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2018 Vol.31, Issue 5 Preview Page

Research Article

Intraplant Variations of Sesquiterpene Lactone Content in Lettuce Genetic Resources Grown in Two Cultivation Seasons
Awraris Derbie Assefa1
,
Susanna Choi2
,
Jung-Sook Sung3
,
On-Sook Hur3
,
Na-Young Ro3
,
Sok Young Lee4
,
Ho-Sun Lee3
,
Jae Eun Lee3
,
Ju-Hee Rhee4*
1Post-doc, National Agrobiodiversity Center, NAS, RDA
2Master’s Degree Researcher, National Agrobiodiversity Center, NAS, RDA
3Researcher, National Agrobiodiversity Center, NAS, RDA
4Senior Scientist, National Agrobiodiversity Center, NAS, RDA
* Corresponding Author
31 October 2018. pp. 538-546
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Abstract

Inflorescence, stem, and leaf samples of lettuce grown in a greenhouse in spring and autumn seasons were assayed for sesquiterpene lactones (SLs) content by high performance liquid chromatography. The concentrations of SLs were significantly higher in the inflorescences followed by upper leaf and stem compared to the other plant parts in most of the samples. SLs content (sum of lactucin and lactucopicrin) in various tissues of lettuce cultivated in spring season varied from 5.7 to 22.5 fold ranging from 27.4 ㎍/g dry weight (DW) in the upper stem (cultivar “PI 176588”) as the lowest to as high as 2,292.0 ㎍/g DW in the inflorescence (cultivar “709849-1”). During autumn cultivation, the concentration of SLs varied from 2.0 to 14.4 fold ranging from as low of 32.4 ㎍/g DW in the lower stem (cultivar “PI176588”) to as high of 838.0 ㎍/g DW in the upper leaf (cultivar “Dambaesangchu”). Higher lactucin (1.2 to 5.6 fold) and lactucopicrin (1.1 to 3.9 fold) concentration was observed during spring compared to autumn cultivation in most of the samples. SLs content in various organs of lettuce increases from the basal plant part going upwards. As lactucin and lactucopicrin are the major SLs which affects the sensory property of lettuce, their quantitative variation in the lettuce cultivars is useful for breeding new varieties with better consumer acceptance.

