All Issue

2023 Vol.36, Issue 5 Preview Page
Growth of Intestinal Bacteria and Intestinal Inflammation of Sprout Extract from Common Buckwheat and Tartary Buckwheat

일반메밀과 쓴메밀의 새싹 추출물의 장내 유익균 증식 및 염증조절 효능 평가

Su Jeong Kim1†
,
Hwang Bae Sohn2†
,
Jong Won Kim3
,
Sanghyun Lim3
,
Jong Nam Lee4
,
Su Hyoung Park4
,
Jung Hwan Nam1
,
Do Yeon Kim1
,
Ye Jin Lee1
,
Dong Chil Chang5
,
Yul Ho Kim6*
김 수정1†
,
손 황배2†
,
김 종원3
,
임 상현3
,
이 종남4
,
박 수형4
,
남 정환1
,
김 도연1
,
이 예진1
,
장 동칠5
,
김 율호6*
1Researcher, Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
2Researcher, Technology Cooperation Bureau, Rural Development Administration, Jeonju 54875, Korea
3R&D Center, Cell Biotech Co., Ltd., Gimpo 10003, Korea
4Senior Researcher, Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang 25342, Korea
5Senior Researcher, Planning & Coordination Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea
6Senior Researcher, Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
1농촌진흥청 국립식량과학원 고령지농업연구소, 연구사
2농촌진흥청 기술협력국 수출농업지원과, 연구사
3㈜쎌바이오텍 세포공학연구소, 연구원
4농촌진흥청 국립식량과학원 고령지농업연구소, 연구관
5농촌진흥청 국립식량과학원 기획조정과, 연구관
6농촌진흥청 국립식량과학원 재배환경과, 연구관
*Corresponding Author
†These authors contributed equally to this work.
1 October 2023. pp. 455-468
PDF XML Citation
Abstract

We aimed to assess the potential growth-promoting effects of buckwheat sprout on intestinal bacteria and their anti-inflammation effects in a cellular model of intestinal inflammation. The growth of Bifidobacterium longum ssp. infantis BT1 was enhanced with the addition of the sprout extract of tartary buckwheat. Further, in the inflammatory model cells cultured with Raw 264.7 cells were treated with buckwheat sprout including each 10 probiotics before the addition of lipopolysaccharide (LPS) to induce inflammation in Raw 264.7 cells. Buckwheat sprout in both Bifidobacterium longum ssp. infantis BT1 and Lacticaseibacillus paracasei LPC5 significantly reduced the production of NO and PGE2. The above results indicate that buckwheat sprout extract which contains with various physiologically active substances such as rutin, quercetin, and choline is effective in suppressing NO and PGE2 production, which are inflammation-related indicators. The present study suggests that buckwheat sprout could induce positive effects on the intestinal beneficial bacteria and in anti-inflammation.

