Adenophorae Radix Attenuates Mast Cell-mediated Allergic Inflammation through Down-regulation of NF-κB/ Caspase-1 Activation

Noh-Yil Myung

doi:10.7732/kjpr.2020.33.6.659

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2020 Vol.33, Issue 6 Preview Page Next Page

Research Article

Adenophorae Radix Attenuates Mast Cell-mediated Allergic Inflammation through Down-regulation of NF-κB/ Caspase-1 Activation

1 December 2020. pp. 659-665

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Abstract

Adenophorae Radix (AR) has been used as a traditional medicine for various diseases. However, the regulatory mechanisms of AR in allergic inflammation are not yet understood. The present study was conducted to investigate the effect and mechanisms of AR on the mast cell-mediated allergic response. To determine the pharmacological mechanisms of AR in allergic inflammation, we evaluated the effects of AR on the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and IL-8 as well as the activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). Our results demonstrated that AR effectively attenuated the PMACI-induced production of TNF-α, IL-6, IL-1β and IL-8 in stimulated HMC-1. Additionally, we showed that the inhibitory effect of AR on inflammatory cytokines in PMACI-stimulated HMC-1 cells involved the suppression of the activation NF-kB/caspase-1 in PMACI-stimulated HMC-1. Collectively, these findings provide experimental evidence that AR may be a useful candidate for the treatment of allergic inflammation.

Keywords

Adenophorae Radix

Allergic inflammation

Caspase-1

Inflammatory cytokines

Mast cells

Nuclear factor-κB

References

Birrell, M.A., E. Hardaker, S. Wong, K. McCluskie, M. Catley, J. De Alba, R. Newton, S. Haj-Yahia, K.T. Pun, C.J. Watts, R.J. Shaw, T.J. Savage and M.G. Belvisi. 2005. Ikappa-B kinase-2 inhibitor blocks inflammation in human airway smooth muscle and a rat model of asthma. Am. J. Respir. Crit. Care Med. 172:962-971. 10.1164/rccm.200412-1647OC16002568

Bouchier-Hayes, L. and S.J. Martin. 2004. CARDINAL roles in apoptosis and NFkappaB activation. Vitam. Horm. 67:133-147. 10.1016/S0083-6729(04)67008-7

Caughey, G.H. 2016. Mast cell proteases as pharmacological targets. Eur. J. Pharmacol. 778:44-55. 10.1016/j.ejphar.2015.04.04525958181PMC4636979

DiDonato, J.A., F. Mercurio and M. Karin. 2012. NF-κB and the link between inflammation and cancer. Immunol. Rev. 246:379-400. 10.1111/j.1600-065X.2012.01099.x22435567

Edwards, M.R., K. Strong, A. Cameron, R.P. Walton, D.J. Jackson and S.L. Johnston. 2017. Viral infections in allergy and immunology: How allergic inflammation influences viral infections and illness. J. Allergy Clin. Immunol. 140:909-920. 10.1016/j.jaci.2017.07.02528987220PMC7173222

Furue, M. and T. Kadono. 2017. "Inflammatory skin march" in atopic dermatitis and psoriasis. Inflamm. Res. 66:833-842. 10.1007/s00011-017-1065-z28620798

Gilmore, T.D. and M.R. Garbati. 2011. Inhibition of NF-κB signaling as a strategy in disease therapy. Curr. Top. Microbiol. Immunol. 349: 245-263. 10.1007/82_2010_10521113699

Han, N.R., P.D. Moon, N.R. Kim, H.Y. Kim, H.J. Jeong and H.M. Kim. 2017. Schisandra chinensis and its main constituent schizandrin attenuate allergic reactions by down-regulating caspase-1 in ovalbumin-sensitized mice. Am. J. Chin. Med. 45:159-172. 10.1142/S0192415X1750011228081628

Hawiger, J. 2001. Innate immunity and inflammation: A transcriptional paradigm. Immunol. Res. 23:99-109. 10.1385/IR:23:2-3:099

Kim, H.J. and S.H. Kang. 2017. Ethnobotany, phytochemistry, pharmacology of the Korean Campanulaceae: A comprehensive review. Korean J. Plant Res. 30:240-264. 10.7732/kjpr.2017.30.2.240

Kim, H.Y., S.Y. Nam, S.Y. Hwang, H.M. Kim and H.J. Jeong. 2016. Atractylone, an active constituent of KMP6, attenuates allergic inflammation on allergic rhinitis in vitro and in vivo models. Mol. Immunol. 78:121-132. 10.1016/j.molimm.2016.09.00727636508

