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2020 Vol.33, Issue 4 Preview Page

Research Article

Berchemia floribunda-mediated Proteasomal Degradation of CyclinD1 via GKS3β-dependent Threonine-286 Phosphorylation in Human Colorectal Cancer Cells

인간 대장암 세포에 대한 먹넌출 추출물의 GSK3β 의존성 threonine-286 인산화를 통한 Cyclin D1 분해

Yeongyeong Kang1
,
Hyun Ji Eo2
,
Da Som Kim1
,
Youngki Park3
,
Jeong Ho Song4
,
Gwang Hun Park5*
강 연경1
,
어 현지2
,
김 다솜1
,
박 영기3
,
송 정호4
,
박 광훈5*
1Master’s Degree Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
2Ph.D. Resercher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
3Senior Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
4Senior Researcher, Division of Special Forest Product, National Institute of Forest Science, Suwon 16631, Korea
5Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
1국립산림과학원 산림약용자원연구소, 석사연구원
2국립산림과학원 산림약용자원연구소, 박사연구원
3국립산림과학원 산림약용자원연구소, 임업연구관
4국립산림과학원 산림소득자원연구과, 임업연구관
5국립산림과학원 산림약용자원연구소, 임업연구사
* Corresponding Author
1 August 2020. pp. 271-278
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Abstract

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of the Berchemia floribunda (BF) which belongs to Rhamnaceae against human colorectal cancer cells. The treatment of BF decreased the cell proliferation in HCT116 cell and suppressed cellular accumulation of Cyclin D1 protein. Inhibition of proteasomal activity by MG132 attenuated BF-mediated Cyclin D1 downregulation and Cyclin D1 was decreased in the cell treated with BF. These findings indicates that BF-mediated Cyclin D1 downregulation may be result from Cyclin D1 proteasomal degradation. Additionally, BF-mediated Cyclin D1 degradation was blocked in the presence of LiCl, a GSK3β inhibitor, but not PD98059, SP600125, SB203580, Bay11-7082, LY294002 an ERK1/2 inhibitor, JNK inhibitor, p38 inhibitor, IκK inhibitor and PI3K inhibitor. Furthermore, BF phosphorylated Cyclin D1 at threonine-286 (Thr286), and LiCl-induced GSK3β inhibition reduced the BF mediated phosphorylation of Cyclin D1 at Thr286. These results suggested that BF may downregulate Cyclin D1 expression as a potential anti-cancer target through GSK3β dependent Cyclin D1 degradation. Therefore, this study provides that the extract of BF has anticancer activity against human colorectal cancer cells.

Keywords
Anti-cancer
Berchemia floribunda
Colorectal cancer
Cyclin D1
Proteasomal degradation
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :4
  • Pages :271-278
  • Received Date : 2020-04-13
  • Revised Date : 2020-04-29
  • Accepted Date : 2020-04-29
  • DOI :https://doi.org/10.7732/kjpr.2020.33.4.271
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