All Issue

2023 Vol.36, Issue 2

Research Article

Comparative Analysis of Antioxidant, Anti Aging and Phenolic Compounds of Different Solvent Extracts from Saccharina japonica and Costaria costata

추출용매에 따른 쇠미역과 다시마의 항산화, 항노화 활성과 페놀화합물 비교분석

Hyun-Hwa Lee1
,
Jin-Sol Kim2
,
Jun-Han Jeong3
,
Chun Sung Kim4
,
Sook Young Lee5*
이 현화1
,
김 진솔2
,
정 준한3
,
김 춘성4
,
이 숙영5*
1Professor, Department of Biology, Chosun University, Gwangju 61452, Korea
2Researcher, Department of Biology, Chosun University, Gwangju 61452, Korea
3Graduate Student, Department of Biology, Chosun University, Gwangju 61452, Korea
4Professor, Department of Oral Biochemistry, Chosun University, Gwangju 61452, Korea
5Professor, Marine Bio Research Center, Chosun University, Wando 59146, Korea
1조선대학교 생명과학과, 교수
2조선대학교 생명과학과, 연구원
3조선대학교 생명과학과, 대학원생
4조선대학교 구강생화학교실, 교수
5조선대학교 해양생물연구교육센터, 교수
*Corresponding Author
1 April 2023. pp. 107-121
PDF XML Citation
Abstract

This study analyzed the polyphenol, flavonoid contents, antioxidant activity, anti-aging activity and phenol component contents of Saccharina japonica (SJ), Costaria costata (CC) extracts with hot water, 95% methanol, 95% prethanol for investigating possible utilization of SJ and CC extracts. The result revealed that the SJ and CC methanol extracts showed the highest polyphenol and flavonoid contents, 4.63 mg TAN/g, and 4.19 mg QUE/g respectively. Also, the SJ and CC methanol extracts showed higher antioxidant activity than prethanol and hot water extracts, whereas the ABTS radical scavenging activities were the highest in prethanol extracts (IC50 = 15.4, 10.3 ㎍/μL). In anti-aging activity for evaluating the anti-wrinkle activity and skin whitening activity, the CC methanol extracts had high collagenase inhibitory activity (88.3%), and the SJ prethanol extracts showed higher elastase inhibitory activity (19.0%) compared to other extracts. Then the tyrosinase inhibitory activity was significantly higher in the SJ and CC methanol extracts (41.8, 30.3%, respectively), whereas prethanol extracts were the lowest. To identify the phenol component contents of SJ and CC extracts, 4-hydroxybenzoic acid, naringenin, naringin and nicotinic acid were measured using LC-MS/MS. As a result, the phenol contents were the highest in SJ methanol extract (4-hydroxybenzoic acid), SJ and CC prethanol extract (naringin and naringenin) and CC prethanol extract (nicotinic acid). Lastly, the antioxidant activity of SJ and CC showed high correlations with polyphenol and flavonoid contents (R = -0.946~0.883). These results suggest that prethanol or methanol extracts of SJ and CC have higher antioxidant activities, anti-aging activity and the potential to be used as material for health functional food and cosmetics.

Keywords
Antiaging effect
Antioxidant effect
Costaria costata
Phenol component
Saccharina japonica
References
1
Aebi, H. 1984. Catalase in vitro. Methods Enzymol. 105:121-126. 10.1016/S0076-6879(84)05016-36727660
2
Aguilera, J., A. Dummermuth, U. Karsten, R. Schriek and C. Wiencke. 2002. Enzymatic defences against photooxidative stress induced by ultraviolet radiation in arctic marine macroalgae. Polar Biol. 25(6):432-441. 10.1007/s00300-002-0362-2
3
Al-Hazzani, A.A. and A.A. Alshatwi. 2011. Catechin hydrate inhibits proliferation and mediates apoptosis of SiHa human cervical cancer cells. Food. Chem. Toxicol. 49(12):3281-3286. 10.1016/j.fct.2011.09.02321967781
4
Aminina, N., T. Vishnevskaya, E. Karaulova, N. Epur and E. Yakush. 2020a. Prospects for the use of commercial and potentially commercial brown algae of the far eastern seas as a source of polyphenols. Russ. J. Mar. Biol. 46(1):34-41. 10.1134/S1063074020010022
5
Aminina, N.M., E.P. Karaulova, T.I. Vishnevskaya, E.V. Yakush, Y.K. Kim, K.H. Nam and K.T. Son. 2020b. Characteristics of polyphenolic content in brown algae of the pacific coast of russia. Molecules 25(17):3909. 10.3390/molecules2517390932867195PMC7504090
6
Andrade, J.E. and J.R. Burgess. 2007. Effect of the citrus flavanone naringenin on oxidative stress in rats. J. Agr. Food. Chem. 55(6):2142-2148. 10.1021/jf061714h17315884
7
Baek, S.H., H.J. Lee, C.H. Lee, T.J. Nam and S.G. Lee. 2019. Change of fucoxanthin and total antioxidant capacities of Saccharina japonica during the drying process. Korean J. Food Sci. Technol. 51(6):524-530.
