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2022 Vol.35, Issue 2 Preview Page

Research Article

Effect of Seed Moisture Content on Seed Storage of Dehisced Ginseng Seeds

종자 수분함량에 따른 개갑 인삼 종자의 저장성 연구

Su Jeoung Suh1*
,
Jin Yu2
,
In-Bae Jang2
,
Young-Chang Kim3
서 수정1*
,
유 진2
,
장 인배2
,
김 영창3
1Post-doc., Department of Herbal Crop Research, NIHHS, RDA, Eumseong 27709, Korea
2Researcher, Department of Herbal Crop Research, NIHHS, RDA, Eumseong 27709, Korea
3Senior Researcher, Department of Herbal Crop Research, NIHHS, RDA, Eumseong 27709, Korea
1농촌진흥청 국립원예특작과학원 인삼특작부, 박사후연구원
2농촌진흥청 국립원예특작과학원 인삼특작부, 연구사
3농촌진흥청 국립원예특작과학원 인삼특작부, 연구관
*Corresponding Author
1 April 2022. pp. 183-191
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Abstract

Ginseng (Panax ginseng C.A. Meyer) is a perennial plant and propagates by seeds, and those need after-ripening for germination. To be ready for climate change and to ensure a stable seed supply, the technique for storing seeds in short-term and long-term in large quantities is required. In this study, dehisced ginseng seeds from two locations, batch #1 and batch #2, were stored at -3.5℃ with different moisture content, and after 3, 15, and 27 months of storage, the percentage of radicle emergence and shoot emergence were measured. After 3 months, radicle emergence and shoot emergence were normal only when the seed moisture content was more than 35%, and overall, germination was higher in batch #2 than in batch #1. After 15 months, the partially dehydrated seeds, with a moisture content between 45 to 54%, showed the highest germination rates, and most of the undried seeds were spoiled and failed to germinate. Seeds with moisture content lower than 25% had poor germination, too. The partially dehydrated seeds also succeeded in germination and growth in the soil after 15 months of storage, but deteriorated after one more 1 year, too. In summary, ginseng seeds look like have temperate recalcitrant seed characteristics, and partial dehydration allows extension of seed longevity.

