• Research Article

    Effect of Gibberellin Treatment on Growth and Flowering Characteristics in the Cultivation of Aquilegia japonica Nakai & H. Hara
    Hoon Geun Oh, Joung Won Lee, Gyeong Ja Lee, Jae Seong Park
    This study was conducted to develop gibberellin treatment technique to enhance flower initiation in Aquilegia japonica Nakai & H. Hara. Seedlings were ... + READ MORE
    This study was conducted to develop gibberellin treatment technique to enhance flower initiation in Aquilegia japonica Nakai & H. Hara. Seedlings were planted in 12㎝-diameter pots on October 2016 and grown in green house. Ambient temperature in the green house was set at minimum 15℃ during day and night to suppress flower initiation at cold temperature condition. Two different types of gibberellin, GA3 and GA4+7, at 4 different concentration levels 100, 200, 400 and 600 ㎎/L, were tested in this study. Gibberellin was sprayed first at planting and secondly at 1-week after planting. Ten to fifteen ㎖ of gibberellin was sprayed for each pot. Plant height and petiole length were elongated by both gibberellin types, flowering was more enhanced by GA3 (91.7∼100%) compared to of GA4+7. However, abnormal flower was less observed in GA3 treatment (0∼16.7%) than GA4+7. Number of flower stalks per plant ranged from 1.9 to 2.5. Number of flowers per plant ranged from 6.8 to 10.3. Differences in flowering characteristics between treatments were statistically significant. Optimal gibberellin treatment to enhance flower initiation in A. japonica Nakai & H. Hara substituting cold treatment was GA3 at the concentration between 400 ㎎/L to 600 ㎎/L. - COLLAPSE
    December 2018
  • Research Article

    Effect of Colchicine on the Growth and Gravitropic Response via Ethylene Production in Arabidopsis Roots
    Seon Woong Kim, Arom Park, Dong Gyu Ahn, Soon Young Kim
    Inhibitory effect of colchicine on growth and gravitropic responses in Arabidopsis root was explored to find whether there was an involvement of ... + READ MORE
    Inhibitory effect of colchicine on growth and gravitropic responses in Arabidopsis root was explored to find whether there was an involvement of ethylene production. It has been known that cytoskeleton components are implicated in sedimentation of statoliths to respond to gravitropism and growth. The root growth was inhibited by 25% and 40% over control for 8 hr treatment of colchicine at a concentration of 10-5 M and 10-7 M, respectively. The roots treated with colchicine at the concentration of 10-7 M showed the same pattern as control in 3 hr, however, gravitropic response was decreased in the next 5 hr. The colchicine treatment at the concentration of 10-5 M inhibited the gravitropic response resulting in 60° of curvature. In order to better understand the role of colchicine, the production of ethylene was measured with and without the treatment of colchicine. Colchicine increased the ethylene production by 20% when compared to control via the activation of ACC oxidase and ACC synthase activity. These results suggest that the inhibition of the growth and gravitropic responses of Arabidopsis roots by the treatment of colchicine could be attributed to the rearrangement of microtubule, and increase of ethylene production. - COLLAPSE
    December 2018
  • Research Article

    Immune Cells Activity, Cytotoxicity and Nitrite Scavenging Activity of Extracts from Several Resource Plants
    Hee-Ock Boo, Jeong-Hun Park, Hyeon-Yong Park
    This study was conducted to explore the immune activity, anticancer activity and nitrile scavenging activities of methanol extracts from the various organs ... + READ MORE
    This study was conducted to explore the immune activity, anticancer activity and nitrile scavenging activities of methanol extracts from the various organs of four Korean resource plants. The immune responses from both human T and B cell line was significantly enhanced in the cell growth compared to control while the cell growth was influenced at a certain period of culture. The results revealed that the cell growth of both human T and B cell was altered in a time dependent manner. Among tested several resource plants, the flower extract of E. japonicum demonstrated a pronounced cytotoxicity against HCT-116 cell with an IC50 value 132.08 ㎍ ㎖-1. The flower extract from Corylopsis coreana had a promising scavenging activity against pH 1.2 compared to other species. Taken together, the studied resource plants have influenced significantly in response to immunity and also have the potential cytotoxicity and nitric scavenging activities. However, the species E. japonicum exhibited the pronounced activities from several resource plants. The result from this investigation suggests that the extracts of studied resource plant could be an addition to basic medicine for some diseases. - COLLAPSE
    December 2018
  • Research Article