8
Bangmei, X. and I.A. Abbott. 1987. Edible seaweeds of china and their place in the chinese diet. Eco. Bot. 41(3):341-353. 10.1007/BF02859049
9
Baweja, P. and D. Sahoo. 2015. Classification of Algae. In Sahoo, D. and J. Seckbach (eds.), The Algae World, Springer, Dordrecht, Netherlands. pp. 31-55. 10.1007/978-94-017-7321-8_2
10
Beauchamp, C. and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44(1):276-287. 10.1016/0003-2697(71)90370-84943714
11
Ben Abdallah Kolsi, R., A. Ben Gara, R. Chaaben, A. El Feki, F. Paolo Patti, L. El Feki and K. Belghith. 2015. Anti-obesity and lipid lowering effects of Cymodocea nodosa sulphated polysaccharide on high cholesterol-fed-rats. Arch. Physiol. Biochem. 121(5):210-217. 10.3109/13813455.2015.110526626599414
12
Bischof, K. and R. Rautenberger. 2012. Seaweed Responses to Environmental Stress: Reactive Oxygen and Antioxidative Strategies. In: Wiencke, C. and K. Bischof (eds.), Springer, Heidelberg, Berlin, Germany, pp. 109-132. 10.1007/978-3-642-28451-9_6
13
Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181(4617):1199-1200. 10.1038/1811199a0
14
Cannell, R.J., S.J. Kellam, A.M. Owsianka and J.M. Walker. 1988. Results of a large scale screen of microalgae for the production of protease inhibitors. J. Med. Plants Res. 54(01):10-14. 10.1055/s-2006-9623193375330
15
Castejón, N., K.A. Thorarinsdottir, R. Einarsdóttir, K. Kristbergsson and G. Marteinsdóttir. 2021. Exploring the potential of icelandic seaweeds extracts produced by aqueous pulsed electric fields-assisted extraction for cosmetic applications. Mar. Drugs. 19(12):662. 10.3390/md1912066234940661PMC8704373
16
Choi, Y.S., J.H. Choi, D.J. Han, H.Y. Kim, H.W. Kim, M.A. Lee, H.J. Chung and C.J. Kim. 2012. Effects of Laminaria japonica on the physico-chemical and sensory characteristics of reduced-fat pork patties. Meat. Sci. 91(1):1-7. 10.1016/j.meatsci.2011.11.01122227100
17
Cornish, M.L. and D.J. Garbary. 2010. Antioxidants from macroalgae: potential applications in human health and nutrition. Algae 25(4):155-171. 10.4490/algae.2010.25.4.155
18
Dang, T.T., M.C. Bowyer, I.A. Van Altena and C.J. Scarlett. 2018. Comparison of chemical profile and antioxidant properties of the brown algae. Int. J. Food Sci. Tech. 53(1):174-181. 10.1111/ijfs.13571
19
Delaney, A., K. Frangoudes and S.A. Ii. 2016. Society and Seaweed: Understanding the Past and Present. Academic Press, MA (USA). pp. 7-40. 10.1016/B978-0-12-802772-1.00002-6PMC5495026
20
Dinh, T.V., P.S. Saravana, H.C. Woo and B.S. Chun. 2018. Ionic liquid-assisted subcritical water enhances the extraction of phenolics from brown seaweed and its antioxidant activity. Sep. Purif. Technol. 196:287-299. 10.1016/j.seppur.2017.06.009
21
FAO. 2020. The State of World Fisheries and Aquaculture 2020 (Sustainability in Action). Rome, Italy. p. 32.
22
Farvin, K.S. and C. Jacobsen. 2013. Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast. Food. Chemistry 138(2-3):1670-1681. 10.1016/j.foodchem.2012.10.07823411297
23
Fatma, C., Ö. Yilmaz, F. Durucan and N.Ş. Özdemir. 2015. Biochemical components of three marine macroalgae (Padina pavonica, Ulva lactuca and Taonia atomaria) from the levantine sea coast of antalya, Turkey. J. Biol. Env. Sci. 6:401-411.
