All Issue

2018 Vol.31, Issue 4 Preview Page

Research Article

Antioxidant and Anti-inflammatory Effects of Ficus erecta var. sieboldii Leaf Extract in Murine Macrophage RAW 264.7 Cells

좁은잎천선과나무(Ficus erecta var. sieboldii) 잎 추출물이 대식세포 RAW 264.7 세포에서 미치는 항산화 및 항염증 효과

Yong-Hwan Jung1*
,
Young-Min Ham1
,
Seon-A Yoon1
,
Dae-Ju Oh1
,
Chang-Suk Kim1
,
Weon-Jong Yoon1
정 용환1*
,
함 영민1
,
윤 선아1
,
오 대주1
,
김 창숙1
,
윤 원종1
1Biodiversity Research Institute, Jeju Technopark (JTP)
1(재)제주테크노파크 생물종다양성연구소
* Corresponding Author
31 August 2018. pp. 303-311
PDF XML Citation
Abstract

In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, CH2Cl2, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, PGE2, IL-6, and IL-β) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the CH2Cl2 and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and CH2Cl2 fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and PGE2). The CH2Cl2 fraction also inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.

Keywords
Antioxidant
Anti-inflammatory
Ficus erecta var. sieboldii
Oxidant stresses
Pro-inflammatory factors
References
1
Ahn, D.K. 1998. Illustrated Book of Korean Medicinal Herbs. Kyohaksa Publishing Co., Seoul, Korea. p. 855 (in Korean).
2
Bae, S.J. 2002. The effects of anticarcinogenic activity of Solanum thberosum peel fractions. J. Korean Soc. Food Sci. Nutr. 31:905-909.
10.3746/jkfn.2002.31.5.905
3
Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1198-1200.
10.1038/1811199a0
4
Branen, A.L. 1975. Toxicological and biochemistry by the use of a stable free radical. Nature 181:1199-1202.
5
Carocho, M. and I.C. Ferreira. 2013. A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methologies and future perspectives. Food Chem. Toxicol. 51:15-25.
10.1016/j.fct.2012.09.02123017782
6
Chang, Z.J., S.C. Kuo, S.C. Chan, F.N. KO and C.M. Teng. 1998. Antioxidant properties of butein isolated from Dalbergia odorifera. Biochem. Biophys. Acta1392:291-299.
10.1016/S0005-2760(98)00043-5
7
Coleman, M.D., S. Fernandes and L.A. Khanderia. 2003. A preliminary evaluation of a novel method to monitor a triple antioxidant combination (vitamin E, C and α-lipoic acid) in diabetic volunteers using in vitro methaemoglobin formation. Environ. Toxicol. Pharmacol. 14:69-75.
10.1016/S1382-6689(03)00027-9
8
Corl, M.M. 1974. Antioxidant activity of tocopherol and ascorbylpalmitate and their mode of action. J. Am. Oil Chem. Soc. 51:321-325.
10.1007/BF02633006
9
Fridovich, I. 1970. Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase. J. Biol. Chem. 245:4053-4057.
5496991
10
Halliwell, B. and J.M.C. Gutteridge. 1993. Free Radicals in Biology and Medicine, 2nd ed. Glarendon Press, Oxford, UK.
11
Heo, S.J., W.J. Yoon, K.N. Kim, G.N. Ahn, S.M. Song, D.H. Kang, A. Affan, C. Oh, W.K. Jung and Y.J. Jeon. 2010. Evaluation of anti-inflammatory effect of fucoxanthin isolation from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages. Food Chem. Toxicol. 48:2045-2051.
10.1016/j.fct.2010.05.00320457205
12
Hyun, E.A., H.J. Lee, W.J. Yoon, S.Y. Park, H.K. Kang, S.J. Kim and E.S. Yoo. 2004. Inhibitory Effect of Salcia officinalis on the inflammatory cytokines and inducible nitric oxide synthesis in murine macrophage RAW 264.7. YAKHAK HOEJI 48:159-164.
13
Ismaki, P. and J. Punnonen. 1997. Pro-and anti-inflammatory cytokine in rheumatoid arthritis. Ann. Med. 29:449-507.
10.3109/07853899709007474
14
Jang, T.W., S.H. Nam and J.H. Park. 2016. Antioxidant activity and inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from cone of red pine (Pinus densiflora). Korean J. Plant Res. 29:163-170 (in Korean).
10.7732/kjpr.2016.29.2.163
15
Kim, E.Y. and K.D. Moudgil. 2008. Regulation of autoimmune inflammation by pro-inflammatory cytokines. Immunol. Left. 120:1-5.
10.1016/j.imlet.2008.07.00818694783PMC2577081
16
Kim, J.H., R.A. Bachmann and J. Chen. 2009. Interleukin-6 and insulin resistance. Vitam. Horm. 80:613-633.
10.1016/S0083-6729(08)00621-3
17
