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2019 Vol.32, Issue 4 Preview Page

Research Article

Effect of Medium and Soil Conditions on Propagation of Gametophyte and Sporophyte in Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike

진퍼리고사리의 전엽체 및 포자체 증식에 영향을 미치는 배지 조성과 배양토의 종류

Sang In Lee1*
,
Bo Kook Jang1*
,
Cheol Hee Lee2**
이 상인1*
,
장 보국1*
,
이 철희2**
1Graduated Student, Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Korea
2Professor, Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Korea
1충북대학교 축산·원예·식품공학부 BK21플러스 생물건강소재산업화사업단, 대학원생
2충북대학교 축산·원예·식품공학부 BK21플러스 생물건강소재산업화사업단, 교수
* First Author
** Corresponding Author
31 August 2019. pp. 290-295
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Abstract

This study was carried out to select the optimal condition for prothallus propagation and sporophyte formation of Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike and to provide basic data for mass production system. In the propagation, 300 ㎎ of prothallus were inoculated in different kinds of medium [1/4, 1/2, Murashige and Skoog (MS), Knop], sucrose (0, 1, 2, 3, 4%), and cultured for 8 weeks. Sporophyte formation studies were carried out by inoculating blended prothallus into artificial soils. The soils were mixed with horticultural substrate, peatmoss, perlite, and decomposed granite at different ratios or only with the horticultural substrate. Then the prothallus was cultivated for 12 weeks to figure out the formation and growth of the sporophytes. The growth and development of prothalli were excellent on MS medium. According to medium components, prothallus growth was favorable in all treatments except 0% sucrose treatment and the highest in 2% sucrose. In the experiment of soil mixtures, sporophyte formation was the highest in the horticultural substrate:perlite 2:1 (v:v) and horticultural substrate:decomposed granite 2:1 (v:v) treatment, and the overall growth was good in the horticultural substrate:decomposed granite 2:1 (v:v) mixed soil.

Keywords
In vitro culture
Media components
Propagation
Prothallus
Soil mixture
Sporophyte
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :4
  • Pages :290-295
  • Received Date : 2019-03-16
  • Revised Date : 2019-05-22
  • Accepted Date : 2019-07-12
  • DOI :https://doi.org/10.7732/kjpr.2019.32.4.290
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