    Anti-Inflammatory Activity of Acacia Honey through Inhibition of NF-κB and MAPK/ATF2 Signaling Pathway in LPS-Stimulated RAW264.7 Cells
    Ha Na Kim†, Kun Ho Son†, Hyung Jin Jeong†, Su Bin Park, Jeong Dong Kim, Jin Boo Jeong
    Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been ... + READ MORE
    Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of IL-1β, IL-6 and TNF-α as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated IκB-α degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of NF-κB activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, TNF-α, IL-6, IL-1β and MCP-1 via interruption of the NF-κB and MAPK/ATF2 signaling pathways. - COLLAPSE
    December 2018
  • Research Article

    Changes in Free Amino Acid, Carotenoid, and Proline Content in Chinese Cabbage (Brassica rapa subsp. Pekinensis) in Response to Drought Stress
    Rayhan Ahmed Shawon, Baek Song Kang, Ho Cheol Kim, Sang Gyu Lee, Sung Kyeom Kim, Hee Ju Lee, Jong Hyang Bae, Yang Gyu Ku
    Chinese cabbage grown during autumn season is confronted with drought conditions for a certain period, especially during the early growth stage. In ... + READ MORE
    Chinese cabbage grown during autumn season is confronted with drought conditions for a certain period, especially during the early growth stage. In this study, we investigated the effects of drought stress on plant growth characteristics, as well as free amino acid, carotenoid, and proline in Chinese cabbage. Chinese cabbage seeds (Bulam Plus) were germinated, and all the seedlings were transplanted into plastic containers (28 ㎝ diameter × 22 ㎝ high) containing a commercial growth medium. The soil water content was measured and maintained at 10% for the drought-stressed plants and at 30% for the control plants, for three weeks. The results revealed that plant growth parameters were lower in the drought-stressed plants than in the control plants. The total free amino acid content tended to decrease in both drought-stressed and control plants with time. The total free amino acid content was found to be lower in the drought-stressed plants than in the control plants and the proline content was unaffected. Moreover, at three weeks after treatment, carotenoid content in drought stressed plants was significantly higher than that in the untreated plants. We believe that our study makes a significant contribution to the literature because the effects of drought stress on plant growth parameters, free amino acid, carotenoids, and proline accumulation in autumn growing cultivar of Chinese cabbage have not been widely studied in Korea, and our study provides valuable information in this regard, as Chinese cabbage is consumed throughout the year in Korea. - COLLAPSE
    December 2018
  • Research Article

    Anti-inflammatory Effect of Leaves Extracts from Aralia cordata through Inhibition of NF-κB and MAPKs Signaling in LPS-stimulated RAW264.7 Cells
    Eo Hyun Ji, Da Som Kim, Su Jin Sim, Gwang Hun Park, Jeong Ho Song, Jin Boo Jeong, Nahyun Kim
    Aralia cordata (A. cordata), which belongs to Araliaceae, is a perennial herb widely distributed in East Asia. We evaluated the anti-inflammatory effect ... + READ MORE
    Aralia cordata (A. cordata), which belongs to Araliaceae, is a perennial herb widely distributed in East Asia. We evaluated the anti-inflammatory effect of stems (AC-S), roots (AC-R) and leaves (AC-L) extracted with 100% methanol of A. cordata and elucidated the potential signaling pathway in LPS-stimulated RAW264.7 cells. The AC-L showed a strong anti-inflammatory activity through inhibition of NO production. AC-L dose-dependently inhibited NO production by suppressing iNOS, COX-2 and IL-β expression in LPS-stimulated RAW264.7 cells. AC-L inhibited the degradation and phosphorylation of IκB-α, which donated to the inhibition of p65 nuclear accumulation and NF-κB activation. Furthermore, AC-L suppressed the phosphorylation of ERK1/2 and p38. These results suggested that AC-L may utilize anti-inflammatory activity by blocking NF-κB and MAPK signaling pathway and indicated that the AC-L can be used as a natural anti-inflammatory drugs. - COLLAPSE
    December 2018
  • Research Article

