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2021 Vol.34, Issue 2 Preview Page

Research Article

In vitro Regeneration and Genetic Stability Analysis of the Regenerated Green Plants in Japanese Blood Grass (Imperata cylindrica ‘Rubra’)

홍띠 기내 재생과 재생 녹색식물체의 유전적 안정성

In-Jin Kang1
,
Ye-Jin Lee1
,
Chang-Hyu Bae12*
강 인진1
,
이 예진1
,
배 창휴12*
1Graduate School Student, Department of Plant Production Sciences, Graduate School of Sunchon National University, Suncheon 57922, Korea
2Professor, Department of Well-being Bioresources, Sunchon National University, Suncheon 57922, Korea
1순천대학교 대학원 식물생산과학부, 대학원생
2순천대학교 웰빙자원학과, 교수
*Corresponding Author
1 April 2021. pp. 156-165
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Abstract

The in vitro regeneration was established, and the genetic stability among the mother plants (control) and the micropropagated green plants was evaluated using ISSR markers in Imperata cylindrica ‘Rubra’, Poaceae which containing important bioenergy plants. Green shoots were multiply induced from growing point culture via callus on MS medium supplemented with 0.01 mg/L NAA and 2 mg/L BA, and the shoots were proliferated on the MS medium with rooting. Rooted plantlets were transplanted to the pot with 100% survival rate. Using ISSR markers, somaclonal variation was analyzed in eight mother plants (control), ten green-regenerant cultivated at culture room (ReR) and ten green-regenerant cultivated at field condition (ReF). All ISSRs produced a total of 97 bands, and the scorable bands varied from one to seven with an average of 4.4 bands per primer. The polymorphism rate of ReRs and ReFs was 4.1% and 3.1% respectively, showing higher rate than that of control (0%). The genetic similarity matrix (GSM) among all accessions ranged from 0.919 to 1.0 with a mean of 0.972. According to the clustering analysis, ReFs and mother plants were divided into two independent groups. The results indicate that no clear genetic diversity was detected among regenerated plants, and ISSR markers were useful tool for identification of somaclonal variation of regenerants.

Keywords
Genetic similarity matrix (GSM)
Imperata cylindrica
In vitro regeneration
ISSR marker
Somaclonal variation
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 34
  • No :2
  • Pages :156-165
  • Received Date : 2021-01-15
  • Revised Date : 2021-03-03
  • Accepted Date : 2021-03-04
  • DOI :https://doi.org/10.7732/kjpr.2021.34.2.156
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