Keywords
Inflorescence
Lactuca sativa
Lactuca serriola
Lactucin
Lactucopicrin
Leaf
Stem
References
1
Bunning, M.L., P.A. Kendall, M.B. Stone, F.H. Stonaker and C. Stushnoff. 2010. Effects of seasonal variation on sensory properties and total phenolic content of 5 lettuce cultivars. J. Food Sci. 75(3):S156-S161.
10.1111/j.1750-3841.2010.01533.x20492312
2
Cho, G.T., Y.Y. Lee, S. Choi, J.Y. Yi, G.A. Lee, H.C. Ko, Y.W. Na, H.J. Park, K.H. Ma and J.G. Gwag. 2016 Variation of sesquiterpene lactones content in lettuce (Lactuca sativa L. germplasm. J. Korean Soc. Int. Agric. 28:220-226 (in Korean).
10.12719/KSIA.2016.28.2.220
3
Chadwick, M., H. Trewin, F. Gawthrop and C. Wagstaff. 2013. Sesquiterpenoids lactones: Benefits to plants and people. Int. J. Mol. Sci. 14:12780-12805.
10.3390/ijms14061278023783276PMC3709812
4
Ferioli, F., M.A. Manco and L.F.D. Antuono. 2015. Variation of sesquiterpene lactones and phenolics in chicory and endive germplasm. J. Food Compost. Anal. 39:77-86.
10.1016/j.jfca.2014.11.014
5
Foster, J.G., K.A. Cassida and M.A. Sanderson. 2011. Seasonal variation in sesquiterpene lactone concentration and composition of forage chicory (Cichorium intybus L.) cultivars. Grass Forage Sci. 66(3):424-433.
10.1111/j.1365-2494.2011.00801.x
6
Graziani, G., R. Ferracane, P. Sambo, S. Santagata, C. Nicoletto and V. Fogliano. 2015. Profiling chicory sesquiterpene lactones by high resolution mass spectrometry. Food Res. Int. 67:193-198.
10.1016/j.foodres.2014.11.021
7
Jang, M., J.B. Seo, M.S. Jo, K.C. Ma, J.W. Lee, B.K. Yoon and Y.S. Yoon. 2013. Functional Contents in Varieties of indigenious Lettuce (Lactuca sativa L.). Korean J. Plant Res. 10:109-109 (in Korean).
8
Kim, D.E., X. Shang, A.D.Assefa, Y.S. Keum and R.K. Saini. 2018. Metabolite profiling of green, green/red, and red lettuce cultivars: Variation in health beneficial compounds and antioxidant potential. Food Res. Int. 105:361-370.
10.1016/j.foodres.2017.11.02829433225
9
Martínez-Sánchez, A., M.C. Luna, M.V. Selma, J.A. Tudela, J. Abad and M.I. Gil. 2012. Baby-leaf and multi-leaf of green and red lettuces are suitable raw materials for the fresh-cut industry. Postharvest Biol. Tech. 63(1):1-10.
10.1016/j.postharvbio.2011.07.010
10
Mithöfer, A. and W. Boland. 2012. Plant defense against herbivores: Chemical aspects. Annu. Rev. Plant Biol. 63(1):431-450.
10.1146/annurev-arplant-042110-10385422404468
11
Pollard, J., S.F.L. Kirk and J.E. Cade. 2002. Factors affecting food choice in relation to fruit and vegetable intake: a review. Nutr. Res. Rev. 15(2):373-387.
10.1079/NRR200244
12
Price, K.R., M.S. Dupont, R. Shepherd, H.W.S. Chan and G.R. Fenwick. 1990. Relationship between the chemical and sensory properties of exotic salad crops-coloured lettuce (Lactuca sativa) and chicory (Cichorium intybus). J. Sci. of Food Agr. 53(2):185-192.
10.1002/jsfa.2740530206
13
Rees, S.B. and B.J. Harborne. 1985. The role of sesquiterpene lactones and phenolics in the chemical defence of the chicory plant. Phytochemistry 24(10):2225-2231.
10.1016/S0031-9422(00)83015-0
14
Seo, M.W., D.S. Yang, S.J. Kays, G.P. Lee and K.W. Park. 2009. Sesquiterpene lactones and bitterness in korean leaf lettuce cultivars. HortScience 44(2):246-249.
15
Sung, J.S., O.S. Hur, K.Y. Ryu, H.J. Baek, S. Choi, S.G. Kim, P.L. Binod, H.C. Ko, J.G. Gwak and J.H. Rhee. 2016. Variation in phenotypic characteristics and contents of sesquiterpene lactones in lettuce (Lactuca sativa L.) germplasm. Korean J. Plant Res. 10:115-115.
10.7732/kjpr.2016.29.6.679
16
Tamaki, H., R.W. Robinson, J.L. Anderson and G.S. Stoewsand. 1995. Sesquiterpene lactones in virus-resistant lettuce. J. Agr. Food Chem. 43(1):6-8.
10.1021/jf00049a002
17
UPOV, 1981. Guidelines for the conduct of tests for distinctness, homogeneity and stability, lettuce (Lactuca sativa L.). Document No. TG/13/4 (26.10.1981).
18
Zangerl, A. and F. Bazzaz. 1993. Theory and pattern in plant defense allocation: In Fritz, R.S. and E.L. Simms (eds.), Plant Resistance to Herbivores and Pathogens, University of Chicago Press, Chicago, IL (USA). pp. 363-391.
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :5
  • Pages :538-546
  • Received Date : 2018-06-18
  • Revised Date : 2018-08-10
  • Accepted Date : 2018-08-16
  • DOI :https://doi.org/10.7732/kjpr.2018.31.5.538
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