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2018 Vol.31, Issue 6 Preview Page

Research Article

Anemarrhena asphodeloides Extract Inhibits the Mycelial Growth of Magnaporthe oryzae and Controls the Rice Blast Disease
Myoung Ho Joo12
,
Yu Mi Yeo3
,
Pil Son Choi4
,
Jae Hyeok Lee4
,
Kwang-Yeol Yang5
,
Young Jin Lee4*
1Graduate student, Department of Plant Biotechnology, Chonnam National University
2Researcher, Department of Oriental Pharmaceutical Development, The Nambu University
3Researcher, Department of Agricultural Biotechnology, National Institute of Agricultural Science, Rural Development Administration
4Professor, Department of Oriental Pharmaceutical Development, The Nambu University
5Professor, Department of Plant Biotechnology, Chonnam National University
*Corresponding Author
31 December 2018. pp. 695-703
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Abstract

Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a new environment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenic fungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitory effects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showed prominent inhibitory effect of which IC50 was 139.7 ㎍/㎖. Mycelial radii of M. oryzae were measured on PDA medium containing the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fraction showed the impressive inhibitory effect of IC50, 54.12 ㎍/㎖. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almost equivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, the analysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for the verification of cellular mechanism by which the mycelial growth of M. oryzae inhibited.

Keywords
Biopesticide
Control rate
Mycelial inhibition
Phytopathogenic fungi
Plant extract
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :6
  • Pages :695-703
  • Received Date : 2018-11-26
  • Revised Date : 2018-12-18
  • Accepted Date : 2018-12-19
  • DOI :https://doi.org/10.7732/kjpr.2018.31.6.695
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