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2018 Vol.31, Issue 4 Preview Page

Research Article

Antimicrobial Activity Screening of Sixty-four Evergreen Woody Species According to Extraction Conditions against Trichophyton mentagrophytes

상록성 목본 64종의 추출조건에 따른 무좀원인균의 항균활성 스크리닝

Bo Kook Jang1
,
Lai Won Chi1
,
Ju Sung Cho2
,
Cheol Hee Lee1*
장 보국1
,
지 래원1
,
조 주성2
,
이 철희1*
1Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University
2Quality Control & Seed Tech Team, Nongwoo Bio CO., LTD.
1충북대학교 축산 ‧ 원예 ‧ 식품공학부 생물건강소재산업화사업단문소속
2㈜농우바이오 품질관리기술팀
* Corresponding Author
31 August 2018. pp. 330-341
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Abstract

This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete’s foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 ㎜, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 ㎜. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 ㎜ (45 minutes ethanolic extraction), 20.5 ㎜ (45 minutes crude methanolic extraction) and 19.7 ㎜ (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete’s foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.

Keywords
Athlete’s foot
Methylparaben
Phenoxyethanol
Pruning product
Ultrasonic extraction
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :4
  • Pages :330-341
  • Received Date : 2018-03-19
  • Revised Date : 2018-05-17
  • Accepted Date : 2018-05-28
  • DOI :https://doi.org/10.7732/kjpr.2018.31.4.330
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