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2018 Vol.31, Issue 6 Preview Page

Research Article

Anti-Inflammatory Activity of Acacia Honey through Inhibition of NF-κB and MAPK/ATF2 Signaling Pathway in LPS-Stimulated RAW264.7 Cells
Ha Na Kim1*
,
Kun Ho Son2*
,
Hyung Jin Jeong3*
,
Su Bin Park1
,
Jeong Dong Kim4
,
Jin Boo Jeong3**
1Graduate Student, Department of Medicinal Plant Resources, Andong National University
2Professor, Department of Food and Nutrition, Andong National University
3Professor, Department of Medicinal Plant Resources, Andong National University
4Undergraduate Student, Department of Medicinal Plant Resources, Andong National University
*First Author
**Corresponding Author
31 December 2018. pp. 612-621
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Abstract

Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of IL-1β, IL-6 and TNF-α as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated IκB-α degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of NF-κB activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, TNF-α, IL-6, IL-1β and MCP-1 via interruption of the NF-κB and MAPK/ATF2 signaling pathways.

Keywords
Acacia honey
Anti-inflammation
Honey
Pro-inflammatory mediators
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :612-621
  • Received Date : 2018-09-18
  • Revised Date : 2018-11-13
  • Accepted Date : 2018-11-13
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.612
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