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2018 Vol.31, Issue 4 Preview Page

Research Article

Anti-inflammatory Effect of the Robinia pseudoacacia L. High Temperature Extract

아까시 나무 고온추출물의 항염증 효과

Jong Hyun Nho1
,
Byoung Man Kang1
,
Won Seok Jung1*
노 종현1
,
강 병만1
,
정 원석1*
1National Development Institute of Korean Medicine
1한약진흥재단
* Corresponding Author
31 August 2018. pp. 294-302
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Abstract

This study was conducted to compare anti-inflammatory effect of Robinia pseudoacacia L. using different extraction methods (water extraction, ethanol extraction and high temperature extraction). We investigated anti-inflammatory effect of Robinia pseudoacacia L. extract (RP1, water extract; RP2, ethanol extract; RP3, high temperature extract) on lipopolysaccharide (LPS)-stimulated inflammation using Raw 264.7 cell. Cells were treated with various concentrations (12.5, 25, 50, 100 or 200 ㎍/㎖) of water extract, ethanol extract and high temperature extract. Cytotoxicity was not observed on Raw 264.7 cells, LPS-stimulated production of NO (nitric oxide), PGE2 (prostaglandin E2) and cytokines (TNF-α, IL-6 and IL-1β) was reduced by RP3 treatment more than RP1 and RP2. In conclusion, these results indicated that inflammation on Raw 264.7 cells was improved by RP3. Treatment of RP3 could be used to natural medicine for improving inflammatory response. However, further experiment is required to observe how the high temperature extraction at 500℃ for 48 h influences on alteration of active ingredient in Robinia pseudoacacia L., and conducts the inflammation signal pathway on Raw 264.7 cells.

Keywords
Extraction
Inflammation
Raw 264.7 cells
Robinia pseudoacacia L.
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :4
  • Pages :294-302
  • Received Date : 2018-04-05
  • Revised Date : 2018-06-20
  • Accepted Date : 2018-07-14
  • DOI :https://doi.org/10.7732/kjpr.2018.31.4.294
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