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2025 Vol.38, Issue 1 Preview Page

Research Article

Autophagy-Inducing Activity of Wild Simulated Ginseng Extract through Increasing p62/SQSTM1 in RAW264.7 Mouse Macrophages

마우스 대식세포 주 RAW264.7 세포에서 산양삼 추출물의 p62/SQSTM1 증가를 통한 자가포식 유도 활성

Jeong Won Choi1†
,
Yurry Um2†
,
Hyeok Jin Choi1
,
So Jung Park1
,
Sang Hun Lee1
,
Jin Hyuk Hwang1
,
Yeong-Bae Yun3
,
Jin Boo Jeong4
최 정원1†
,
엄 유리2†
,
최 혁진1
,
박 소정1
,
이 상훈1
,
황 진혁1
,
윤 영배3
,
정 진부4
1Graduate Student, Department of Forest Science, Andong National University, Andong 36729, Korea
2Researcher, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
3Post-doc, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Korea
4Professor, Department of Forest Science, Andong National University, Andong 36729, Korea
1국립안동대학교 산림과학과, 대학원생
2국립산립과학원 산림약용자원연구소, 연구사
3국립산립과학원 산림약용자원연구소, 박사후 연구원
4국립안동대학교 산림과학과, 교수
*Corresponding Author
†These authors contributed equally to this work.
1 February 2025. pp. 10-17
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Abstract

Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, WSG increased p62/SQSTM1. Inhibition of TLR2 and TLR4 did not block WSG-mediated increase in p62/SQSTM1 level. However, inhibition of PI3K and AMPK blocked WSG-mediated increase of p62/SQSTM1. WSG activated PI3K and AMPK. WSG increased Nrf2 level and the inhibition of PI3K and AMPK reduced WSG-mediated increase of Nrf2. Taken together, it is believed that WSG may induce autophagy through upregulating p62/SQSTM1 via PI3K and AMPK/Nrf2 signaling pathway.

Keywords
Autophagy
Macrophage
Wild simulated ginseng
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 38
  • No :1
  • Pages :10-17
  • Received Date : 2024-12-10
  • Revised Date : 2024-12-18
  • Accepted Date : 2024-12-18
  • DOI :https://doi.org/10.7732/kjpr.2025.38.1.010
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