Enhancing in vitro Growth of Bulbs for Mass Propagation of Lily Germplasm

Jae-young Song; Young-yi Lee; Jung-yoon Yi; Jung-ro Lee; Mun-sup Yoon

doi:10.7732/kjpr.2020.34.1.017

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2021 Vol.34, Issue 1 Preview Page Next Page

Research Article

Enhancing in vitro Growth of Bulbs for Mass Propagation of Lily Germplasm

1 February 2021. pp. 17-22

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Abstract

Plants regenerated from in vitro cultures carry chromosomal variations, especially in long-term culture. Reducing the duration of plant tissue culture is one of the ways to reduce genetic and epigenetic changes. In this study, we reduced the duration of long-term culture and repeat subculture using small bulblets derived from bulb scales in two lily cultivars. The adventitious bulblets derived from bulb-scale tissue were cultured on three different media containing Murashige and Skoog (MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 ㎎/L IAA and 0.4 ㎎/L BA hormone with or without Charcoal, respectively. About seven weeks later, the number of newly propagated multiple shoots in the two media, A and B media, showed little differentiation. Compared to both media, the number of propagated multiple shoots increased 5-fold in MS medium containing 0.3 ㎎/L IAA and 0.4 ㎎/L BA hormone without Charcoal (C medium). The number of propagated multiple shoots ranged from 5 to 6 and 4 to 6 with an average of 5 in TropicalPink and GreenStar cultivars, respectively. The flow cytometric measurements indicated no variation in the ploidy level between control and in vitro propagated plants.

Keywords

Activated charcoal

Bulb

Lilium

Ploidy

Propagation

References

Ahuja, A. 1985. In vitro shoot differentiation in Eucalyptus citriodora Hook: effect of activated charcoal. Ind. J. F. 8:340-341.

Amasino, R.D. and C.O. Miller. 1982. Hormonal control of tobacco crown gall tumor morphology. Plant Physiol. 69:389-392. 10.1104/pp.69.2.38916662215PMC426216

Askari, N., Y.G. Wang and G.J. De Klerk. 2014. In tissue culture of Lilium explants may become heavily contaminated by the standard initiation procedure. Propag. Ornam. Plants 14:49-56.

Boggetti, B., J. Jasik and S.H. Mantell. 1999. In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plants. Plant Cell Rep. 18:456-461. 10.1007/s002990050603

Chen, X.L., J.H. Li, X. Xin, Z.E. Zhang, P.P. Xin and X.X. Lu. 2011. Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification. S. Afr. J. Bot. 77(2):397-403. 10.1016/j.sajb.2010.10.005

Ebert, A., F. Taylor and J. Blake. 1993. Changes of 6-benzylaminopurine and 2,4-dichiorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tissue Organ Cult. 33:157-163. 10.1007/BF01983229

Fridborg, G., M. Pedersen, N. Lanstrom and T. Eriksson. 1978. The effect of activated charcoal on tissue cultures: Adsorption of metabolites inhibiting morphogenesis. Physiol. Plant. 43:104-106. 10.1111/j.1399-3054.1978.tb01575.x

Fridborg, G. and T. Erikson. 1975. Effect of activated charcoal on growth and morphogenesis in cell cultures. Physiol. Plant. 34:306-308. 10.1111/j.1399-3054.1975.tb03843.x

George, E.F. and P.D. Sherrington. 1984. Plant propagation by tissue culture - Handbook and Dictionary of commercial Laboratories. Exegetics Limited, Eversley, Basingstoke, Hants, UK.

Guri, A.Z. and K.N. Patel. 1998. Compositions and methods to prevent microbial contamination of plant tissue culture media. United States Patent 5(750):402.

Halmagyi, A, G., G. Fischer-Kluver, G. Mix-Wanger and H.M. Schumacher. 2004. Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches. Plant Cell Rep. 22:371-375. 10.1007/s00299-003-0703-913680136

Hartmann, H.T., D.E. Kester, F.T. Davies and R.L. Geneve. 2011. Hartmann & Kester's Plant Propagation: Principles and Practices. 8th ed., Pearson Education, Ltd., Pearson Prentice Hall, USA.

