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2018 Vol.31, Issue 4 Preview Page

Research Article

Inhibitory Effects of Ethanol Extract from Angelica tenuissima Root on Oxidative Stress and Melanogenesis
Hyun Jung Koo1*
,
Sung Ryul Lee2*
,
Yuna Park3
,
Jin Woo Lee3
,
Gyeongseop So3
,
Sung Hyeok Kim3
,
Chang Woo Ha3
,
Sang Eun Lee3
,
Jong Phil Bak3
,
Su Ryeon Ham4
,
Hyosun Lim4
,
Youn Kyu Kim4
,
Eun-Hwa Sohn4**
1Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries
2Department of Convergence Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine
3Department of Herbal Medicine Resource, Kangwon National University
4Korea Research Institute Bio Science Co., Ltd.
*First Author
**Corresponding Author
31 August 2018. pp. 312-321
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Abstract

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at 80°C (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were 5.52±0.07 ㎍ quercetin equivalents and 237.27±13.24 ㎍ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to 1000 ㎍/㎖. The amount of melanin synthesis followed by α-melanocyte stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to 1000 ㎍/㎖, p<0.05). ATE (125 to 1000 ㎍/㎖, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on α-glucosidase activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

Keywords
Angelica tenuissima Nakai
Anti-melanogenic
Anti-oxidant
Decursin
α-Glucosidase
Melanin
Tyrosinase
Z-Ligustilide
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :4
  • Pages :312-321
  • Received Date : 2018-06-01
  • Revised Date : 2018-06-25
  • Accepted Date : 2018-07-06
  • DOI :https://doi.org/10.7732/kjpr.2018.31.4.312
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