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2020 Vol.33, Issue 2

Research Article

Isolation of Bioactive Compounds from the Ethylacetate Fraction of Fermented Garlic Complex and their Tyrosinase Inhibition Activities

대산(大蒜)을 포함하는 복합발효물의 에틸아세테이트 분획으로부터 Tyrosinase 저해활성 성분의 분리 및 동정

Hyo-Nam Song1*
,
Nam-In Baek2
송 효남1*
,
백 남인2
1Professor, School of Food and Nutrition Science for Bioindustry, Semyung University, Jecheon 27136, Korea
2Professor, Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University, Yongin 17104, Korea
1세명대학교 보건바이오대학 바이오식품산업학부, 교수
2경희대학교 생명과학대학 한방재료공학과, 교수
* Corresponding Author
1 April 2020. pp. 63-72
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Abstract

Fermented complex from garlic and nine medicinal plants were developed as a natural whitening material. Tyrosinase inhibition activity was determined and four active compounds were isolated. The nutritional components of fermented garlic complex (FGC) were analyzed to confirm the applicability as a functional food material. Tyrosinase inhibitory effect of FGC was 88.6%. Methanol extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc fraction (47 g), which showed the highest yield, active fractions were separated by repeated TLC, silica gel and ODS column chromatography to isolate active compounds. The chemical structures of the isolated compounds were analyzed by NMR and MS spectra. Phenylpropanoid compounds of 2,4,5-trihydroxy-benzenepropanoic acid (1) (1.9 ㎎) and 2,3,5- trihydroxy-benzenepropanoic acid (2) were confirmed. In addition, 2,4-dihydroxy-hydrocinnamic acid (3) (3.3 ㎎) and (+)sesamin (4) (6.1 ㎎) were isolated. These compounds will be useful as index compounds or functional compounds in FGC.

Keywords
2,4-Dihydroxy-hydrocinnamic acid
2,3,5-Trihydroxy-benzenepropanoic acid
2,4,5-Trihydroxy-benzenepropanoic acid
Fermented garlic complex
Tyrosinase inhibition activity
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 33
  • No :2
  • Pages :63-72
  • Received Date : 2020-03-13
  • Revised Date : 2020-03-14
  • Accepted Date : 2020-03-14
  • DOI :https://doi.org/10.7732/kjpr.2020.33.2.063
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