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2023 Vol.36, Issue 6 Preview Page

Research Article

Regeneration and Acclimatization of Regenerants in Long-term in vitro Culture of Japanese Blood Grass (Imperata cylindrica ‘Rubra’)
Eon-Yak Kim1†
,
In-Jin Kang1†
,
Ye-Jin Lee12
,
Baul Yang12
,
Vipada Kantayos3
,
Chang-Hyu Bae45*
1Graduate School Student, Department of Plant Production Sciences, Graduate School of Sunchon National University, Suncheon 57922, Korea
2Ph.D. Course Researcher, Advanced Radiation Technology Institute, Korea Atomic Energy Institute, Jeongup 56212, Korea
3Professor, Department of Botany, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakorn Prathom 73140, Thailand
4Professor, Department of Plant Production Sciences, Graduate School of Sunchon National University, Suncheon 57922, Korea
5Professor, Department of Agricultural Life Sciences, Sunchon National University, Suncheon 57922, Korea
*Corresponding Author
†These authors contributed equally to this work.
1 December 2023. pp. 588-596
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Abstract

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica ‘Rubra’, Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

Keywords
Flow cytometry
In vitro organogenesis
Long-term culture
Plant growth regulators (PGRs)
Sucrose concentration
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :6
  • Pages :588-596
  • Received Date : 2023-11-01
  • Revised Date : 2023-11-22
  • Accepted Date : 2023-11-23
  • DOI :https://doi.org/10.7732/kjpr.2023.36.6.588
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