All Issue

2019 Vol.32, Issue 5 Preview Page

Research Article

Anti-inflammatory and Anti-Atopic Effects of Crude Extracts and Solvent Fractions of Phormium tenax leaf

신서란(Phormium tenax) 잎 조추출물 및 용매 분획물의 항염증 및 항아토피 효과

Kwon Min Yang1
,
Sang mok Song2
,
Doseung Lee3
,
Weon-Jong Yoon3
,
Chan-Shick Kim4
,
Chang Sook Kim4*
양 권민1
,
송 상목2
,
이 도승3
,
윤 원종3
,
김 찬식4
,
김 창숙4*
1The Master’s Degree Researcher, Biodiversity Research Institute, Jeju Technopark, Seogwipo 63608, Korea
2The Master’s Degree Researcher, Dermapro Company, Jeju 63309, Korea
3Ph.D. Researcher, Biodiversity Research Institute, Jeju Technopark, Seogwipo 63608, Korea
4Professor, Faculty of Biotechnology, Jeju National University, Jeju 63309, Korea
1(재)제주테크노파크 생물종다양성연구소, 석사연구원
2㈜더마프로, 석사연구원
3(재)제주테크노파크 생물종다양성연구소, 박사연구원
4제주대학교 생명자원과학대학 생명공학부, 교수
* Corresponding Author
31 October 2019. pp. 433-441
PDF XML Citation
Abstract

This study describes a preliminary evaluation of the anti-inflammatory activity and anti-atopic activity of Phormium tenax leaf extracts. P. tenax leaf was extracted using 70% ethanol and then fractionated sequentially with n-hexane, methylene chloride, ethyl acetate, n-butanol. In order to effectively screen for anti-inflammatory agents, we first investigated the inhibitory effects of P. tenax leaf crude extracts and solvent fractions on production of pro-inflammatory factors[nitric oxide(NO), prostaglandin E2(PGE2), inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1β(IL-1β)] in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells. In addition, we also evaluated of their inhibitory effect on the atopic dermatitis-like inflammatory markers such as macrophage-derived chemokine(MDC) and thymus and activation-regulated chemokine(TARC) in HaCaT cells. Among the five solvent fractions of P. tenax, methylene chloride and ethyl acetate fractions inhibited production of pro-inflammatory factors and pro-inflammatory cytokines in a dose dependent manner, respectively. These fractions were also showed inhibitory activity for MDC and TARC expression levels in IFN-γ-stimulated HaCaT cells, respectively. These results suggest that P. tenax have significantly effects of anti-inflammatory activity and anti-atopic activity that might be beneficial for the topical treatment of inflammatory skin disorders.

