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2018 Vol.31, Issue 1 Preview Page

Research Article

Study on Molecular Phylogenetics of Korean Arisaema Species Based on Universal DNA Barcodes

범용성 DNA 바코드 분석 기반 한국산 천남성속(Arisaema) 식물의 분자계통학적 연구

Pureum Noh1
,
Kyeongsuk Han1
,
Wook Jin Kim1
,
Sungyu Yang1
,
Goya Choi1
,
Sung Chul Ko2
,
Byeong Cheol Moon1*
노 푸름1
,
한 경숙11
,
김 욱진1
,
양 선규1
,
최 고야1
,
고 성철2
,
문 병철1*
1K-herb Research Center, Korea Institute of Oriental Medicine
2Department of Biological Science and Biotechnology
1한국한의학연구원 K-herb 연구단
2한남대학교 생명나노과학대학 생명시스템과학과
* Corresponding Author
28 February 2018. pp. 37-51
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Abstract

Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, SinArisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.

Keywords
Arisaema
DNA barcode
matK
Molecular phylogenetic analysis
rDNA-ITS
rbcL
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Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 31
  • No :1
  • Pages :37-51
  • Received Date : 2017-10-13
  • Revised Date : 2017-12-04
  • Accepted Date : 2018-01-19
  • DOI :https://doi.org/10.7732/kjpr.2018.31.1.037
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