24
Folin, O. and W. Denis. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12(2):239-243. 10.1016/S0021-9258(18)88697-5
25
Ganesan, A.R., U. Tiwari and G. Rajauria. 2019. Seaweed nutraceuticals and their therapeutic role in disease prevention. Food Sci. Hum. Wellness 8(3):252-263. 10.1016/j.fshw.2019.08.001
26
Gehring, W. 2004. Nicotinic acid/niacinamide and the skin. J. Cosmet. Dermatol. 3(2):88-93. 10.1111/j.1473-2130.2004.00115.x17147561
27
Guo, L., A. Tuyama, C. Butkinaree, K. Chung, M.D. O’Donnell, E. Montenont and E.A. Fisher. 2013. Niacin (vitamin B3, nicotinic acid) decreases VLDLA‐polipoprotein B secretion and reduces hepatic and blood lipid concentrations: roles of niacin metabolism and autophagy degradation. FASEB. J. 27(S1):361.1-361.4. 10.1096/fasebj.27.1_supplement.361.4PMC3834778
28
Haijin, M., J. Xiaolu and G. Huashi. 2003. A κ-carrageenan derived oligosaccharide prepared by enzymatic degradation containing anti-tumor activity. J. Appl. Phycol. 15(4):297-303. 10.1023/A:1025103530534
29
Hwang, H.J., S.Y. Lee, S.M. Kim and S.B. Lee. 2011. Fermentation of seaweed sugars by Lactobacillus species and the potential of seaweed as a biomass feedstock. Biotechnol. Bioprocess Eng. 16(6):1231-1239. 10.1007/s12257-011-0278-1
30
Hwang, J.Y., J.S. Jang, D.G. Ryu, K.T. Kim, M.K. Huh and S.H. Eom. 2019. Quality characteristics of the myungran-jeot with Saccharina japonica water extract fermented by lactic acid bacteria. Korean J. Fish. Aquat Sci. 52(3):193-198 (in Korean).
31
Ibraheem, I.B.M., N. Abdel-Raouf, H.M. Mohamed, R. Fassihy and S. Hamed. 2017. Impact of the microbial suppression by using the brown alga Dictyota dichotoma extract. Egypt. J. Bot. 57(7th International Conf.):205-214. 10.21608/ejbo.2017.912.1072
32
Jeong, Y.U. and Y.J. Park. 2018. Studies on Antioxidant, Anti-Inflammation and collagenase inhibitory effects of extracts from plants of the Salix genus. J. Soc. Cosmet. Scientists Korea 44(3):335-341 (in Korean).
33
Kang, S.Y., E. Kim, I. Kang, M. Lee and Y. Lee. 2018. Anti-diabetic effects and anti-inflammatory effects of Laminaria japonica and Hizikia fusiforme in skeletal muscle: in vitro and in vivo model. Nutrients 10(4):491. 10.3390/nu1004049129659527PMC5946276
34
Kim, H.J., S.I. Kim and Y.S. Han. 2008. Effects of sea tangle extract and sea tangle yogurt on constipation relief. Korean J. Food. Cook. Science 24(1):59-67 (in Korean).
35
Kim, J.H., D.S. Lee, C.W. Lim, H.Y. Park and J.H. Park. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. Korean J. Fish. Aquat Sci. 35(2):191-195 (in Korean). 10.5657/kfas.2002.35.2.191
36
Kim, K.A., T.H. Oh and S.H. Chun. 2021. Antioxidative activities and protective effects on alcohol-induced oxidative stress in the human hepatic HepG2 cells of Undaria pinnatifida and Costaria costata extracts. J. Mar. Life Sci. 6(2):66-72 (in Korean).
37
Kim, M. and S. Park. 2019. Antioxidant and anti-bacterial effect of Costaria Costata (C. Agardh) Saunders extracts. Korean J. Community Living Sci. 30(4):509-516 (in Korean). 10.7856/kjcls.2019.30.4.509
38
Kim, M.J., Y.T. Jeong, B.S. Hwang, Y. Hwang, D.W. Jeon and Y.T. Oh. 2022. Anti-melanogenic activities of Ranunculus chinensis Bunge via ERK1/2-mediated MITF downregulation. Korean J. Plant Res. 35(6):704-712 (in Korean).