    Discrimination of Floral Scents and Metabolites in Cut Flowers of Peony (Paeonia lactiflora Pall.) Cultivars
    Myung Suk Ahn, Pue Hee Park, Young Nam Kwon, Manjulatha Mekapogu, Suk Weon Kim, Eun Yee Jie, Jae Ah Jeong, Jong Taek Park, Oh Keun Kwon
    Floral scents and metabolites from cut flowers of 14 peony cultivars (Paeonia lactiflora Pall.) were analyzed to discriminate different cultivars and to ... + READ MORE
    Floral scents and metabolites from cut flowers of 14 peony cultivars (Paeonia lactiflora Pall.) were analyzed to discriminate different cultivars and to compare the Korean cultivar with the other cut peonies imported to Korea using electronic nose (E-nose) and Fourier transform infrared (FT-IR) spectroscopy combined with multivariate analysis, respectively. Principal component analysis (PCA) and discriminant function analysis (DFA) dendrogram of peony floral scents were not precisely same but there were 3 groups including same cultivars. PCA and partial least squares-discriminant analysis (PLS-DA) dendrograms of peony metabolites showed that different cut peony cultivars were clustered into two major groups including same cultivars. Fragrance pattern of Korean ‘Taebaek’ was classified to same group with ‘Jubilee’ on the PCA and DFA results and its metabolite pattern was clearly discriminated by the PCA and PLS-DA compared to the other cultivars. These results show that the 14 peony cut flowers could be discriminated corresponding to their chemical relationship and the metabolic profile of Korean ‘Taebaek’ has distinctive characteristics. Furthermore, we suggest that these results could be used as the preliminary data for breeding new cut peony cultivars and for improving the availability of Korean cut peony in cosmetic industry. - COLLAPSE
    December 2018
  • Research Article

    Analysis of Scutellaria baicaleinsis Georgi (Scutellariae Radix) by LC-DAD and LC-ESI/MS
    Youngbob Yu, Pil-Son Choi, Sungtae Koo, Suhwan Chang
    In this study, baicalin, as a marker substance of Scutellariae Radix, was quantitatively analyzed by a high performance liquid chromatography-photodiode array detector ... + READ MORE
    In this study, baicalin, as a marker substance of Scutellariae Radix, was quantitatively analyzed by a high performance liquid chromatography-photodiode array detector (HPLC-DAD). We identified wogonoside, baicalein, and wogonin in the Scutellariae Radix by a high performance liquid chromatography-electrospray ionization-mass spectrometer (HPLC-ESI-MS). The baicalin was separated on a Xterra C18 column (5 ㎛, 4.6 x 250 ㎜) using mobile phase consisting of 38% acetonitrile in 0.68% phosphoric acid. The baicalin spectrum in the Scutellariae Radix extracts was coincided by comparing with UV-visible spectrum (200-550 ㎚) of baicalin standard in the library. The amount of baicalin in Scutellariae Radix was 10.46%, which is higher than KFDA’s guideline. The marker substances of Scutellariae Radix showed a strong base peak [M]+ in the positive detection mode following as; baicalin (m/z; 271 [MH+-sugar]+, 447 [M+H]+), wogonoside (m/z; 285 [MH+-sugar]+, 461 [M+H]+), baicalein (m/z; 271 [M+H]+), wogonin (m/z; 285 [M+H]+). These results are consistent with the fragment pattern and molecular weight of standard components from literature. - COLLAPSE
    December 2018
  • Research Article

