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2018 Vol.31, Issue 6 Preview Page
December 2018. pp. 597-603
Abstract

Inhibitory effect of colchicine on growth and gravitropic responses in Arabidopsis root was explored to find whether there was an involvement of ethylene production. It has been known that cytoskeleton components are implicated in sedimentation of statoliths to respond to gravitropism and growth. The root growth was inhibited by 25% and 40% over control for 8 hr treatment of colchicine at a concentration of 10-5 M and 10-7 M, respectively. The roots treated with colchicine at the concentration of 10-7 M showed the same pattern as control in 3 hr, however, gravitropic response was decreased in the next 5 hr. The colchicine treatment at the concentration of 10-5 M inhibited the gravitropic response resulting in 60° of curvature. In order to better understand the role of colchicine, the production of ethylene was measured with and without the treatment of colchicine. Colchicine increased the ethylene production by 20% when compared to control via the activation of ACC oxidase and ACC synthase activity. These results suggest that the inhibition of the growth and gravitropic responses of Arabidopsis roots by the treatment of colchicine could be attributed to the rearrangement of microtubule, and increase of ethylene production.

References
  1. Baskin, T.I., G.T.S. Beemster, J.E. Judy-March and F. Marga. 2004. Disorganization of cortical microtubules stimulates tangential expansion and reduces the uniformity of cellulose microfibril alignment among cells in the root of Arabidopsis. Plant Physiol. 135:2279-2290.10.1104/pp.104.04049315299138PMC520797
  2. Baskin, T.I., H.T.H.M. Meekes, B.M. Liang and R.E. Sharp. 1999. Regulation of growth anisotropy in well-watered and water-stressed maize roots. II. role of cortical microtubules and cellulose microfibrils. Plant Physiol. 119:681-692.10.1104/pp.119.2.6819952465PMC32146
  3. Blancaflor, E.B. and P.H. Masson. 2003. Plant gravitropism. Unraveling the ups and downs of a complex process. Plant Physiol. 133:1677-1690.10.1104/pp.103.03216914681531PMC1540347
  4. Bleecker, A.B. and H. Kende. 2000. Ethylene: a gaseous signal molecule in plants. Ann. Rev. Cell Dev. Bi. 16:1-18.10.1146/annurev.cellbio.16.1.111031228
  5. Chan, J. 2012. Microtubule and cellulose microfibril orientation during plant cell and organ growth. J. Microscopy 247:23-32.10.1111/j.1365-2818.2011.03585.x22171640
  6. Chen, R., E. Rosen and P.H. Masson. 1999. Gravitropism in higher plants. Plant Physiol. 120:343-350.10.1104/pp.120.2.34311541950PMC1539215
  7. Durnam, D.J. and R.L. Jones. 1982. The effects of colchicine and gibberellic acid on growth and microtubules in excised lettuce hypocotyls. Planta 154:204-211.10.1007/BF0038786524276062
  8. Emons, A.M.C., A.M.C. Wolters-Arts, J.A. Traas and J. Derksen. 1990. The effect of colchicine on microtubules and microfibrils in root hairs. Acta. Bot. Neerl. 39:19-27.10.1111/j.1438-8677.1990.tb01442.x
  9. Hou, G., D.R. Mohamalawari and E.B. Blancaflor. 2003. Enhanced gravitropism of roots with a disrupted cap actin cytoskeleton. Plant Physiol. 131:1360-1373.10.1104/pp.01442312644685PMC166895
  10. Kim, S.Y., Y.K. Kim, K.S. Kwon and K.W. Kim. 2000. Action of malformin A1 on gravitropic curvature in primary roots of maize (Zea mays L.). J. Plant Biol. 43:183-188.10.1007/BF03030417
  11. Lee, J.S., W.K. Chang and M.L. Evans. 1990. Effects of ethylene on the kinetics of curvature and auxin redistribution in gravistimulated roots of Zea mays. Plant Physiol. 94:1770-1775.10.1104/pp.94.4.177011537475PMC1077451
  12. Ma, K.B., K.S. Cho, H.W. Jung, H.J. Seo and S.S. Kang. 2018. Induction of a chromosome-doubled persimmon (Diospyros kaki Thunb.) by in vitro colchicine treatment. Korean J. Plant Res. 31:515-521.
