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2023 Vol.36, Issue 1 Preview Page

Research Article

Evaluation Antioxidant and Anti-inflammatory Activity of Ethanolic Extracts of Myriophyllum spicatum L. in Lipopolysaccharide-stimulated RAW 264.7 Cells

이삭물수세미(Myriophyllum spicatum L.) 에탄올 추출물의 항산화와 항염증 효과

Chul Hwan Kim12*
,
Young-Kyung Lee1
,
Min Jin Kim1
,
Ji Su Choi1
,
Buyng Su Hwang1
,
Pyo Yun Cho1
,
Young Jun Kim3
,
Yong Tae Jeong1*
김 철환12*
,
이 영경1
,
김 민진1
,
최 지수1
,
황 병수1
,
조 표연1
,
김 영준3
,
정 용태1*
1Researcher, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea
2Graduate student, Department of Food Regulatory Science, Korea University, Sejong 30019, Korea
3Professor, Department of Food Regulatory Science, Korea University, Sejong 30019, Korea
1국립낙동강생물자원관, 연구원
2고려대학교 식품규제과학과, 대학원생
3고려대학교 식품규제과학과, 교수
*Corresponding Author
†These authors contributed equally to this work.
1 February 2023. pp. 15-25
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Abstract

Myriophyllum spicatum L. has been used as an ornamental in ponds and aquariums, and as a folk remedy for inflammation and pus. Nevertheless, the biological activity and underlying mechanisms of anti-inflammatory effects are unclear. This study is aimed at investigating the antioxidative and anti-inflammatory activities of ethanol extract of Myriophyllum spicatum L. (EMS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Antioxidant activity of EMS was assessed by radical-scavenging effects on ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. As inflammatory response parameters produced by LPS-stimulated RAW 264.7 cells were quantified to assess the anti-inflammatory activity of EMS. Our results showed that EMS increased FRAP and DPPH radical- scavenging activity. In EMS-treated RAW 264.7 cells, the production of NO, PGE2, TNF-α and IL-1β was significantly inhibited at the non-cytotoxic concentration. In addition, EMS significantly attenuated LPS-stimulated the toll-like receptor (TLR) 4/myeloid differentiation protein (MyD) 88 signaling pathway, and inhibited nuclear translocation of nuclear factor-kappa B(NF-κB). Positive correlations were noted between anti-inflammatory activity and antioxidant activity. In conclusion, it was indicated that EMS suppresses the transcription of inflammatory factors by inhibiting the TLR4/MyD88/NF-κB signaling pathway, thereby suppressing LPS-stimulated inflammation in RAW 264.7 cells. This study highlights the potential role of EMS against inflammation and associated diseases.

Keywords
Anti-inflammation
Antioxidant
Cytokine
Myriophyllum spicatum L.
RAW 264.7 cells
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :1
  • Pages :15-25
  • Received Date : 2022-10-17
  • Revised Date : 2022-11-24
  • Accepted Date : 2022-11-25
  • DOI :https://doi.org/10.7732/kjpr.2023.36.1.015
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