Keywords
Anti-atopic effect
Anti-inflammatory effect
Phormium tenax
References
1
Albina, J.E. and J.S. Reichner. 1999. Nitric oxide in inflammation and immunity. New Horiz. 3:46-64.
2
Delgado, A.V., A.T. McManus and J.P. Chambers. 2003. Production of tumor necrosis factor-α, interleukin 1-β, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance Proc. Neuropeptides 37:355-361.
10.1016/j.npep.2003.09.00514698678
3
Gandhi, N.A., G. Pirozzi and N.M.H. Graham. 2017. Commonality of the IL-4/IL-13 pathway in atopic diseases. Expert Rev Clin Immunol. 13:425-437.
10.1080/1744666X.2017.129844328277826
4
Guo, S., P. Qiu, X. Wu, P. Dong, G. Yang, J. Zheng, D.J, McClements and O.H. Xia. 2012. Synergistic anti-inflammatory effects of nobiletin and sulforaphane in lipopolysaccharide- stimulated RAW 264.7 cells. J. Agri. Food. Chem. 60:2157- 2164.
10.1021/jf300129t22335189PMC3296826
5
Guttman-Yassky, E., K.E. Nograles and J.G. Kruegger. 2011. Contrasting pathogenesis of atopic drmatitis and psoriasis- part I : clinical and pathologic concepts. J. Allergy Clin. Immunol. 127:1110-1118.
10.1016/j.jaci.2011.01.05321388665
6
Guzik, T.J., R. Korbut and T. Adamek-guzik. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 54:469-487.
7
Ham, Y.M., S.H. Cho, S.M. Song, S.A. Yoon, Y.B. Lee, C.S. Kim, S.H. Kwon, M.S. Jeong, W.J. Yoon and Kim K.N. 2017. Litsenolide A2: The major anti-inflammatory activity compound in Litsea japonica fruit. J. Functional Foods. 39:168-174.
10.1016/j.jff.2017.10.024
8
Joung, M.S., C.M. Park and J.N Yoon. 2018. A novel compounds from Phormium tenax, and cosmetic composition comprising the same. Korean Intellectual Property Office, 10-1872010 (in Korean).
9
Kang, H.C., M.Y. Cha, S.J. Kim, I.Y. Baek, G.Y. Lee, M.S. Jung and M.J. Lim. 2017. lactobacillus fermentum HK and Phormium tenax fermented extract manufactured using thereof. Korean Intellectual Property Office, 10-1770140 (in Korean).
10
Kaplanski, G., V. Marin, F. Montero-Juliaan, A. Mantovani and C. Farnarier. 2003. IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol. 24:25-29.
10.1016/S1471-4906(02)00013-3
11
Kim, J.H., M.H. Kim, G. Yang, Y. Huh, S.H. Kim and W.M. Yang. 2013. Effects of topical application of Astragalus membranaceus on allergic dermatitis. Immunopharmacol Immuno-toxicol. 35:151-156.
10.3109/08923973.2012.73370823083228
12
Kim, B.Y., D.G. Bae, J.H. Kim, H.C. Lee, S.J. Jung and Y.S. Jung. 2002. Report of Ministry of Agriculture, Food and Rural Affairs. p. 22 (in Korean).
13
Kim, Y.H. and Y.S. Park. 2006. Effect of Acanthopanacis cortex water extract of antioxidantive activity, lipid profile and epidermal thickness in DNCB-induced allergic contract dermatitis animal model. Korean J. Food Sci. Technol. 38(5):668-673 (in Korean).
14
Ko, C., W.C. Hyun, J.H. Kim, Y.J. Ko, S.M. Song, M.H. Ko, J.C. Lee, C.S. Kim and W.J. Yoon. 2015. Anti-inflammatory effects on 80% ethanol extract and ethyl acetate fraction of Acrosorium yendoi Yamada in murine macrophage RAW 264.7 cells. Korean J. Plant Res. 28:574-581 (in Korean).
10.7732/kjpr.2015.28.5.574
15
Kwon, D.J., Y.S. Bae, S.M. Ju, A.R. Goh, G.S. Youn and S.Y. Choi. 2012. Causuarinin suppresses TARC/CCL17 and MDC/CCL22 production via blockade of NF-kB and STAT1 activation in HaCaT cells. Biochem. Bioph. Res. Co. 417:1254-1259.
10.1016/j.bbrc.2011.12.11922227193
16
Lazarov, S., M. Balutsov and E. Ianev. 2000. The role of bacterial endotoxins, receptors and cutokines in the pathogenesis of septic (endotoxin) shock. Vutr. Boles. 32:33-40.
17
Lee, J.S., I.S. Kim, J.H. Kim, J.S. Kim, D.H. Kim and C.Y. Yun. 2008. Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune responses. J. Ethnopharmacol. 117:34-40.
10.1016/j.jep.2008.01.01318325701
18
Moeslinger, T., R. Friedl and P.G. Spieckermann. 2006. Inhibition of inducible nitric oxide synthesis by azathioprine in a mcrophage cell line. Life Sci. 79:374-381.
10.1016/j.lfs.2006.01.01516473374
19
Riley, M. 1994. Maori healing and herval. An ehtnobotanical sourcebook. Paraparaumu, New Zealand, Viking Sevenseas.
20
Shaw, T.E., G.P. Currie, C.W, Koudelka and E.L. Simpson. 2011. Eczema prevalence in the united state: data from the 2003 national survey of children's health. J. Invest Dermatol. 131:67-73.
10.1038/jid.2010.25120739951PMC3130508
21
Soumelis, V., P.A. Reche and H. kanzler. 2002. Human epithelial cell trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat. Immunol. 3:673-680.
10.1038/ni80512055625
22
Van Snick, J. 1990. Interleukin-6: An Overview. Annu. Rev. Immunol. 8:253-278.
10.1146/annurev.iy.08.040190.0013452188664
23
Woo, H.S., S.M. Lee, J.D. Heo, M.S. Lee, Y.S. Kim and D.W. Kim. 2018. Anti-inflammatory activity of extracts of Hovenia dulcis on lipopolysaccharides-stimulated RAW264.7 cells. Korean J. Plant Res. 31:466-477 (in Korean).
24
Yoon, W.J., M.S. Song, Y.M Ham, D.J. Oh, C.S. Ko, S.A. Yoon, Y.B. Lee, D.W. Park, Y.J. Jeong, J.E. Kwon, Y.M. Cho, J.H. Cho, C.S. Kim and S.C. Kang. 2015. Anti- osteoarthritis effects on fruit extract of Litsea japonica. Korean J. Plant Res. 28:591-599 (in Korean).
10.7732/kjpr.2015.28.5.591
25
Zamora, R., Y. Vodovotz and T.R. Billiar. 2000. Inducible nitric oxide synthase and inflammatory diseases. Mol. Med. 6:347-373.
10.1007/BF0340178110952018PMC1949959
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :5
  • Pages :433-441
  • Received Date : 2019-05-30
  • Revised Date : 2019-09-11
  • Accepted Date : 2019-09-27
  • DOI :https://doi.org/10.7732/kjpr.2019.32.5.433
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close