    An Investigation of Characteristics of Chinese Bellflower (Platycodon grandiflorum A.) Cultivated Soil
    Jang Nam Choi, Wang Hyu Lee
    In order to understand the characteristics of soil according to the cultivation environment of Chinese bellflower (Platycodon grandiflorum A.), soil chemical properties ... + READ MORE
    In order to understand the characteristics of soil according to the cultivation environment of Chinese bellflower (Platycodon grandiflorum A.), soil chemical properties of 12 collected soil samples from 6 cultivated fields in Okcheon, Chungbuk province in August. 2017 were analyzed. The soil pH was distributed within the range of 4.61 to 5.25 at all cultivation years and E.C (Electric Conductivity) and T-N (Total Nitrogen) of the cultivation year were not significant. Available P2O5 was higher than the average for medicinal crops and P. grandiflorum in Korea and C.E.C (Cation Exchange Capacity) was inconsistent for each cultivation year. In particularly, it was validated that the content of exchangeable cations K, Ca, Ma, and Na in this experiment was similar to that of C.E.C according to the cultivation years, because C.E.C had a high correlation with the exchangeable cations. For the available P2O5, as affected by trans-planting, 5Y-NT-H (cultivated 5 years and non-transplanted) had 58 mg/kg, while 5Y-T-H (cultivated 5 years and transplanted) had 246 ㎎/㎏. The soil pH was found to be lower (acidic) in diseased soils than healthy soils. E.C was confirmed to be was higher in diseased soils than healthy soils except for the one cultivated for 2 years. The contents of T-N and available P2O5 were higher in diseased soil except for the one cultivated for 5 years and 11 years. The exchangeable cation K and Na tended to be higher in diseased soils rather than that in healthy soils, and the exchangeable cation Ca and Mg contents were higher in healthy soils than in diseased soils. The C.E.C of the soil was lower than that of healthy soils in all of the years except for the one which was cultivated for 5 years (transplanted). - COLLAPSE
    December 2018
  • Research Article

    Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts
    Tae-Won Jang, Ji-Soo Choi, Hoi-Ki Kim, Eun-Ja Lee, Man-Wook Han, Ki-Beom Lee, Do-Wan Kim, Jae-Ho Park
    In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared ... + READ MORE
    In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at 121℃, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. IC50 values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material. - COLLAPSE
    December 2018
  • Research Article

    A Successful Regeneration from Shoot Tips of Chrysanthemum morifolium (Ramat.) following Cryopreservation by Droplet-vitrification
    Jung-Yoon Yi, Kotnala Balaraju, Hyung-Jin Baek, Mun-Seop Yoon, Haeng-Hoon Kim, Young-Yi Lee
    This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of Chrysanthemum morifolium (Ramat.) cvs. ‘Borami’ and ‘Yes ... + READ MORE
    This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of Chrysanthemum morifolium (Ramat.) cvs. ‘Borami’ and ‘Yes morning’. The shoot tips of Chrysanthemum were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7 M). Precultured explants were treated with loading solution (LS, C6) containing glycerol 20% and sucrose 20% for 30 min and exposed to dehydration solution (B5) containing 40% of glycerol and 40% of sucrose for 60 min at 25℃, and then transferred onto droplets containing 2.5 ㎕ PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposing of shoot tips to MS + 0.3M sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at 25℃ in both the cultivars. The viability of cooled samples, followed by culturing on NH4NO3-free MS medium for first 5 days was increased to two-fold (80.7%) regrowth rate over those cultured on normal MS medium or MS medium containing plant growth regulators. This result shows droplet-vitrification would be a promising method for cryobanking chrysanthemum germplasm. - COLLAPSE
    December 2018
  • Research Article