39
Kim, S.J., G.S. Lee, S.H. Moh, J.B. Park, C.K. Auh, Y.J. Chung, T.K. Ryu and T.K. Lee. 2013. Phenolic contents and antioxidant activities of six edible seaweeds. J. Korea Acad-Ind. Coop. Soc. 14(6):3081-3088 (in Korean). 10.5762/KAIS.2013.14.6.3081
40
Kim, Y.J. and H. Uyama. 2005. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell. Mol. Life. Sci. 62(15):1707-1723. 10.1007/s00018-005-5054-y15968468
41
Kim, Y.J. and J.S. Lee. 2020. Microbiological characteristics of whitening tyrosinase inhibitor-producing wild yeasts, Saccharomyces cerevisiae WJSL0191 and Papiliotrema laurentii ON30 and production. Korean J. Mycol. 48(3):285-296 (in Korean).
42
Kolb, N., L. Vallorani, N. Milanovic and V. Stocchi. 2004. Evaluation of marine algae wakame (Undaria pinnatifida) and kombu (Laminaria digitata japonica) as food supplements. Food. Technol. Biotech. 42(1):57-62.
43
Kumari, S., K. Singh, P. Kushwaha and K.S. Kumar. 2022. Functional and niochemical properties of some economically important edible seaweeds. Curr. Res. Nutr. Food Sci. 10(2):802-816. 10.12944/CRNFSJ.10.2.32
44
Lee, C.H., Y.N. Park and S.G. Lee. 2020. Analysis and comparison of bioactive compounds and total antioxidant capabilities of Korean brown algae. Korean J. Food Sci. Technol. 52(1):54-59 (in Korean).
45
Lee, J.D., H.B. Hyun, H.J. Hyeon, E.B. Jang, M.-H. Ko, W.-J. Yoon, Y. M. Han, Y.-H. Jung, H. Choi, E.G. O and D. Oh. 2021. Mass proliferation of Hibiscus hamabo adventitious root in an air-lift bioreactor and the antioxidant and whitening activity of the extract. Korean J. Plant Res. 35(4):435-444 (in Korean).
46
Liu, X., W. Yuan and X. Meng. 2017. Extraction and quantification of phlorotannins from edible brown algae. J. ASABE. 60(1):265-271. 10.13031/trans.12023
47
Lozano Muñoz, I. and N.F. Díaz. 2020. Minerals in edible seaweed: health benefits and food safety issues. Crit. Rev. Food Sci. 62(6):1592-1607. 10.1080/10408398.2020.184463733203217
48
Machu, L., L. Misurcova, J. Vavra Ambrozova, J. Orsavova, J. Mlcek, J. Sochor and T. Jurikova. 2015. Phenolic content and antioxidant capacity in algal food products. Molecules 20(1):1118-1133. 10.3390/molecules2001111825587787PMC6272319
49
Mekinić, G.I., D. Skroza, V. Šimat, I. Hamed, M. Čagalj and Z. Popović Perković. 2019. Phenolic content of brown algae (Pheophyceae) species: extraction, identification, and quantification. Biomolecules 9(6):244. 10.3390/biom906024431234538PMC6628088
50
Moon, H.J., K.S. Park, M.J. Ku, M.S. Lee, S.H. Jeong, T.I. Imbs, T.N. Zvyagintseva, S.P. Ermakova and Y.H. Lee. 2009. Effect of Costaria costata fucoidan on expression of matrix metalloproteinase-1 promoter, mRNA, and protein. J. Nat. Prod. 72(10):1731-1734. 10.1021/np800797v19807114
51
Moreno, M.I.N., M.I. Isla, A.R. Sampietro and M.A. Vattuone. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71(1-2):109-114. 10.1016/S0378-8741(99)00189-010904153
52
Na, Y.J., D.V. Jeon, S.J. Han, C.A.O. Maranguy, D.S. An, H.K. Cha, J.B. Lee, J.H. Yang, H.W. Lee and H.G. Choi. 2016. Crossed effects of light and temperature on the growth and maturation of gametophytes in Costaria costata and Undaria pinnatifida. Korean J. Fish. Aquat Sci. 49(2):190-197 (in Korean). 10.5657/KFAS.2016.0190
53
Naidoo, L., N. Khoza and N.C. Dlova. 2016. A fairer face, a fairer tomorrow? a review of skin lighteners. Cosmetics 3(3):33. 10.3390/cosmetics3030033
54
Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant. Cell. Physiol. 22(5):867-880.
