• Induction of Autophagy by Rosa acicularis Leaves Extracts in RAW264.7 Cells

    인가목(Rosa acicularis Lindl.) 잎 추출물의 대식세포에서 자가포식 유도활성

    Jeong Won Choi, Jin Boo Jeong

    최정원, 정진부

    Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a … + READ MORE
    Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, water extracts from Rosa acicularis leaves (RAL) increased the production of immunostimulatory mediators and phagocytic activity in RAW264.7 cells. RAL increased p62/SQSTM1 expression. Inhibition of TLR4, JNK, and PI3K/AKT blocked RAL-mediated increase of p62/SQSTM1. RAL activated JNK and PI3K/AKT signaling. RAL-mediated activations of JNK and PI3K/AKT signaling were reversed by TLR4 inhibition. Taken together, it is believed that RAL-mediated autophagy may be dependent on activating via TLR4-dependent activation of JNK and PI3K/AKT signaling in macrophages. - COLLAPSE
    1 August 2023
  • Comparative Evaluation of Biological Activities and Active Compounds of Some Invasive Alien Plants

    주요 생태계 교란 외래식물의 생리활성 비교 평가 및 유효성분

    So Jin Kim, Min Gun Kim, Kyung-Hwan Boo, Chang Sook Kim

    김소진, 김민건, 부경환, 김창숙

    To evaluate functional biomaterials of 5 invasive alien plants, total polyphenol and flavonoid contents, antioxidant activity, anti-inflammatory activity, and antibacterial effect were … + READ MORE
    To evaluate functional biomaterials of 5 invasive alien plants, total polyphenol and flavonoid contents, antioxidant activity, anti-inflammatory activity, and antibacterial effect were measured. The total polyphenol and flavonoid contents of the extracts were in the order of Rumex acetosella L. > Hypochaeris radicata L. ≥ Lactuca scariola L. > Humulus japonicus Siebold & Zucc. ≥ Solanum viarum Dunal. The DPPH and ABTS radical scavenging activities of the extract were the highest in R. acetosella and correlated well with the total polyphenol contents. In RAW 264.7 cells stimulated with lipopolysaccharide (LPS), nitric oxide (NO) and prostaglandin E2 (PGE2) production inhibitory effect of the extracts (100 ㎍ SE/mL) were 20~60% and 10~70%, respectively, showing the highest inhibitory effect in R. acetocella. The extracts of R. acetosella, H. japonicus and S. viarum showed antibacterial activity against food poisoning-causing microorganisms such as Bacillus subtilis, Escherichia coli, Vibrio parahaemolyticus and Vibrio vulnificus. Furthermore, the H. japonicus extract was found to have effective antibacterial activity against oral microorganisms such as Enterococcus faecalis, Lacticaseibacillus casei, Rothia dentocariosa, Staphylococcus epidermidis and Streptococcus mutans, and its major active ingredients were predicted to be pentadecylic acid, palmitic acid and clionasterol. These results suggest that alien plants have potential as biomaterials with antioxidant, anti-inflammation and antibacterial effects. - COLLAPSE
    1 August 2023
  • Induction of Autophagy by Paeonia lactiflora Root Extracts through Upregulation p62/SQSTM1 in RAW264.7 Cells

    작약(Paeonia lactiflora) 뿌리 추출물의 대식세포에서 p62/SQSTM1 증가를 통한 자가포식 유도

    Jin Boo Jeong

    정진부

    Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a … + READ MORE
    Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, PLR activated autophagy and increased p62/SQSTM1. The knockdown of p62/SQSTM1 attenuated PLR-mediated autophagy. Inhibition of TLR4 blocked PLR-mediated increase in p62/SQSTM1 level and autophagy induction. In addition, inhibition of PI3K blocked HSL-mediated increase of p62/SQSTM1. PLR increased Nrf2 level and the inhibition of TLR4 and PI3K reduced PLR-mediated increase of Nrf2. Taken together, it is believed that PLR may induce autophagy through upregulating p62/SQSTM1 via TLR4/PI3K/Nrf2 signaling pathway. - COLLAPSE
    1 August 2023
  • Protective Effect of Glycyrrhiza New Domestic Variety on the Acute Pancreatitis

