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2022 Vol.35, Issue 4 Preview Page
Plantlet Regeneration and PLBs Propagation of Bulbophyllum auricomum Lindl.
Theingi Aung Win1†
,
Tung Lian Thang2
,
Phyo Aung Zaw3
,
Keuk Soo Bang14†
,
Seung Hwa Baek5*
1Graduate Student, Department of Lifestyle Medicine, Jeonbuk National University, Iksan 54596, Korea
2Professor, Useful Plant Resource Center. Dagon University, Dagon Myothit Township (East), Yangon 11451, Myanmar
3Researcher, Useful Plant Resource Center. Dagon University, Dagon Myothit Township (East), Yangon 11451, Myanmar
4Professor, Department of Oriental Medicine Resources, Jeonbuk National University, Iksan 54596, Korea
5Professor, Department of Biofood Science and Biotechnology / Department of Culinary and Baking, Chungbuk Provincial University, Okcheon 29046, Korea
*Corresponding Author
†These authors contributed equally to this work.
1 August 2022. pp. 508-514
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Abstract

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 ㎎/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 ㎎/L BAP and 1.0 ㎎/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 ㎎/L BAP and 1.0 ㎎/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

Keywords
Bulbophyllum auricomum Lindl
Coconut water
pH
Suspension culture
Temperature
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 35
  • No :4
  • Pages :508-514
  • Received Date : 2022-05-11
  • Revised Date : 2022-05-30
  • Accepted Date : 2022-06-08
  • DOI :https://doi.org/10.7732/kjpr.2022.35.4.508
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