Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor,  and the Antioxidant and Whitening Activity of the Extract

Jong-Du Lee; Ho Bong Hyun; Hyejin Hyeon; Eunbi Jang; Min-Hee Ko; Weon-Jong Yoon; Young Min Ham; Yong-Hwan Jung; Hwon Choi; Eu Gene O; Daeju Oh

doi:10.7732/kjpr.2022.35.4.435

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2022 Vol.35, Issue 4 Preview Page Next Page

Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract 생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가

1 August 2022. pp. 435-444

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Abstract

Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17 ~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 ㎎/L sucrose, and 2 ㎎/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

Keywords

Adventitious root culture

Bioreactor

Bioactive compounds

Endangered plant

Hibiscus hamabo

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Information

Publisher :The Plant Resources Society of Korea
Publisher(Ko) :한국자원식물학회
Journal Title :Korean Journal of Plant Resources
Journal Title(Ko) :한국자원식물학회지
Volume : 35
No :4
Pages :435-444
Received Date : 2022-01-11
Revised Date : 2022-03-28
Accepted Date : 2022-03-30
DOI :https://doi.org/10.7732/kjpr.2022.35.4.435

Korean Journal of Plant Resources ISSN:1226-3591(Print) 2287-8203(Online) 한국자원식물학회지

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