    국내 감초 신품종의 급성 췌장염 보호 효과

    Dong-Gu Kim, Kyung Hyun Min

    김동구, 민경현

    Glycyrrhiza korshinskyi Grig. (Gk), which is a new variety of Glycyrrhiza, was developed by the National Institute of Horticultural and Herbal … + READ MORE
    Glycyrrhiza korshinskyi Grig. (Gk), which is a new variety of Glycyrrhiza, was developed by the National Institute of Horticultural and Herbal Science in Korea Rural Development Administration. It is improved the limitations of traditional Glycrrhiza species such as G. uralensis Fisch., G. glabra L., and G. inflata Batal. and has been reported various pharmacological effects. The aim of this study is to investigate the protective effect of Gk on the acute pancreatitis, because which has not been revealed yet. AP was induced via intraperitoneal injection of cerulein (50 ㎍/㎏) hourly for 7 times in C57BL/6 mice. Gk water extract (20 or 50 ㎎/㎏) or saline was administrated via oral gavage 1 h before the first injection of cerulein. The mice were sacrificed at 5 h after the final injection of cerulein. The inflammatory cell infiltration in pancreatic tissue was decreased by the administration of Gk water extract compared to the AP group. Also, serum amylase and lipase levels were decreased by the administration of Gk water extract compared to the AP group. These results suggest that Gk have protective effect on the acute pancreatitis. - COLLAPSE
    1 August 2023
  • Development of Chloroplast Genome-based Insertion/Deletion Markers in the Genus Broussonetia

    닥나무 속 식물의 엽록체 유전체 기반 InDel 마커의 개발

    Eun Jee Lee, Yoon A Kim, Mi Sun Lee, Ju Hyeok Kim, Young Kyu Choi, Jung Sung Kim, Chang Seob Sin, Yi Lee

    이은지, 김윤아, 이미선, 김주혁, 최용규, 김정성, 신창섭, 이이

    Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of … + READ MORE
    Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia. - COLLAPSE
    1 August 2023
  • New Record of Alien Plants, Stellaria pallida, S. ruderalis, and Cerastium pumilum (Caryophyllaceae)

    미기록 외래식물 모래별꽃(Stellaria palida), 들별꽃(S. ruderalis), 애기점나도나물(Cerastium pumilum)의 보고

    Eun Su Kang, Jin Suk Kim, Seon Min Kim, Kang-Hyup Lee, Dong Chan Son

    강은수, 김진숙, 김선민, 이강협, 손동찬

    Stellaria pallida (Dumort.) Crép., S. ruderalis M. Lepší, P. Lepší, Z. Kaplan & P. Koutecký, and Cerastium pumilum Curtis are unrecorded alien … + READ MORE
    Stellaria pallida (Dumort.) Crép., S. ruderalis M. Lepší, P. Lepší, Z. Kaplan & P. Koutecký, and Cerastium pumilum Curtis are unrecorded alien species of Caryophyllaceae found in Seoul and Gyeonggi-do in the Republic of Korea. Stellaria pallida is readily distinguished from other taxa in the same genus by its petals, as it’s considerably smaller than the sepals or absent. In contrast, S. ruderalis is difficult to identify as it has intermediate traits between S. media L. and S. neglecta (Lej.) Weihe. However, S. ruderlais clearly identified by seed morphology, and S. ruderalis, unlike S. media and S. neglecta, has conical shape of outer periclinal wall and papillate on the basely surface of that. Cerastium pumilum is similar to C. glomeratum Thuill. and belongs to the same subgenus (Sub gen. Fugacia); however, it has some distinct characteristics, including stamens that are primarily 8 (5-10), upper bracts with membranous edges, and the length of the pedicels and petals being longer than that of the sepals. The three of alien plants was recorded for the first time in this study, and information of their habitat, distribution map, description and photographs are presented. - COLLAPSE
    1 August 2023
  • Floristic Study of Vascular Plants in Yeonhwasan Mt. (Taebaek-si, Gangwon-do, Korea)

