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2020 Vol.33, Issue 6 Preview Page

Research Article

Isolation of New Strain of Cordyceps militaris HB8 and Optimal Condition for Production of Adenosine and Cordycepin in Fruit Body
Jin Feng Li1†
,
Van An Hoang2†
,
Jong Chan Ahn3
,
Dong Uk Yang4
,
Dong Wook Lee5
,
Deok Chun Yang6†
1Graduate Student, Department of Oriental Medicinal Biotechnology, Kyung Hee University, Yongin 17104, Korea
2Researcher, HanbangBio Inc. Kyung Hee University Holdings, Yongin 16954, Korea
3Graduate Student, Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea
4Manager, HanbangBio Inc. Kyung Hee University Holdings, Yongin 16954, Korea
5Research Director, HanbangBio Inc. Kyung Hee University Holdings, Yongin 16954, Korea
6CEO, HanbangBio Inc. Kyung Hee University Holdings, Yongin 16954, Korea, Professor, Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea
*dcyang@khu.ac.kr
†These authors contributed equally to this work.
1 December 2020. pp. 696-706
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Abstract

Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 ㎎/g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 ㎎/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

Keywords
Adenosine
Cordycepin
Cordyceps militaris
Fruiting-body
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :6
  • Pages :696-706
  • Received Date : 2020-11-03
  • Revised Date : 2020-11-24
  • Accepted Date : 2020-11-26
  • DOI :https://doi.org/10.7732/kjpr.2020.33.6.696
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