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2019 Vol.32, Issue 3 Preview Page

Research Article

Antioxidant and Antimelanogenic Effects of Stevia rebaudiana Flower Extract
Gyeongseop So1*
,
Sung Ryul Lee2*
,
Sung Hyeok Kim1
,
Chang Woo Ha1
,
Yuna Park3
,
Sohee Jang4
,
Jong Phil Bak5
,
Hyun Jung Koo6
,
Eun-Hwa Sohn7**
1Graduate Student, Department of Herbal Medicine Resource, Kangwon National University
2Professor, Department of Convergence Biomedical Science, Cardiovascular and Metabolic Disease Center, Inje University
3Master’s Degree Researcher, Department of Herbal Medicine Resource, Kangwon National University
4Undergraduate Student, Department of Herbal Medicine Resource, Kangwon National University
5Post-doc, Department of Herbal Medicine Resource, Kangwon National University
6Professor, Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries
7Professor, Department of Herbal Medicine Resource, Kangwon National University
* First Author
** Corresponding Author
30 June 2019. pp. 220-227
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Abstract

Stevia rebaudiana (Asteraceae), a perennial plant, has been used as a low-calorie sweetener and is being developed as a therapeutic agent for diabetes, hypertension, myocardial diseases, and microbial infections. Despite the common use of its leaves and stem, the bioavailability of the components present in S. rebaudiana flowers, when used as ingredients of cosmetics, has not been well investigated. Herein, we investigated the antioxidative and antimelanogenic effects of an aqueous extract of S. rebaudiana flowers (Stevia-F). Total flavonoid and phenolic content in Stevia-F were determined to be 8.64 ± 0.23 ㎎ of quercetin equivalents/100 g and 631.5 ± 2.01 ㎎ of gallic acid equivalents/100 g, respectively. The IC50 values of Stevia-F for reducing power, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical, hydrogen peroxide, and nitric oxide scavenging activities were 5541.96, 131.39, 466.34, and 10.44 ㎍/mL, respectively. Stevia-F showed inhibitory effects on the tyrosinase (IC50 = 134.74 ㎍/mL) and α-glucosidase (IC50 = 114.81 ㎍/mL) activities. No significant cytotoxicity of Stevia-F was observed in B16F10 cells, treated with up to 100 ㎍/mL of the extract for 24 and 48 h (p > 0.05). Stevia-F (1–100 ㎍/mL) suppressed α-melanocyte stimulating hormone-induced melanin production in B16F10 cells (p < 0.05) and also inhibited the cellular tyrosinase activity (p < 0.05). Overall, our results show that Stevia-F possesses potential for inhibiting tyrosinase and α-glucosidase activities and has significant antioxidant capacity. The antimelanogenic potential of Stevia-F should extend the usage of S. rebaudiana flowers in the development of skin- whitening products.

Keywords
Antioxidant
Antimelanogenic
Flower extract
Stevia rebaudiana
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 32
  • No :3
  • Pages :220-227
  • Received Date : 2019-06-04
  • Revised Date : 2019-06-13
  • Accepted Date : 2019-06-14
  • DOI :https://doi.org/10.7732/kjpr.2019.32.3.220
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