  13. Ma, Q., X. Wang, J. Sun and T. Mao. 2018. Coordinated regulation of hypocotyl cell elongation by light and ethylene through a microtubule destabilizing protein. Plant Physiol. 176:678-690.10.1104/pp.17.0110929167353PMC5761786
  14. Ma, Z. and Y. Ren. 2012. Ethylene interacts with auxin in regulating developmental attenuation of gravitropism in flax root. J. Plant Growth Regul. 31:509-518.10.1007/s00344-012-9261-0
  15. Mekhedov, S.L. and H. Kende. 1996. Submergence enhances expression of a gene encoding 1-aminocyclopropane-1-carboxylate oxidase in deepwater rice. Plant Cell Physiol. 37:531-537.10.1093/oxfordjournals.pcp.a0289768759917
  16. Planchais, S., N. Glab, D. Inze and C. Bergounioux. 2000. Chemical inhibitors: a tool for plant cell cycle studies. FEBS Letters 476:78-83.10.1016/S0014-5793(00)01675-6
  17. Ruzicka, K., K. Ljung, S. Vanneste, R. Podhorska, T. Beeckman, J. Friml and E. Benkova. 2007. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution. Plant Cell 19:2197-2212.10.1105/tpc.107.05212617630274PMC1955700
  18. Santisree, P., S. Nongmaithem, Y. Sreelakshmi, M. Ivanchenko and R. Sharma. 2012. The root as a drill: An ethylene-auxin interaction facilitates root penetration in soil. Plant Signaling & Behavior 7:151-156.10.4161/psb.1893622415043PMC3405696
  19. Sassen, M.M.A. and A.M.C. Wolters-Arts. 1992. Cell-wall texture in shoot apex cells. Acta. Bot. Neerl. 41:25-29.10.1111/j.1438-8677.1992.tb01307.x
  20. Schwuchow, J. and F.D. Sack. 1994. Microtubules restrict plastid sedimentation in protonemata of the moss Ceratodon. Cell Motil. Cytoskel. 29:366-374.10.1002/cm.9702904097859298
  21. Seagull, R.W. 1986. Changes in microtubule organization and wall microfibril orientation during in vitro cotton fiber development: an immunofluorescent study. Can. J. Bot. 64:1373-1381.10.1139/b86-188
  22. Seagull, R.W. 1990. The effect of microtubule and microfilament disrupting agents on cytoskeletal arrays and wall deposition in developing cotton fibers. Protoplasma 159:44-59.10.1007/BF01326634
  23. Szymanski, D.B. and D.J. Cosgrove. 2009. Dynamic coordination of cytoskeletal and cell wall systems during plant cell morphogenesis. Curr. Biol. 19:R800-R811.10.1016/j.cub.2009.07.05619906582
  24. Woeste, K.E., C. Ye and J.J. Kieber. 1999. Two Arabidopsis mutants that overproduce ethylene are affected in the posttranscriptional regulation of 1-aminocyclopropane-1-carboxylic acid synthase. Plant Physiol. 119:521-530.10.1104/pp.119.2.5219952448PMC32129
  25. Yatsu, L.Y. and T.J. Jacks. 1981. An ultrastructural study of the relationship between microtubules and microfibrils in cotton (Gossypium hirsutum L.) cell wall reversals. Am. J. Bot. 68:771-777.10.1002/j.1537-2197.1981.tb12409.x
  26. Yu, Y.B. and S.F. Yang. 1979. Auxin-induced ethylene production and its inhibition by aminoethoxyvinylglycine and cobalt ion. Plant Physiol. 64:1074-1077.10.1104/pp.64.6.107416661095PMC543194
  27. Yuan, M., P.J. Shaw, R.M. Warn and C.W. Lloyd. 1994. Dynamic reorientation of cortical microtubules, from transverse to longitudinal, in living plant cells. PNASU. 91:6050-6053.10.1073/pnas.91.13.6050
  28. Zarembinski, T.I. and A. Theologis. 1994. Ethylene biosynthesis and action: a case of conservation. Plant Mol. Biol. 26:1579-1597.10.1007/BF000164917858205
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :597-603
  • Received Date :2018. 09. 04
  • Accepted Date : 2018. 11. 28