    연화산(강원도 태백시)의 관속식물상

    Hyeon Jin Jeong, Ju Eun Jang, Aleksey L. Kim, Se Ryeong Lee, Young-Soo Kim, Ji Eun Kim, Jung Sim Lee, Kang Hyup Lee, Eun Su Kang, Sang-Kuk Han, Hee-Young Gil

    정현진, 장주은, 김알렉세이, 이세령, 김영수, 김지은, 이정심, 이강협, 강은수, 한상국, 길희영

    Vascular plants of Yeonhwasan Mt., located in Taebaek-si, Gangwon-do, were investigated. Field surveys were conducted fourteen times between April and September of … + READ MORE
    Vascular plants of Yeonhwasan Mt., located in Taebaek-si, Gangwon-do, were investigated. Field surveys were conducted fourteen times between April and September of 2022. A total of 505 taxa were found consisting of 87 families, 282 genera, 441 species, 15 subspecies, 41 varieties, and 8 forma. Among them, the endemic plants of Korea were 22 taxa. The number of threatened and near threatened Red List plants designated by the Korean National Arboretum was four, comprising each one of critically endangered (CN), endangered (EN), vulnerable (VU), and near threatened (NT) species. The number of floristic target plants designated by the Ministry of Environment was 102, including one taxon of level V, 9 taxa of level IV, and 34 taxa of level III. Invasive alien plants were 34 taxa, and three taxa were ecosystem disturbance plants. The urbanization index of investigated site was 12.8%, and the naturalization index was 6.7%. To wisely manage Yeonhwasan, measures for preserving biodiversity and preventing invasive plants are needed. - COLLAPSE
    1 August 2023
  • Distribution of Plant Resources in Mt. Baekseok (Pyeongchang-gun, Gangwon-do)

    백석산(강원도 평창군) 식물자원의 분포

    Jun-Hee Jeong, Ki-Oug Yoo

    정준희, 유기억

    Distribution of plant resources in Mt. Baekseok located at Pyeongchang-gun, Gangwon-do, were surveyed for a total 17 times from April 2021 to … + READ MORE
    Distribution of plant resources in Mt. Baekseok located at Pyeongchang-gun, Gangwon-do, were surveyed for a total 17 times from April 2021 to September 2022. The result of this survey revealed 628 taxa, consisting of 99 families, 346 genera, 552 species, 20 subspecies, 49 varieties, 6 forms, and one hybrid. Among them, 21 taxa were endemic plants to Korea, 12 taxa were red list plants by the Ministry of Environment and 560 taxa were red list plants by the Korea Forest Service. The floristic target species amounted to 164 taxa, specifically one taxon of grade Ⅴ, 20 taxa of grade IV, 52 taxa of grade III, 53 taxa of grade II, and 38 taxa of grade I. In addition, 34 taxa were classified as plants adaptable to climate change. 42 taxa of alien plants and 3 taxa of ecosystem disturbance species were also found in this area. Useful plants listed consists of 246 taxa (39.2%) of edible plants, 215 taxa (34.2%) of pasture plants, 187 taxa (29.8%) of medicinal plants, 75 taxa (11.9%) of ornamental plants and 22 taxa (3.5%) of timber plants, respectively. - COLLAPSE
    1 August 2023
  • An Analysis of the Heritability of Phenotypic Traits Using Chloroplast Genomic Information of Legume Germplasms