Keywords
Dehydration
Germination
Panax ginseng C.A. Meyer
Seeds
Storage
References
1
Baskin, C.C. and J.M. Baskin. 2014. Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination, 2nd ed. Academic Press, CA (USA). pp. 119-143. 10.1016/B978-0-12-416677-6.00005-6
2
Bonner, F.T. 1990. Storage of seeds: Potential and limitations for germplasm conservation. Forest Ecol. Manag. 35:35-43. 10.1016/0378-1127(90)90230-9
3
Choi, C.H., W.S. Tak and K.J. Cho. 2006. Effect of priming treatment using PEG on seed germination in Acer mono Max. Korean J. Plant Res. 19:480-487 (in Korean).
4
Connor, K.F. and F.T. Bonner. 1999. Effects of Temperature and Moisture Content on the Storability of Hardwoods Seeds: In Haywood, J.D. (ed.), Tenth Biennial Southern Silvicultural Research Conference. USDA Forest Service, Southern Research Station. MS (USA). pp. 123-126.
5
Derkx, M.P.M. 2000. Pre-treatment at controlled seed moisture content as an effective means to break dormancy in tree seeds: In Vimont, J.D. and J. Grabbe (eds), Dormancy in Plants: From whole plant behaviour to cellular control. CABI, Wallingford, UK. pp. 68-85.
6
Ellis, R.H., T.F. Hong and E.G. Roberts. 1990. An intermediate category of seed storage behaviour? I. Coffee. J. Exp. Bot. 41:1167-1174. 10.1093/jxb/41.9.1167
7
Gosling, P.G., Y. Samuel and A. Peace. 2003. The effect of moisture content and prechill duration on dormancy breakage of Douglas fir seeds (Pseudotsuga menziesii var. menziesii [Mirb.] Franco). Seed Sci. Res. 13:239-246. 10.1079/SSR2003141
8
Ha, Y.I., J.M. Lim, S.M. Ko, J.R. Liu and D.W. Choi. 2006. Sequence variability and expression characteristics of the ginseng (Panax ginseng C.A. Meyer) dehydrin gene family. J. Plant Biol. 49:205-211. 10.1007/BF03030534
9
Han, E.J., E. Popova, G.T. Cho, S.U. Park, S.C. Lee, H.W. Pritchard and H.H. Kim. 2016. Post-harvest embryo development in ginseng seeds increases desiccation sensitivity and narrows the hydration window for cryopreservation. Cryo-Lett. 37:284-294.
10
Jones, S.K. and P.G. Gosling. 1994. “Target moisture content” prechill overcomes the dormancy of temperate conifer seeds. New Forest 8:309-321.
11
Kim, D.H., S.H. Han, J.H. Song and K.G. Jang. 2012. Seed storage and longevity in woody plant. Korea Forest Research Institute, Seoul, Korea. pp. 49-118 (in Korean).
12
Kim, H.H., J.H. Lee, D.J. Shin, H.C. Ko, H.S. Hwang, T. Kim, E.G. Cho and F. Engelmann. 2008. Desiccation sensitivity and cryopreservation of Korean ginseng seeds. Cryo-Lett. 229:419-426.
13
Kim, Y.C., Y.B. Kim, I.H. Jo, J.U. Kim, J.W. Lee, K.H. Bang, D.H. Kim and K.H. Kim. 2015a. Stratification characteristics of ginseng seed by storage period (Panax ginseng C.A. Meyer). Proceedings of Symposium. May 2015. Korean J. Medicinal Crop. Sci. pp. 119-120 (in Korean).
14
Kim, Y.C., Y.B. Kim, J.U. Kim, J.W. Lee, I.H. Jo, K.H. Bang, D.H. Kim and K.H. Kim. 2015b. Difference in growth characteristics of 5-year-old ginseng grown by direct seeding and transplanting. Korean J. Medicinal Crop. Sci. 23:480-488 (in Korean). 10.7783/KJMCS.2015.23.6.480
15
Kleinwächter, M., A. Radwan, M. Hara and D. Selmar. 2014. Dehydrin expression in seeds: an issue of maturation drying. Front. Plant Sci. 5:402. 10.3389/fpls.2014.0040225221559PMC4145252
16
Kwon, W.S., C.M. Jung, S.D. Ahn and K.T. Choi. 1986. Effects of growth regulators on the germination of Panax ginseng C.A. Meyer. J. Ginseng Res. 10:159-166 (in Korean).
17
Kwon, W.S., J.H. Lee and M.G. Lee. 2001. Optimum chilling terms for germination of the dehisced ginseng (Panax ginseng C.A. Meyer) seed. J. Ginseng Res. 25:167-170 (in Korean).
18
Lee, E.K., C.H. Bae, Y.J. Kim, S.D. Park, J.J. Shim, Y. Yu and J.L. Lee. 2021. Bioavailability and anti-inflammatory effect of fermented red ginseng in BALB/c mouse. Korean J. Plant Res. 34:433-442 (in Korean).
19
Lee, J.C. and J.T.A. Proctor. 1996. Low temperature tolerance of Panax quinquefolium. J. Ginseng Res. 20:179-183.
20
Lee, J.C., J.S. Byen and J.T.A. Proctor. 1986. Dormancy of ginseng seed as influenced by temperature and gibberellic acids. Korean. J. Crop Sci. 31:220-225.
21
Lee, J.H., S.S. Lee, I.O. Ahn, J.Y. Kang and M.G. Lee. 2004. Relationship between storage periods and germination ability of dehisced seeds of Panax ginseng C.A. Meyer. J. Ginseng Res. 28:215-218 (in Korean). 10.5142/JGR.2004.28.4.215
22
Lee, J.W., Y.C. Kim, J.U. Kim, I.H. Jo, H.K. Kim and D.H. Kim. 2016. Effects of gibberellic acid and alternating temperature on breaking seed dormancy of Panax ginseng C.A. Meyer. Korean J. Medicinal Crop Sci. 24:284-293 (in Korean). 10.7783/KJMCS.2016.24.4.284
23
Noland, T.L., A.E. Morneault, D.C. Dey and D. Deugo. 2013. The effect of storage temperature and duration on northern red oak acorn viability and vigour. Forest. Chron. 89:769-776. 10.5558/tfc2013-139
24
O’Reilly, C. and N. de Atrip. 2007. Seed moisture content during chilling and heat stress effects after chilling on the germination of common alder and downy birch seeds. Silva Fenn. 41:235-246. 10.14214/sf.293
25
Park, H.B., C.H. Ko, S.Y. Kim, K.C. Lee, J.H. Kim and J.M. Chung. 2019. Dormancy type and germination characteristics of seeds of Lonicera chrytsantha Turcz. Ex Ledeb (Caprifoliaceae). Korean J. Plant Res. 32:457-462 (in Korean).
26
Rajametov, S., Y.Y. Lee, Y.C. Kim, S. Lee, J.Y. Yi, Y.A. Jeon, J.S. Sung, G.A. Lee and J.G. Gwak. 2014. Response of pre and post treatments for cryopreservation of Korean ginseng seeds on recovering viability. Korean J. Breeding Sci. 46:408-416. 10.9787/KJBS.2014.46.4.408
27
RDA (Rural Development Administration). 2014. Ginseng. RDA. Jeonju, Korea.
28
Suh, S.J., I.B. Jang, I.B. Jang, J.W. Moon and J. Yu. 2019. Optimum storage temperature for spring sowing of Panax ginseng seeds. Korean J. Medicinal Crop Sci. 27:390-396 (in Korean). 10.7783/KJMCS.2019.27.6.390
29
Suh, S.J., I.B. Jang, J. Yu, I.B. Jang, H.W. Park, T.S. Seo and K.B. Kweon. 2017. Effect of seed dehydration and temperature during cold-stratification on the seed quality of Panax ginseng C.A. Meyer. Korean J. Medicinal Crop Sci. 25:209-216 (in Korean). 10.7783/KJMCS.2017.25.4.209
30
Yoon, J.W., H.H. Kim, J.H. Lee, J.K. Choi, S.S. Lee, Y.M. Choi and T.S. Kim. 2005. Optimal desiccation condition and moisture content of dehisced seeds of ginseng (Panax ginseng C.A. Meyer) for cryopreservation. Korean J. Crop Sci. 50:406-410 (in Korean).
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 35
  • No :2
  • Pages :183-191
  • Received Date : 2022-01-13
  • Revised Date : 2022-03-08
  • Accepted Date : 2022-03-09
  • DOI :https://doi.org/10.7732/kjpr.2022.35.2.183
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