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2021 Vol.34, Issue 5 Preview Page

Research Article

Characterization of Phenotypic Traits and Application of Fruit Flesh Color Marker in Melon (Cucumis melo L.) Accessions

멜론 유전자원의 생육 평가와 과육색 유전형 분석

Ik Hyun Bae1†
,
Han Sol Kang1†
,
Woo Jin Jeong1
,
Jae Hwang Ryu1
,
Oh Hum Lee2
,
Hee Chung3*
배 익현1†
,
강 한솔1†
,
정 우진1
,
유 재황1
,
이 오흠2
,
정 희3*
1Master’s Degree Researcher, Dongoh Seed Corporation R&D Center, Icheon 17405, Korea
2Bachelor’s Degree Researcher, Dongoh Seed Corporation R&D Center, Icheon 17405, Korea
3Post-doc, Dongoh Seed Corporation R&D Center, Icheon 17405, Korea
1농업회사법인 동오시드 주식회사, 석사연구원
2농업회사법인 동오시드 주식회사, 학사연구원
3농업회사법인 동오시드 주식회사, 박사연구원
*Corresponding Author
† These authors contributed equally to this work.
1 October 2021. pp. 478-490
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Abstract

We aimed to generate basic breeding data for melon (Cucumis melo L.). A total of 219 melon accessions conserved at the National Agrobiodiversity Center (NAC) in Rural Development Administration (RDA) were used in this study, of which 72 (33%) were collected from India. The majority of accessions showed orange (42%) and white (36%) flesh color. In addition to phenotypic evaluations, the accessions were genotyped using a molecular marker for the carotenoid biosynthesis gene CmOr. DNA fragments of the expected size were amplified in 205 out of 219 accessions. Digestion of the PCR products with HinfI restriction endonuclease showed 100% concordance between phenotype and genotype in green-fleshed accessions, but 98%, 97%, and 80% concordance in orange-, white-, and creamy-fleshed accessions, respectively. Sequence analysis revealed single nucleotide changes in the three positions of SNP1, SNP2 and SNP1int in the CmOr gene among accessions. These newly found alleles suggest that there are multiple mechanisms in determining fruit flesh color in melon. Also, the phenotype data of diverse accessions obtained in this study will be a valuable source for melon breeding.

Keywords
Agronomic traits
Carotenoid
Genetic resources
Melon
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :5
  • Pages :478-490
  • Received Date : 2021-06-15
  • Revised Date : 2021-09-29
  • Accepted Date : 2021-09-29
  • DOI :https://doi.org/10.7732/kjpr.2021.34.5.478
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