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2020 Vol.33, Issue 6 Preview Page

Research Article

Preculture Condition for Stable Recovery of Strawberry (Fragaria × ananassa Duch.) Shoot Tips after Cryopreservation Using Droplet Vitrification
Young-Yi Lee1†
,
Sun-Yi Lee2
,
Jae-Young Song3
,
Munsup Yoon4
,
Jung-Yoon Yi1
,
Jung-Ro Lee1
,
Haeng-Hoon Kim5
1Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
2Researcher, Department of Horticultural Crop Research, NIHHS, RDA, Wanju 55365, Korea
3Post-doc, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
4Senior Researcher, National Agrobiodiversity Center, NAS, RDA, Suwon 16613, Korea
5Professor, Department of Well-being Resources, Sunchon National University, Sunchon 57922, Korea
*youngyi@korea.kr
1 December 2020. pp. 675-681
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Abstract

This study describes an efficient and stable droplet vitrification following cryopreservation of strawberry shoot tip (Fragaria × ananassa Duch.) accessions ‘Massey’ and ‘MDUS3816’. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 17.5% glycerol and 17.5% sucrose for 40 min and exposed to dehydration solution (B1) containing 50% glycerol and 50% sucrose for 40 min at 25oC. Subsequently, the explants were transferred onto droplets containing 2.5 µL PVS3 on sterilized aluminum foils (4 ㎝× 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with 0.3M sucrose for 30 h + 0.5M sucrose for 16 h at 25oC. The cryopreserved shoots tips exhibited 57.8 % recovery rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks. Variation was not observed in both of ploidy analysis and morphological investigation on plantlets of two accessions cryopreserved under variable preculture conditions.

Keywords
Cryopreservation
Preculture
Strawberry
Shoot tips
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :6
  • Pages :675-681
  • Received Date : 2020-11-05
  • Revised Date : 2020-11-23
  • Accepted Date : 2020-11-24
  • DOI :https://doi.org/10.7732/kjpr.2020.33.6.675
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