Joshi, S.K. and U. Dhar. 2009. In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high altitude Himalaya. Acta Physiol. Plant. 31:833-838. 10.1007/s11738-009-0299-y

Komalavalli, N. and M.V. Rao. 2000. In vitro micropropagation of Gymnema sylvestre - A multipurpose medicinal plant. Plant Cell Tissue Organ Cult. 61:97-105. 10.1023/A:1006421228598

Larkin, P. and W.R. Scowcroft. 1981. Somaclonal variation - A novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197-214. 10.1007/BF0234254024276737

Liu, J., J. Zhang, B. Xu, C. Jia, J. Zhang, G. Tan and Z. Jin. 2011. Regeneration and production of transgenic Lilium longiflorum via Agrobacterium tumefaciens. In Vitro Cell. Dev. Biol.-Plant 47:348-356. 10.1007/s11627-010-9336-1

Naing, A.H., S.H. Kim, M.Y. Chung, S.K. Park and C.K. Kim. 2019. In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars. Plant Methods 15:36. 10.1186/s13007-019-0421-031011361PMC6461810

Ozel, C.A., K.M. Khawar and F. Unal. 2015. Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale. Saudi J. Biol. Sci. 22:132-138. 10.1016/j.sjbs.2014.09.00725737643PMC4336438

Paek, K.Y., K.J. Yu and S.I. Park. 1998. In vitro propagation by shoot-tip and node-bud culture of Rehmannia glutinosa. Korean J. Plant Tissue Culture 25:63-68.

Scowcroft, W.R. 1984. Genetic Variability in Tissue Culture: Impact on Germplasm Conservation and Utilization. International Board for Plant Genetic Resources, Report No: AGPG: IBPGR/84/152, IBPGR, Rome, Italy.

Song, J.Y., J.Y. Yi, M.Y. Yoon, J.R. Lee and Y.Y. Lee. 2019. An efficient In vitro micropropagation for production of disease-free bulbs in Korean native Lilium. Korean J. Plant Res. 32:730-734.

Steimitz, B. and H. Yahel. 1982. In vitro propagation of Narcissus tazetta. HortScience 17:333-334.

Wang, P.J. and L.C. Huang. 1976. Beneficial effects of activated charcoal on plant tissue and organ cultures. In vitro 12:260-262. 10.1007/BF027964471262045

Wang, Q.C., R. Gafny, N. Sahar, I. Sela, M. Mawassi, E. Tanne and A. Perl. 2002. Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration. Plant Sci. 162:551-558. 10.1016/S0168-9452(01)00594-5

Wang, Q.C. and A. Perl. 2006. Cryopreservation in Floricultural plants: In Teixeira de Silva, J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Global Science Book, London, UK. pp. 524-539.

Xia, Y., X. Deng, P. Zhou, K. Shima and J. Teixeira da Silva. 2006. The world floriculture industry: Dynamics of production and markets: In: J.A. Teixeira da Silva, (ed.), Floriculture, Ornamental and Plant Biotechnology, Advances and Topical Issues, Volume IV, Global Science Books London, UK. pp. 336-347.

Yang, D.C., C.K. Kang and K.T. Choi. 1992. Effects of phytohormone and activated carbon on the growth and rooting of teratoma shoot induced from crown gall callus in Nicotiana tabacum cv. NC2326. Journal of the Korean Society of Tobacco Science 14:33-41.

Youssef, N.M., S.A. Shaaban, Z.F. Ghareeb and L.S. Taha, 2019. In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. "Starfighter" plants. Bull. Nati. Res. Cent. 43(1):211. 10.1186/s42269-019-0246-z

Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 34
No :1
Pages :17-22
Received Date : 2020-11-03
Revised Date : 2020-12-01
Accepted Date : 2020-12-01
DOI :https://doi.org/10.7732/kjpr.2020.34.1.017

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

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