55
Nowak, A., K. Florkowska, J. Zielonka-Brzezicka, W. Duchnik, A. Muzykiewicz and A. Klimowicz. 2021. The effects of extraction techniques on the antioxidant potential of extracts of different parts of milk thistle (Silybum marianum L.). Acta Sci. Pol. Technol. Aliment. 20(1):37-46. 10.17306/J.AFS.2021.081733449518
56
Pak, W.M., K.B.W.R. Kim, M.J. Kim, J.H. Park, N.Y. Bae, S.H. Park and D.H. Ahn. 2016. Anti-melanogenesis and anti-wrinkle effects of Sargassum micracanthum extracts. Microbiol. Biotechnol. Lett. 44(1):19-25 (in Korean). 10.4014/mbl.1510.10002
57
Park, S.K., J.S. Heo, B.Y. Kim, J.N. Song, G.Y. Lim, H.N. Kim and H.G. Choi. 2011. Comparison on the growth of Costaria costata and Undaria pinnatifida sporophytes in culture and their field populations. Korean J. Fish. Aquat Sci. 44(1):71-77 (in Korean) 10.5657/kfas.2011.44.1.071
58
Ribeiro, I.A., J. Rocha, B. Sepodes, H. Mota-Filipe and M.H. Ribeiro. 2008. Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds. J. Mol. Catal. B Enzym. 52:13-18. 10.1016/j.molcatb.2007.10.011
59
Rushdi, M.I., I.A. Abdel-Rahman, H. Saber, E.Z. Attia and U.R. Abdelmohsen. 2022. The natural products and pharmacological biodiversity of brown algae from the genus Dictyopteris. J. Mex. Chem. Soc. 66(1):154-180. 10.29356/jmcs.v66i1.1639
60
Ryzhik, I.V., М.P. Кlindukh and Е.О. Dobychina. 2021. The B-group vitamins in the red alga Palmaria palmatа (Barents Sea): Composition, seasonal changes and influence of abiotic factors. Algal Res. 59:102473. 10.1016/j.algal.2021.102473
61
Seo, Y.R., S.H. Kim and H.S. Song. 2018. Change in the quality of doenjang with Added Saccharina japonica powder fermented by lactic acid bacteria. Korean J. Fish. Aquat Sci. 51(5):477-490 (in Korean).
62
Shin, H.C., H.J. Hwang, K.J. Kang and B.H. Lee. 2006. An antioxidative and antiinflammatory agent for potential treatment of osteoarthritis from Ecklonia cava. Arch. Pharm Res. 29(2):165-171. 10.1007/BF0297427916526282
63
Shin, S.C., M.W. Ahn, J.S. Lee, Y.S. Kim and K.P. Park. 2013. Extraction of fucoxanthin from Undaria pinnatifida and stability of fucoxanthin. Korean Chem. Eng. Res. 51(1):42-46 (in Korean). 10.9713/kcer.2013.51.1.42
64
Tabakaeva, O. and A. Tabakaev. 2016. Amino acids from potentially commercial far-east brown algae Costaria costata and Undaria pinnatifida. Chem. Nat. Compd. 52(2):376-378. 10.1007/s10600-016-1651-2
65
Tanna, B. and A. Mishra. 2019. Nutraceutical potential of seaweed polysaccharides: structure, bioactivity, safety, and toxicity. Compr. Rev. Food Sci. Food Saf. 18(3):817-831. 10.1111/1541-4337.1244133336929
66
Yagi, A., T. Kanbara and N. Morinobu. 1987. Inhibition of Mushroom-Tyrosinase by Aloe Extract. Planta Med. 53(06):515-517. 10.1055/s-2006-96279817269093
67
Yao, Y., H. Xiang, L. You, C. Cui, D. Sun‐Waterhouse and M. Zhao. 2017. Hypolipidaemic and antioxidant capacities of polysaccharides obtained from Laminaria japonica by different extraction media in diet‐induced mouse model. Int. J. Food Sci. Tech. 52(10):2274-2281. 10.1111/ijfs.13508
68
Youn, J.S., Y.J. Kim, H.J. Na, H.R. Jung, C.K. Song, S.Y. Kang and J.Y. Kim. 2019. Antioxidant activity and contents of leaf extracts obtained from Dendropanax morbifera LEV are dependent on the collecting season and extraction condition. Food Sci. Biotechnol. 28(1):201-207. 10.1007/s10068-018-0352-y30815311PMC6365350
69
Zeng, M., X. Wu, F. Li, W. She, L. Zhou, B. Pi, Z. Xu and X. Huang. 2017. Laminaria japonica polysaccharides effectively inhibited the growth of nasopharyngeal carcinoma cells in vivo and in vitro study. Exp. Toxicol Pathol. 69(7):527-532. 10.1016/j.etp.2017.03.00528501488
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :2
  • Pages :107-121
  • Received Date : 2022-09-14
  • Revised Date : 2023-01-09
  • Accepted Date : 2023-01-26
  • DOI :https://doi.org/10.7732/kjpr.2023.36.2.107
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close