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2022 Vol.35, Issue 5

Research Article

Anti-inflammatory and Antioxidative Effects of Lotus Root Extract in LPS-PG-Stimulated Human Gingival Fibroblast-1 Cells

치주염 원인균 LPS-PG로 유도된 인체 치은섬유아세포에서 연뿌리 추출물에 대한 항염증 및 항산화 효과

Young-Kyung Lee1†
,
Chul Hwan Kim1†
,
Dae Won Jeong1
,
Ki Won Lee2
,
Young Taek Oh1
,
Jeong Il Kim2
,
Jin-Woo Jeong3*
이 영경1†
,
김 철환1†
,
정 대원1
,
이 기원2
,
오 영택1
,
김 정일2
,
정 진우3*
1Researcher, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea
2Researcher and Professor, Department of Occupational and Environmental Medicine, College of Medicine, Dong-A University, Busan 49201, Korea
3General Manager, Honam National Institute of Biological Resources, Mokpo 587262, Korea
1국립낙동강생물자원관, 연구원
2동아대학교 의과대학 직업환경의학교실, 교수 및 연구원
3국립호남권생물자원관, 책임연구원
*Corresponding Author
†These authors contributed equally to this work.
1 October 2022. pp. 565-573
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Abstract

Gingival inflammation is one of the main causes that can be related to various periodontal diseases. Human gingival fibroblast (HGF) is the major constituent in periodontal connective tissue and secretes various inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), upon lipopolysaccharide stimulation. This study is aimed at investigating the anti-inflammatory and antioxidative activities of Lotus Root extract (LRE) in Porphyromonas gingivalis derived lipopolysaccharide (LPS-PG)-stimulated HGF-1 cells. The concentration of NO and PGE2, as well as their responsible enzymes, inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2), was analyzed by Griess reaction, ELISA, and western blot analysis. LPS-PG sharply elevated the production and protein expression of inflammatory mediators, which were significantly attenuated by LRE treatment in a dose-dependent manner. LRE treatment also suppressed activation of Toll-like receptor 4 (TLR4)/myeloid differentiation primary response gene 88 (MyD88) and nuclear factor-κB (NF-κB) in LPS-PG-stimulated HGF-1 cells. In addition, one of phase II enzyme, NAD(P)H quinone dehydrogenase (NQO)-1, and its transcription factor, Nuclear factor erythroid 2-related factor 2 (Nrf2), were significantly induced by LRE treatment. Consequently, these results suggest that LRE ameliorates LPS-PG-induced inflammatory responses by attenuating TLR4/MyD88-mediated NF-κB, and activating NQO-1/Nrf2 antioxidant response element signaling pathways in HGF-1 cells.

Keywords
Anti-inflammatory and antioxidative effects
HGF-1 cells
Lotus root
LPS-PG
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 35
  • No :5
  • Pages :565-573
  • Received Date : 2022-07-21
  • Revised Date : 2022-08-01
  • Accepted Date : 2022-08-03
  • DOI :https://doi.org/10.7732/kjpr.2022.35.5.565
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