    Cryopreservation of Citrus limon (L.) Burm. F Shoot Tips Using a Droplet-vitrification Method
    Jung-Yoon Yi, Kotnala Balaraju, Hyung-Jin Baek, Mun-Seop Yoon, Haeng-Hoon Kim, Young-Yi Lee
    This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: ‘Frost Eureka limon’ and ‘Cook Eureka limon’, using ... + READ MORE
    This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: ‘Frost Eureka limon’ and ‘Cook Eureka limon’, using a droplet-vitrification method. The shoot tips that were excised from in vitro grown seedlings of the two cultivars were preserved in liquid nitrogen (LN) and successfully regenerated into whole plants. Excised shoot tips were pre-cultured for 1 or 2 days in 0.3 M and 0.5 M sucrose solutions at 25℃ and incubated in a loading solution (LS) composed of 17.5% glycerol + 17.5% sucrose in Murashige and Skoog (MS) medium for 40 min at 25℃. Prior to direct immersion in LN for 1 h, the shoot tips were dehydrated with plant vitrification solution 2 (PVS2) at 0℃ or PVS3 at 25℃. The frozen shoot tips were re-warmed and unloaded with 1.2 M sucrose in ½ MS for 30 min at 25℃. Shoot tips were post-cultured overnight on survival medium and then micrografted onto ‘trifoliate orange’ (Poncirus trifoliate (L.) Raf. seedling rootstocks for recovery and to produce whole plants. The highest regrowth rates were 53.5% and 50.3% for cryopreserved shoot tips of ‘Frost Eureka limon’ and ‘Cook Eureka limon’, respectively, when pre-cultured in 0.3 M and 0.5 M sucrose concentrations in a sequencing manner, with LS and treated with PVS2 for 60 min at 0℃. We also investigated whether the ammonium ion concentration on post-culture medium affected the viability of the cryopreserved Citrus shoot tips. The viability of cooled samples, following culturing on woody plant media (WPM) containing ¼ ammonium nitrate overnight before micrografting, was the highest (70.3%) in ‘Frost Eureka limon’. The study described here is a cost-effective and safe method to conserve Citrus fruit cultivars, for the improvement and large-scale multiplication of fruit plants and for breeding disease resistance. - COLLAPSE
    December 2018
  • Research Article

    Anemarrhena asphodeloides Extract Inhibits the Mycelial Growth of Magnaporthe oryzae and Controls the Rice Blast Disease
    Myoung Ho Joo, Yu Mi Yeo, Pil Son Choi, Jae Hyeok Lee, Kwang-Yeol Yang, Young Jin Lee
    Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a new environment friendly biopesticide that has the ... + READ MORE
    Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a new environment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenic fungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitory effects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showed prominent inhibitory effect of which IC50 was 139.7 ㎍/㎖. Mycelial radii of M. oryzae were measured on PDA medium containing the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fraction showed the impressive inhibitory effect of IC50, 54.12 ㎍/㎖. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almost equivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, the analysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for the verification of cellular mechanism by which the mycelial growth of M. oryzae inhibited. - COLLAPSE
    December 2018
  • Research Article

    Changes in Bioactive Compounds and Antioxidant Capacity of Coffee under Different Roasting Conditions
    Jae Lim Song, Theophilus Siaw Asare, Mi Young Kang, Sang Chul Lee
    Roasting has revealed coffee’s potentials as a good source of bioactive compounds. This study was done to investigate the quantitative presence and ... + READ MORE
    Roasting has revealed coffee’s potentials as a good source of bioactive compounds. This study was done to investigate the quantitative presence and activity of bioactive compounds including caffeine, chlorogenic acid (CGA), amino acids, and antioxidant capacity on Coffea arabica L. (Guatemala finca San Sebastian) and C. robusta L. (India Azad Hind). Analysis was performed on Green Bean (GB) Medium-Light (ML), Medium (ME) and Medium-Dark (MD) samples of both varieties. From the results, caffeine content was highest in ME samples of both varieties. GB samples of both varieties had high CGA content which decreased after increasing roasting time and temperature. Most amino acids in GB samples was highest, however, glutamic acid, valine, tyrosine, isoleucine, leucine and phenylalanine had highest quantitative increase in ME samples for both varieties. IC50 of DPPH and ABTS radical scavenging activity was highest in ML samples of both varieties. IC50 of reducing power and total phenolic content was highest in GB sample of both varieties but decreased after increasing roasting conditions. Generally Robusta had the highest quantity of bioactive compounds and antioxidant activity. From this study, the optimal roasting condition for coffee is ME above which there is a significant reduction of bioactive compounds and antioxidant activity. - COLLAPSE
    December 2018
  • Research Article

