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2022 Vol.35, Issue 2 Preview Page

Research Article

Identification of 19 Species of Poisonous Plants from Jeju Island and Construction of a Database Using DNA-barcoding

DNA-barcoding을 이용한 제주도 자생 독성 식물 19종의 종 식별 및 데이터베이스 구축

Eunchae Kwon1
,
Joo-Young Kim2
,
Miwha Chang3
,
Minji Lee1
,
Seohyun Moon4
,
Won-Hae Lee4*
권 은채1
,
김 주영2
,
장 미화3
,
이 민지1
,
문 서현4
,
이 원해4*
1Rerearch Assistant, Forensic DNA Division, National Forensic Service, Wonju 26460, Korea
2Researcher, Forensic DNA Division, National Forensic Service, Wonju 26460, Korea
3Researcher, Forensic DNA Division, National Forensic Service, Wonju 26460, Korea
4Senior Researcher, Forensic DNA Division, National Forensic Service, Wonju 26460, Korea
1국립과학수사연구원 유전자과, 연구보조원
2국립과학수사연구원 유전자과, 연구사
3국립과학수사연구원 유전자과, 연구원
4국립과학수사연구원 유전자과, 연구관
*Corresponding Author
1 April 2022. pp. 346-361
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Abstract

Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.

Keywords
Forensic botany
Genome database
ITS1-ITS4
trnL-trnF
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 35
  • No :2
  • Pages :346-361
  • Received Date : 2021-12-09
  • Revised Date : 2022-03-21
  • Accepted Date : 2022-03-25
  • DOI :https://doi.org/10.7732/kjpr.2022.35.2.346
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