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2021 Vol.34, Issue 1

Research Article

Phylogenetic Analysis on Wild Cordyceps Collected from Miryang Region of South Korea

밀양근교에서 채집한 야생 동충하초 계통의 PCR 산물에 근거한 계통 유전학적 연구

Hyeancheal Park1†
,
Sangmong Lee1
,
Namsook Park2
박 현철1†
,
이 상몽1
,
박 남숙2
1Professor, Department of Life Science & Environmental Biochemistry, Pusan National University, Miryang 50463, Korea
2Researcher, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea
1부산대학교 생명환경화학과, 교수
2부산대학교 생명산업융합연구원, 연구원
*hcpark@pusan.ac.kr
1 February 2021. pp. 1-16
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Abstract

The phylogenetic relationships among thirty-two strains (P1∼P32; including Cordyceps sp., Paecilomyces sp., Beauveria sp., Aranthomyces sp., Isaria sp. and Himenostilbe sp.) in Miryang region located in the southern part of Korea, were investigated based on internal transcribed spacer (ITS) sequences of ribosomal DNA. A fragment of ITS region was amplified by polymerase chain reaction (PCR) using the specific primer pairs ITS1 and ITS4. After obtained same size of PCR products from various strains, we cloned them into a pGEM-T easy vector to determine their sequences. BLAST analyses of the nucleotide sequence ITS1, 5.8S and ITS2 gene fragments revealed the identity and their phylogenetic relationship. Among 32 strains isolated from Miryang region, Cordyceps militaris was shared 100% sequences with Genbank (AY49191, EU825999, AY491992), while some species are not shared perfectly with reported sequences. For example, strain P17 (P. tenuipes in Ulju-gun Gaji Mountain) has some differences among the other strains of P. tenuipes (Miryang-si Jocheon-eup, Miryang-si Gaji Mountain) and those of gene bank. We conclude that ITS analyses with strains in the suburbs of Miryang in this study can be effectively used as a tool for classification, evaluation and collection of the natural eco-type genetic resources.

Keywords
Cordyceps sp.
Genetic resource
Internal transcribed spacer
Phylogenetic analysis
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :1
  • Pages :1-16
  • Received Date : 2020-10-05
  • Revised Date : 2020-12-06
  • Accepted Date : 2020-12-08
  • DOI :https://doi.org/10.7732/kjpr.2021.34.1.001
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