioxidant defense. Phytother. Res. 32(8): 1546-1554.
10.1002/ptr.608329672960
19
Regnault, M.R., N. Gadaud, S. Boulinguez, E. Tournier, L. Lamant, L. Gladieff, H. Roche, S. Guenounou, C. Recher and V. Sibaud. 2015. Chemotherapy-related reticulate hyperpigmentation: a case series and review of the literature. Dermatology 231(4):312-318.
10.1159/00043904726422424
20
Sung, K.C. and K.J. Kim. 2005. Tyrosinase activated inhibition effect and analysis of pine-needle extract. J. Korean Appl. Sci. Technol. 22(1):71-76.
21
Widlund, H.R. and D.E. Fisher. 2003. Microphthalamia-associated transcription factor: a critical regulator of pigment cell development and survival. Oncogene 22(20):3035-3041.
10.1038/sj.onc.120644312789278
22
Ye, Y., J.H. Chu, H. Wang, H. Xu, G.X. Chou, A.K. Leung, W.F. Fong and Z.L. Yu. 2010. Involvement of p38 MAPK signaling pathway in the anti-melanogenic effect of San-bai- tang, a Chinese herbal formula, in B16 cells. J Ethnopharmacol. 132(2):533-535.
10.1016/j.jep.2010.09.00720837127
23
Yoo, J.H., J.Y. Cha, Y.K. Jeong, K.T. Chung and Y.S. Cho. 2004. Antioxidative effect of pine (Pinus densiflora) needle extracts. J. Life Sci. 14(5):863-867.
10.5352/JLS.2004.14.5.863
24
Zhou, J., T. Ren, Y. Li, A. Cheng, W. Xie, L. Xu, L. Peng, J. Lin, L. Lian, Y. Diao, X. Jin and L. Yang. 2017. Oleoylethanolamide inhibits α-melanocyte stimulating hormone stimulated melanogenesis via ERK, Akt and CREB signaling pathways in B16 melanoma cells. Oncotarget 8(34):56868- 56879.
10.18632/oncotarget.1809728915638PMC5593609
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :4
  • Pages :275-281
  • Received Date : 2019-06-11
  • Revised Date : 2019-07-19
  • Accepted Date : 2019-07-22
  • DOI :https://doi.org/10.7732/kjpr.2019.32.4.275
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