All Issue

2018 Vol.31, Issue 6 Preview Page
December 2018. pp. 695-703

Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a new environment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenic fungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitory effects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showed prominent inhibitory effect of which IC50 was 139.7 ㎍/㎖. Mycelial radii of M. oryzae were measured on PDA medium containing the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fraction showed the impressive inhibitory effect of IC50, 54.12 ㎍/㎖. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almost equivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, the analysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for the verification of cellular mechanism by which the mycelial growth of M. oryzae inhibited.

  1. Chen, S., L. Wang, Z. Que and R. Pan. 2005. Genetic and physical mapping of Pi37(t), a new gene conferring resistance to rice blast in the famous cultivar St. No. 1. Theor. Appl. Genet. 111(8):1563-1570.10.1007/s00122-005-0086-0
  2. Couch, B.C., I. Fudal, M.H. Lebrun, D. Tharreau, B. Valent, P. van Kim, J.L. Notteghem and L.M. Kohn. 2005. Origins of host-specific populations of the blast pathogen Magnaporthe oryzae in crop domestication with subsequent expansion of pandemic clones on rice and weeds of rice. Genetics 170(2):613-630.10.1534/genetics.105.04178015802503PMC1450392
  3. Cho, J.S., L.W. Chi, B.K. Jang, H.S. Jeong and C.H. Lee. 2018. Anti-microbial activities of ten Lauraceae species against Propionibacterium acnes. Korean J. Plant Res. 31(5):423-432.
  4. Davidson, A.L., E. Dassa, C. Orelle and J. Chen. 2008. Structure, function, and evolution of bacterial ATP-binding cassette systems. Microbiol. Mol. Biol. Rev. 72(2):317-364.10.1128/MMBR.00031-0718535149PMC2415747
  5. Dean, M., A. Rzhetsky and R. Allikmets. 2001. The human ATP-binding cassette (ABC) transporter superfamily. Genome Res. 11(7):1156-1166.10.1101/gr.GR-1649R11435397
  6. Dean, R.A., N.J. Talbot, D.J. Ebbole, M.L. Farman, T.K. Mitchell, M.J. Orbach, M. Thon, R. Kulkarni, J.R. Xu, H. Pan, N.D. Read, Y.H. Lee, I. Carbone, D. Brown, Y.Y. Oh, N. Donofrio, J.S. Jeong, D.M. Soanes, S. Djonovic, E. Kolomiets, C. Rehmeyer, W.Li,M. Harding, S. Kim, M.H. Lebrun, H. Bohnert, S. Coughlan, J. Butler, S. Calvo, L.J. Ma, R. Nicol, S. Purcell, C. Nusbaum, J.E. Galagan and B.W. Birren. 2005. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434(7036):980-986.10.1038/nature0344915846337
  7. Di Pietro, A., G. Dayan, G. Conseil, E. Steinfels, T. Krell, D. Trompier, H. Baubichon-Cortay and J. Jault. 1999. P-glycoprotein-mediated resistance to chemotherapy in cancer cells: using recombinant cytosolic domains to establish structure-function relationships. Braz. J. Med. Biol. Res. 32(8):925-939.10.1590/S0100-879X1999000800001
  8. Dixon, R.A., M.J. Harrison and C.J. Lamb. 1994. Early events in the activation of plant defense responses. Annu. Rev. Phytopathol. 32(1):479-501.10.1146/
  9. Dixon, R.A. and C.J. Lamb. 1990. Molecular communication in interactions between plants and microbial pathogens. Annu. Rev. Phytopathol. 41(1):339-367.
  10. Froyd, J.D., C.J. Paget, L.R. Guse, B.A. Dreikorn and J.L. Pafford. 1976. Tricyclazole: a new systemic fungicide for control of Pyricularia oryzae on rice. Phytopathology 66(1):135-131.10.1094/Phyto-66-1135
  11. Gulshan, K. and W.S. Moye-Rowley. 2007. Multidrug resistance in fungi. Eukaryot. Cell 6(11):1933-1942.10.1128/EC.00254-0717873085PMC2168405
  12. Iida, Y., K.B. Oh, M. Saito, H. Matsuoka, H. Kurata, M. Natsume and H. Abe. 1999. Detection of antifungal activity in Anemarrhena asphodeloides by sensitive BCT method and isolation of its active compound. J. Agric. Food Chem. 47(2):584-587.10.1021/jf980707t
  13. Jones, P.M. and A.M. George. 2004. The ABC transporter structure and mechanism: perspectives on recent research. Cell Mol. Life Sci. 61(6):682-699.10.1007/s00018-003-3336-915052411
  14. Jang B.K., L.W. Chi, J.S. Cho and C.H. Lee. 2018. Antimicrobial activity screening of sixty-four evergreen woody species according to extraction conditions against Trichophyton mentagrophytes. Korean J. Plant Res. 31(4):330-341.
  15. Koh, Y.J., B.K. Hwang and H.S. Chung. 1986. Screening of rice cultivars for adult-plant resistance to Pyricularia oryzae. Korean J. Plant Pathol. 2(2):69-81.
  16. Lee, Y.J. 2016. Lysimachia foenum-graecum Herba extract, a novel biopesticide, inhibits ABC transporter genes and mycelial growth of Magnaporthe oryzae. Korean J. Plant Pathol. 32(1):8-15.10.5423/PPJ.OA.08.2015.015726889110PMC4755670
  17. Lee, Y.J., K. Yamamoto, H. Hamamoto, R. Nakaune and T. Hibi. 2005. A novel ABC transporter gene ABC2 involved in multidrug susceptibility but not pathogenicity in rice blast fungus, Magnaporthe grisea. Pestic. Biochem. Phys. 81(1):13-23.10.1016/j.pestbp.2004.07.007
  18. Li, G. and Y.Q. Wang. 2006. Progress in the research on the ABCA gene family of vertebrates. Yi Chuan. 28(8):1015-1022.16870591
  19. Magaldi, S., S. Mata-Essayag, C. Hartung de Capriles, C. Perez, M.T. Colella, C. Olaizola and Y. Ontiveros. 2004. Well diffusion for antifungal susceptibility testing. Int. J. Infect. Dis. 8(1):39-45.10.1016/j.ijid.2003.03.00214690779
  20. Park, H.J., J.Y. Lee, S.S. Moon and B.K. Hwang. 2003. Isolation and anti-oomycete activity of nyasol from Anemarrhena asphodeloides rhizomes. Phytochemistry 64(5):997-1001.10.1016/S0031-9422(03)00462-X
  21. Ribot, C., J. Hirsch, S. Balzergue, D. Tharreau, J.L. Notteghem, M.H. Lebrun and J.B. Morel. 2008. Susceptibility of rice to the blast fungus, Magnaporthe grisea. Plant Physiol. 165(1):114-124.10.1016/j.jplph.2007.06.01317905473
  22. Skamnioti, P. and S.J. Gurr. 2009. Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol. 27(3):141-150.10.1016/j.tibtech.2008.12.00219187990
  23. Valgas, C., S.M.d. Souza, E.F. Smânia and A. Smânia Jr. 2007. Screening methods to determine antibacterial activity of natural products. Braz. J. Microbiol. 38(2):369-380.10.1590/S1517-83822007000200034
  24. Zolnerciks, J.K., E.J. Andress, M. Nicolaou and K.J. Linton. 2011. Structure of ABC transporters. Essays Biochem. 50(1):43-61.10.1042/bse050004321967051
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :695-703
  • Received Date :2018. 11. 26
  • Accepted Date : 2018. 12. 19