    엽록체 유전정보를 이용한 두류 유전자원 형태적 형질의 유전력 분석

    Dong Su Yu, Yu-Mi Choi, Xiaohan Wang, Manjung Kang

    유동수, 최유미, 왕샤오한, 강만정

    Developing and breeding improved legume-based food resources require collecting useful genetic traits with heritability even though requiring some time-consuming, costly, and labor … + READ MORE
    Developing and breeding improved legume-based food resources require collecting useful genetic traits with heritability even though requiring some time-consuming, costly, and labor intensive. We attempted to infer heritability of nine genetic traits—days to flowering, days to maturity, period from flowering to maturity, the number of seeds per pod, 100-seeds weight, and four contents such as crude protein, crude oil, crude fiber, and dietary fiber—using 455 homologous chloroplast gene sets of six species of legumes. Correlation analysis between genetic trait differences and phylogenetic distance of homologous gene sets revealed that days to flowering, the number of seeds per pod, and crude oil content were influenced by genetic factors rather than environmental factors by 62.86%, 69.45%, 57.14% of correlated genes (P-value ≤ 0.05) and days to maturity showed intermediate genetic effects by 62.42% (P-value ≤ 0.1). The period from flowering to maturity and 100-seeds weight showed different results compared to those of some previous studies, which may be attributed to highly complicated internal (epistatic or additive gene effects) and external effects (cultural environment and human behaviors). Despite being slightly unexpected, our results and method can widely contribute to analyze heritability by including genetic information on mitochondria, nuclear genome, and single nucleotide polymorphisms. - COLLAPSE
    1 August 2023
  • Effect of Temperature Conditions and Chemical Treatments on Seed Germination of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz.

    봉래꼬리풀의 종자 발아에 미치는 온도 조건과 화학적 처리의 영향

    Dong-Hak Kim, Young-Eun Kim, Seungju Jo, Jong-Won Lee, Sang-Jun Kim

    김동학, 김영은, 조승주, 이종원, 김상준

    The germination characteristics of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz., a rare and endemic plant designated by the Korea Forest Service, were … + READ MORE
    The germination characteristics of Pseudolysimachion kiusianum var. diamantiacum (Nakai) T.Yamaz., a rare and endemic plant designated by the Korea Forest Service, were investigated according to light conditions, temperature, and pretreatment. As a result of the germination experiment according to light conditions, it was determined that P. kiusianum is a photoblastic seed that does not germinate at all in dark conditions. The optimum germination temperature of the seeds was found to be 20-25℃, considering the final germination rate and germination time. Three growth regulators (IAA, GA3, kinetin) and two inorganic salts (KNO3, KCl) were pretreated to improve the germination rate of P. kiusianum seeds. The growth regulators IAA and kinetin had no significant effect on improving the germination rate of P. kiusianum seeds. On the other hand, GA3 significantly increased the final germination percentage and germination rate regardless of the concentration, especially the treatment of more than 500 ㎎·L-1 at 20℃ was more than 4 times more effective than the untreated. The inorganic salts KNO3 and KCl had no significant effect on the seeds of P. kiusianum at low concentrations, but at higher concentrations (40 mM and 300 mM, respectively), they improved the germination rate and germination age by 2 times compared to the untreated. The results of this study will be useful for the mass propagation of P. kiusianum, which has the potential to be utilized as a native plant for restoration. - COLLAPSE
    1 August 2023
  • Propagation Characteristics of Rare Plants Diabelia spathulata (Siebold & Zucc.) Landrein Applied by Seeding and Cutting Methods

    종자파종과 삽목을 활용한 희귀식물 주걱댕강나무의 증식 특성

    Jeong Gul Jang, Myung Hoon Yi

    장정걸, 이명훈

    The purpose of this study was to identify the propagation characteristics of Diabelia spathulata (Siebold & Zucc.) Landrein through seeding and cutting … + READ MORE
    The purpose of this study was to identify the propagation characteristics of Diabelia spathulata (Siebold & Zucc.) Landrein through seeding and cutting methods. The seed germination rate was meager (0.22~3.56%). In hardwood cutting, the highest rooting rate was 36.1% in sand + perlite (2:1) and 37.8% in 1000 ㏙ of IBA pre-treatment. Softwood cutting showed the highest rooting rate when the cutting diameter was 3 to 5 ㎜ and the cutting length was 7 to 8 ㎝. In particular, the highest rooting rate of 80% was observed in the sand among the 8 cultivation media treatments. In the case of root cuttings, the rooting rate was 83.3~96.7% in all cultivation media. The highest rooting rate was observed when the diameter of the cutting was 7 to 8 ㎜, and the length of the cutting was about 10 to 11 ㎝. Among cutting methods, root cutting showed the highest rooting rate. Division showed a 100% rooting rate regardless of size or growth status. For Diabelia spathulata (Siebold & Zucc.) Landrein, propagation by cuttings was more efficient than seeding. However, Further research on the morphophysiological dormancy and dormancy break of seeds is needed for seed propagation. - COLLAPSE
    1 August 2023
  • Flowering, Fertilization Characteristics and Speedy Breeding in a Protandry Plant Peucedanum japonicum Thunb.

