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2018 Vol.31, Issue 6 Preview Page

Research Article

Analysis of Scutellaria baicaleinsis Georgi (Scutellariae Radix) by LC-DAD and LC-ESI/MS
Youngbob Yu1*
,
Pil-Son Choi1
,
Sungtae Koo2
,
Suhwan Chang3
1Professor, Department of Herbal Pharmaceutical Development & Department of Emergency Medical Rescue, Nambu University
2Professor, Department of Korean Medical Science, School of Korean Medicine, Pusan National University
3Professor, Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center
*Corresponding Author
31 December 2018. pp. 652-659
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Abstract

In this study, baicalin, as a marker substance of Scutellariae Radix, was quantitatively analyzed by a high performance liquid chromatography-photodiode array detector (HPLC-DAD). We identified wogonoside, baicalein, and wogonin in the Scutellariae Radix by a high performance liquid chromatography-electrospray ionization-mass spectrometer (HPLC-ESI-MS). The baicalin was separated on a Xterra C18 column (5 ㎛, 4.6 x 250 ㎜) using mobile phase consisting of 38% acetonitrile in 0.68% phosphoric acid. The baicalin spectrum in the Scutellariae Radix extracts was coincided by comparing with UV-visible spectrum (200-550 ㎚) of baicalin standard in the library. The amount of baicalin in Scutellariae Radix was 10.46%, which is higher than KFDA’s guideline. The marker substances of Scutellariae Radix showed a strong base peak [M]+ in the positive detection mode following as; baicalin (m/z; 271 [MH+-sugar]+, 447 [M+H]+), wogonoside (m/z; 285 [MH+-sugar]+, 461 [M+H]+), baicalein (m/z; 271 [M+H]+), wogonin (m/z; 285 [M+H]+). These results are consistent with the fragment pattern and molecular weight of standard components from literature.

Keywords
4 marker substances
LC-DAD
LC-ESI/MS
Scutellariae Radix
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :652-659
  • Received Date : 2018-11-22
  • Accepted Date : 2018-12-18
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.652
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