Korycka-Dahl, M., T. Richardson and C. Hicks. 1979. Superoxide dismutase activity in bovine milk serum. J. Food Protection 42:867-871.
10.4315/0362-028X-42.11.867
18
Lee, T.B. 1980. Illustrated Flora of Korea. Hyangmoonsa Publishing Co., Seoul, Korea. p. 990 (in Korea).
19
Masferrer, J., B.S. Zweifel, P.T. Manning, S.D. Hauser, K.M. Leahy, W.G. Smith, P.C. Isakson and K. Seibert. 1994. Selective inhibition of inducible cyclooxygenase 2 in vivo is anti-inflammatory and nonulcerogenic. Proc. Natl. Acad. Sci. 91:3228-3232.
10.1073/pnas.91.8.32288159730
20
McCartney-Francis, N., J.B. Allen, D.E Mizel, J.E. Albina, Q.W. Xie, C.F. Nathan and S.M. Wahl. 1993. Suppression of arthritis by an inhibitor of nitric oxide synthase. J. Exp. Med. 178:749-754.
10.1084/jem.178.2.7497688035
21
Moncada, S., R.M. Palmer and E.A. Higgs. 1991. Nitric oxide: Physiology pathophysiology, and pharmacology. Pharmacol. Rev. 43:109-114.
1852778
22
Nathan, C. 1992. Nitric ocide as a secretory product of mammalian cells. FASEB J. 6:3051-3064.
10.1096/fasebj.6.12.13816911381691
23
Nishikimi, M., N.A. Roa and K. Yagi. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine metho- sulfate and molecular oxygen. Biochem. Biophys. Res. Commun. 46:849-854.
PMC
24
Ren, K. and R. Torres. 2009. Role of interleukin-1 beta during pain and inflammation. Brain. Res. Rev. 60:57-64.
10.1016/S0006-291X(72)80218-3
25
Santos-Gomes, P.C., R.M. Seabra, P.B. Andrade and M. Fernandes- Ferreira. 2003. Determination of phenolic antioxidant compounds produced by calli and cell suspensions of sage (Salvia officinalis L.). J. Plant Physiol. 160:1025-1032.
10.1016/j.brainresrev.2008.12.02019166877PMC3076185
26
Sim, M.O., H.J Lee, J.H Jang, H.E Lee, H.K Jung, T.M Kim, J.H No, J. Jung, D.E. Jung and H.W. Cho. 2017. Anti- inflammatory and antioxidant effects of Spiraea prunifolia Sieb. et Zucc. var. simpliciflora Nakai in RAW 264.7 cells. Korean J. Plant Res. 30:335-342 (in Korean).
10.1078/0176-1617-0083114593803
27
Storck, M., M. Scgilling, K. Burkhardt, R. Prestel, D. Abendroth and C. Hammer. 1994. Production of proinflammatory cytokines and adgesion molecules in ex-vivo xenogeneic kidney perfusion. Transpl. Int. 7:S647-S649.
28
Stuehr, D.J., H.J. Cho, N.S. Kwon, M.F. Weise and C.F. Nathan. 1991. Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: An FAD-and FMN-containing flavoprotein. Proc. Natl. Acad. Sci. USA 88:7773-7777.
10.1111/j.1432-2277.1994.tb01464.x11271330
29
Talalay, P. and A.M. Benson. 1982. Elevation of quinone reductase activity by anticarcinogenic antioxidants. Advances in Enzyme Regulation 20:287-300.
10.1073/pnas.88.17.77731715579
30
Tesuka, Y., S. Irikawa, T. Kaneko, A.H. Banskota, T. Nagaoka, Q. Xionh, K. Hase and S. Kadota. 2001. Screening of chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bugeanum. J. Ethnopharmacol. 77:209-217.
10.1016/0065-2571(82)90021-8PMC
31
Wefers, H., T. Komai, P. Talalay and H. Sies. 1984. Protection against reactive oxygen species by NAD(P)H: quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Federation of European Biochemical Societies 169:63-66.
10.1016/S0378-8741(01)00300-2
32
Weisz, A., L. Cicatiello and H. Esumi. 1996. Regulation of the mouse inducible–type nitric oxid synthase gene promoter by interferon-γ, bacterial lipopolysaccharide, and NG-monomethyl- L-arginine. Biochem. J. 316:209-215.
10.1016/0014-5793(84)80290-28645207PMC1217324
33
Williams, G.M., C.X. Wang and M.J. Iatropoulos. 1990. Toxicity studies of butylated hydroxyanisole and butylated hydroxytoluene. I. Chronic feeding studies. Food Chem. Toxicol. 28:799-806.
10.1016/0278-6915(90)90052-O
34
Yun, H.Y., V.L. Dawson and T.M. Dawson. 1996. Neurobiology of nitric oxide. Crit. Rev. Neurobiol. 10:291-316.
10.1615/CritRevNeurobiol.v10.i3-4.208978984
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :4
  • Pages :303-311
  • Received Date : 2018-04-14
  • Revised Date : 2018-06-02
  • Accepted Date : 2018-06-11
  • DOI :https://doi.org/10.7732/kjpr.2018.31.4.303
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close