    Development of ‘Sammany’, a New Variety of Gomchwi with Powdery Mildew Resistance and High Yield
    Jong Taek Suh, Dong Lim Yoo, Ki Deog Kim, Jong Nam Lee, Mi Soon Hong
    A new Gomchwi cultivar ‘Sammany’ was developed by a cross between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis ... + READ MORE
    A new Gomchwi cultivar ‘Sammany’ was developed by a cross between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). Gomchwi is a common Korean name referring wild edible plant species within Ligularia genus. ‘Sammany’ has purple colored petiole ears and petiole trichome is absent. It has 2nd degree leaf vein density. Plant height, leaf length, leaf width and petiole length were 46.2, 19.1, 19.5 and 32.1 ㎝, respectively. Plant height was higher than ‘Gondalbi’. Bolting occurred in mid. July and it flowered from late August to early September. ‘Gondalbi’ bolted and flowered 26 days earlier than ‘Sammany’, and consequently has earlier flowering time more than 26 day. Leaf number of ‘Sammany’ was 156 per plant but ‘Gondalbi’ had 130. ‘Sammany’ had thicker leaves (0.61 ㎜) compared to ‘Gondalbi’ (0.46 ㎜). As a result, yield was higher in ‘Sammany (1,077 g/plant)’ than ‘Gondalbi (798 g/plant)’ and leaf hardness was lower in ‘Sammany (20.8 ㎏/㎠)’ compared to ‘Gondalbi (23.0 ㎏/㎠)’. In addition, ‘Sammany’ was found to be moderately resistant to powdery mildew. With enhanced agronomic and pathology traits, ‘Sammany’ was newly registered as a new Gomchwi cultivar (variety protection no. 131 on May 2017). - COLLAPSE
    December 2018
  • Research Article

    A Study on Antimicrobial Activity of Lysimachia clethroides Duby Root Extracts against Methicillin-resistant Staphylococcus aureus
    Dae Ki Joung, Dong Yeul Kwon, Dong Won Shin
    Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections in humans that are difficult to treat, and as ... + READ MORE
    Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections in humans that are difficult to treat, and as a result, is a substantial contributor to morbidity and mortality. In the present study, in search of natural products capable of inhibiting this multidrug-resistant bacterium, we investigated the antimicrobial activity of Lysimachia clethroides Duby root. The antibacterial activities of EtOH extract of Lysimachia clethroides Duby root and its n-hexane, EtOAc, n-BuOH and water fractions were evaluated against 15 strains of methicillin-resistant staphylococcus aureus (MRSA) and 1 standard methicillin-susceptible S. aureus (MSSA) strain by using the minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test. Antimicrobial activity of n-hexane fraction of Lysimachia clethroides Duby root was remarkable. Against the 16 strains, the minimum inhibitory concentrations (MICs) were in the range of 31.25–62.5 ㎍/㎖ and FICI values for n-hexane fraction of Lysimachia clethroides Duby root+AM and n-hexane fraction of Lysimachia clethroides Duby root+OX were checkerboard method performed using the MRSA, MSSA and one clinical isolate strains via MICI 0.12-1 and 0.25-0.75, showing the increase of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effective combinations could be new promising agents in the management of MRSA. - COLLAPSE
    December 2018