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2021 Vol.34, Issue 3 Preview Page

Research Article

In vitro Immunostimulatory Activity of Bok Choy (Brassica campestris var. chinensis) Sprouts in RAW264.7 Macrophage Cells
Na Gyeong Geum1
,
Joo Ho Yeo2
,
Ju Hyeong Yu3
,
Min Yeong Choi4
,
Jae Won Lee5
,
Jueng Kyu Baek6
,
Jin Boo Jeong7
1Graduate Student, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
2Chief Researcher, Agricultural Corporation E·Farm Corp., Yeongju 36042, Korea
3Researcher, Agricultural Corporation E·Farm Corp., Yeongju 36042, Korea
4Researcher, PINOGEN Co., Ltd, Andong 36746, Korea
5Research Director, Agricultural Corporation E·Farm Corp., Yeongju 36042, Korea
6Post-doc, Institute of Agricultural Science and Technology, Andong National University, Andong 36729, Korea
7Professor, Department of Medicinal Plant Resources, Andong National University, Andong 36729, Korea
*Corresponding Author
31 May 2021. pp. 203-215
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Abstract

Bok choy is one of Brassica vegetables widely consumed worldwide. Brassica vegetables have been reported to exert various pharmacological activities such as antioxidant, anti-cancer and cardioprotective activity. However, studies on immunostimulatory activity of bok choy sprout have not been conducted properly. Thus, in this study, we investigated in vitro immunostimulatory activity of bok choy sprout extract (BCS) using mouse macrophage RAW264.7 cells. Our results showed that BCS increased the production of immunomodulators such as NO, iNOS, IL-1β, IL-6, IL-12, TNF-α and MCP-1, and phagocytic activity in RAW264.7 cells. BCS activated MAPK, NF-κB and PI3K/AKT signaling pathways. However, BCS-mediated production of immunomodulators was dependent on JNK, NF-κB and PI3K/AKT signaling pathways. the mRNA expression of TLR2 were significantly increased by BCS, TLR2 inhibition by anti-TLR2 dramatically suppressed the production of immunomodulators by BCS. In addition, TLR2 inhibition by anti-TLR2 significantly reduced BCS-mediated phosphorylation level of AKT, JNK and NF-κB. From these results, BCS may have immunostimulatory activity via TLR2-MAPK, NF-κB and PI3K/AKT signaling pathways. Therefore, BCS expected to be used as a potential immune-enhancing agent.

Keywords
Bok choy sprout
Brassica campestris var. chinensis
Immunostimulatory activity
Macrophage activation
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  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :3
  • Pages :203-215
  • Received Date : 2021-04-05
  • Revised Date : 2021-05-20
  • Accepted Date : 2021-05-20
  • DOI :https://doi.org/10.7732/kjpr.2021.34.3.203
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