    웅예선숙 식물인 갯기름나물(식방풍)의 개화 특성 및 세대촉진 방법

    Jin Tae Kim, Ho Jun Joh, Hong Seob Yu, Young Sang Park, Ji-Seok Kim, Yong Hyeok Jeong, Tae-Jin Yang

    김진태, 조호준, 유홍섭, 박영상, 김지석, 정용혁, 양태진

    Peucedanum japonicum Thunberg is a biennial plant that usually flowers and dies after seed setting at 2nd year. We inspected the … + READ MORE
    Peucedanum japonicum Thunberg is a biennial plant that usually flowers and dies after seed setting at 2nd year. We inspected the flowering and fertilization characteristics to establish the breeding system. The plant forms complex umbel inflorescences consisting of multiple umbellets and numerous flowers. Each flower is bisexual but allogamous because of protandry. A flower bloomed for three days with matured pollen and no stigma appearance. Inspection of pollen tube growth indicates that the pollen viability can be maintained for up to 3~7 days after first flowering (DAF). In a umbellet, flowers bloomed during 1~5 DAF and the pollen maintained viability during 3~10 DAF, and the pistil developed and maintained fertility during 8~12 DAF. Theoretically, self-pollination can be possible during 8~10 DAF in the same umbellet. Approximately 4.4% of seeds were developed by self-pollination among flowers in an enveloping solitary umbel. There is at least a 9~10 days difference for the development of an umbel with another umbel at the next branch, which indicates pollination can be permitted between different branches in an individual plant. Finally, we have established a rapid breeding method to shorten the breeding cycle to one year from two years by integrating the flowering and pollination characteristics. - COLLAPSE
    1 August 2023
  • Characterization of Agronomic Traits and Evaluation of Lignan Contents in Asian and African Sesame (Sesamum indicum L.) Germplasms

    아시아 및 아프리카 원산 참깨(Sesamum indicum L.) 유전자원의 농업형질과 리그난 함량 평가

    Sookyeong Lee, Jungsook Sung, Gi-An Lee, Eunae Yoo, So Jeong Hwang, Weilan Li, Tae-Jin Yang

    이수경, 성정숙, 이기안, 유은애, 황소정, 리웨이란, 양태진

    Sesame (Sesamum indicum L.) is an ancient oilseed crop, which is usually cultivated for its seeds. Sesame breeding aims to achieve … + READ MORE
    Sesame (Sesamum indicum L.) is an ancient oilseed crop, which is usually cultivated for its seeds. Sesame breeding aims to achieve high seed yield and quality, along with resistance to biotic or abiotic stresses. It is estimated that sesame is originated from Asia or Africa continent. In this study, we characterized 10 agronomic traits and evaluated lignan contents in 165 sesame germplasm originated from Asia or Africa, to select high-yield or high-lignan content accessions. Sesame germplasm showed diverse phenotypes and highly variable lignan contents (sesamin: 0.5-12.6 ㎎/g, sesamolin: 0.1-3.5 ㎎/g, lignan: 1.1-16.1 ㎎/g). Based on originated continent, there are significant difference in agronomic traits, but no in lignan content. Correlation analysis revealed that yield-related agronomic traits were negatively related with lignan contents. Also, PCA analysis showed that most agronomic traits and lignan contents were principal components explaining diversity of whole sesame germplasm. Sesame germplasm was clustered into three groups based on agronomic traits and lignan contents. Finally, we selected high-yield (IT29416, IT167042, K276848, K276849) and high-lignan candidate accessions (IT169254, IT170031, IT169250, IT154876, IT170034), respectively. These accessions are expected to be valuable resources for breeding of high-yield and high-lignan contents functional cultivars. - COLLAPSE
    1 August 2023