Kim, J.Y., K.W. Park, K.D. Moon, M.K. Lee, J. Choi, S.T. Yee, K.H. Shim and K.I. Seo. 2008. Induction of apoptosis in HT-29 colon cancer cells by crude saponin from Platycodi Radix. Food Chem. Toxicol. 46:3753-3758. 10.1016/j.fct.2008.09.06718955103

Kim, S.J., M.C. Kim, J.Y. Um and S.H. Hong. 2010. The beneficial effect of vanillic acid on ulcerative colitis. Molecules 15:7208-7217. 10.3390/molecules1510720820959795PMC6259113

Kuida, K., J.A. Lippke and G. Ku. 1995. Altered cytokine export and apoptosis in mice deficient in interleukin‐1B converting enzyme. Science 267:2000-2002. 10.1126/science.75354757535475

Lamkanfi, M., M. Kalai, X. Saelens, W. Declercq and P. Vandenabeele. 2004. Caspase-1 activates nuclear factor of the kappa-enhancer in B cells independently of its enzymatic activity. J. Biol. Chem. 279:24785-24793. 10.1074/jbc.M40098520015039421

Lee, Y.S., S.B. Han, H.J. Ham, J.H. Park, J.S. Lee, D.Y. Hwang, Y.S. Jung, D.Y. Yoon and J.T. Hong. 2020. IL-32γ suppressed atopic dermatitis through inhibition of miR-205 expression via inactivation of nuclear factor-kappa B. J. Allergy Clin. Immunol. 146:156-168. 10.1016/j.jaci.2019.12.90531931018

Li, J.W., Y. Liu, B.H. Li, Y.Y. Wang, H. Wang and C.L. Zhou. 2016. A polysaccharide purified from Radix Adenophorae promotes cell activation and pro-inflammatory cytokine production in murine RAW264.7 macrophages. Chin. J. Nat. Med. 14:370-376.

Lim, J.M., B. Lee, J.H. Min, E.Y. Kim, J.H. Kim, S. Hong, J.J. Kim, Y. Sohn and H.S. Jung. 2018. Effect of peiminine on DNCB-induced atopic dermatitis by inhibiting inflammatory cytokine expression in vivo and in vitro. Int. Immunopharmacol. 56:135-142. 10.1016/j.intimp.2018.01.02529414643

Martin, R.A., J.L. Ather, L.K. Lundblad, B.T. Suratt, J.E. Boyson, R.C. Budd, J.F. Alcorn, R.A. Flavell, S.C. Eisenbarth and M.E. Poynter. 2013. Interleukin-1 receptor and caspase-1 are required for the Th17 response in nitrogen dioxide-promoted allergic airway disease. Am. J. Respir. Cell Mol. Biol. 48:655-664. 10.1165/rcmb.2012-0423OC23371061PMC3707382

Mitchell, J.P. and R.J. Carmody. 2018. NF-κB and the transcriptional control of inflammation. Int. Rev. Cell Mol. Biol. 335:41-84. 10.1016/bs.ircmb.2017.07.00729305014

Modena, B.D., K. Dazy and A.A. White. 2016. Emerging concepts: mast cell involvement in allergic diseases. Transl. Res.174: 98-121. 10.1016/j.trsl.2016.02.01126976119

Sakamoto, T., W. Ohashi, K. Tomita , K. Hattori , N. Matsuda and Y. Hattori. 2018. Anti-inflammatory properties of cilostazol: Its interruption of DNA binding activity of NF-kappaB from the Toll-like receptor signaling pathways. Int Immunopharmacol. 62:120-131. 10.1016/j.intimp.2018.06.02130005227

Siegmund, B., H.A. Lehr, G. Fantuzzi and C.A. Dinarello. 2001. IL-1 betaconverting enzyme (caspase-1) in intestinal inflammation. PNASU. 98:13249-13254. 10.1073/pnas.23147399811606779PMC60856

Song, P., J. Xia, C. Rezeng, L. Tong and W. Tang. 2016. Traditional, complementary, and alternative medicine: Focusing on research into traditional Tibetan medicine in China. Biosci. Trends 10:163-170. 10.5582/bst.2016.0110527301588

Theoharides, T.C., I. Tsilioni and H. Ren. 2019. Recent advances in our understanding of mast cell activation - or should it be mast cell mediator disorders? Expert Rev. Clin. Immunol. 15:639-656. 10.1080/1744666X.2019.159680030884251PMC7003574

Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 33
No :6
Pages :659-665
Received Date : 2020-09-16
Revised Date : 2020-11-20
Accepted Date : 2020-11-25
DOI :https://doi.org/10.7732/kjpr.2020.33.6.659

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

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