Keywords
Anti-inflammation
Intestinal bacteria
Prebiotics
Probiotics
Rutin
References
1
Bae, J.Y. 2012. The effect of fermented extracts of Portulaca oleracea against Campylobacter jejuni. Korean J. Food Nutr. 25(2):291-298. 10.9799/ksfan.2012.25.2.291
2
Bruce, S.J., P.A. Guy, S. Rezzi and A.B. Ross. 2010. Quantitative measurement of betaine and free choline in plasma, cereals and cereal products by isotope dilution LC-MS/MS. J. Agric. Food Chem. 58(4):2055-2061. 10.1021/jf903930k20102202
3
Chang, D.R. and J.B. Kim. 2010. A case study on Pulmone healthy life's 'Pulmone vegetable lactic acid bacteria' advertising campaign: creating a new category in a growth market. Korean J. Advertising 21:253-265.
4
Choi, J.Y. 2013. Protective effects of tartary buckwheat from recognition impairment through anti-inflammation and protection from neuronal cell. Department of Food Science and Nutrition, MS Thesis, Pusan National Univ., Korea. pp. 7-90.
5
Chow, J. 2002. Probiotics and prebiotics: a brief overview. J. Ren. Nutr. 12:76-86. 10.1053/jren.2002.3175911953920
6
DuPont, A.W. and H.L. DuPont. 2011. The intestinal microbiota and chronic disorders of the gut. Nat. Rev. Gastroenterol. Hepatol. 8:523-531. 10.1038/nrgastro.2011.13321844910
7
Fooks, L.J. and R.G. Gibson. 2002. In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens, FEMS Microbiol. Ecology 39(1):67-75. 10.1111/j.1574-6941.2002.tb00907.x19709185
8
Ganeshpurkar, A. and A.K. Saluja. 2017. The pharmacological potential of rutin. Saudi Pharm. J. 25:149-164. 10.1016/j.jsps.2016.04.02528344465PMC5355559
9
Gullón, B., T.A. Lú-Chau, M.T. Moreira, J.M. Lema and G. Eibes. 2017. Rutin: a review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability. Trends Food Sci. Technol. 67:220-235. 10.1016/j.tifs.2017.07.008
10
Heselmans, M., G. Reid, L.M. Akkermans, H. Savelkoul, H. Timmerman and F.M. Rombouts. 2005. Gut flora in health and disease: potential role of probiotics. Curr. Issues Intest. Microbiol. 6:1-7.
11
Hwang, E.J., S.Y. Lee, S.J. Kwon, M.H. Park and H.O. Boo. 2006. Antioxidative, antimicrobial and cytotoxic activities of Fagopyrum esculentum Moench extract in germinated seeds. J. Korean Medicinal Crop Sci. 4:1-7.
12
Jeon, A.Y., K.H. Kim, S.J. Kwon, S.K. Roy, S.W. Cho and S.H. Woo. 2015. Effects of LED light conditions on growth and analysis of functional components in buckwheat sprout. Korean J. Crop Sci. 60(3):388-393. 10.7740/kjcs.2015.60.3.388
13
Karimi, O. and A.S. Peria. 2008. Indications and challenges of probiotics, prebiotics, and synbiotics in the management of arthralgias and spondyloarthropathies in inflammatory bowel disease. J. Clin. Gastroenterol. 42:136S-141S. 10.1097/MCG.0b013e318166245518806706
14
Kim, S.J. and Y.H. Kim. 2018. Agricultural Guide Buckwheat. Rural Development Administration, Jeonju, Korea. pp. 7-90.
15
Kim, S.J., H.B. Sohn, B. Kwon, M.S. Park, S.Y. Hong, J.H. Nam, J.T. Suh, J.N. Lee, K.D. Kim, D.C. Chang and Y.H. Kim. 2020. Potential prebiotic effects of yacon extract, a source of fructooligosaccharides, on Bifidobacterium strains. Korean J. Food Sci. Technol. 52(2): 149-155.
16
Kim, S.J., H.B. Sohn, G.H. Kim, Y.Y. Lee, S.Y. Hong, K.D. Kim, J.H. Nam, D.C. Chang, J.T. Suh, B.J. Koo and Y.H. Kim. 2017. Comparison and validation of rutin and quercetin contents according to the extraction method of tartary buckwheat (Fagopyrum tataricum Gaertn.). Korean J. Food Sci. Technol. 49:258-264.
17
Kim, S.J., H.B. Sohn, J.H. Nam, J.N. Lee, J.T. Suh, D.C. Chang and Y.H. Kim. 2022a. Selection and application of pollinating insects to improve seed production of buckwheat in net house. Korean J. Plant Res. 35(1):10-22.
18
Kim, S.J., H.B. Sohn, J.M. Choi, E.J. Cho, J.H. Nam, J.N. Lee, J.T. Suh, D.C. Chang and Y.H. Kim. 2022b. Anti-diabetic effects of common buckwheat and tartary buckwheat in type II diabetes animal model. Korean J. Food Sci. Technol. 54(1):17-27.
19
Kim, S.J., H.B. Sohn, K.T. Lee, J.S. Shin, S. Kim, J.H. Nam, S.Y. Hong, J.T. Suh, D.C. Chang and Y.H. Kim. 2019. Anti-inflammatory effects of seed ethanolic extracts of the common buckwheat and tartary buckwheat are mediated through the suppression of inducible nitric oxide synthase and pro-inflammatory cytokines in LPS-induced Raw 264.7 macrophage cells. Korean J. Food Sci. Technol. 51(6):565- 575.
20
Kim, S.J., O.K. Kwon, S.Y. Hong, J.W. Nam, H.W. Sohn, Y.I. Jin, D.C. Chang and M.J. Cho. 2013. Characteristics and effects of fructooligosaccharides in yacon (Samallanthus sonchifolius). Korean J. Intl. Agri. 25:284-289. 10.12719/KSIA.2013.25.3.284
21
Kwon, C., J.W. Kim, Y.K. Park, S. Kang, M.J. Chung, S.J. Kim and S. Lim. 2023. Bioconversion of rutin in tartary buckwheat by the Korean indigenous probiotics. Microbiol. Biotechnol. Lett. 51(1):1-10. 10.48022/mbl.2302.02004
22
Lee, M.R., K.A. Lee, and S.Y. Lee. 2003. Improving effects of fructooligosaccharides and isomalto-oligosaccharides contained in sponge cakes on the constipation of female college students. Korean J. Food Sci. Technol. 32:621-626. 10.3746/jkfn.2003.32.4.621
23
Marshall, H.G., Y. Pomeranz and G. Chapter. 1982. Buckwheat description, breeding, production and utilization. In Pomeranz, Y. (ed.), Advanced in Cereal and Technology, Vol. V. Am. Ass. Cereal Chem. Incorporated, St. Paul, MN (USA). pp. 157-210.
24
Niu, D., S. An, X. Chen, H. Bi, Q. Zhang, T. Wang, B. Han, H. Zhang and J. Kang. 2020. Corni fructus as a natural resource can treat type 2 diabetes by regulating gut microbiota. Am. J. Chin. Med. 48(6):1385-1407. 10.1142/S0192415X2050068832907359
25
Nuryana, I., A. Andriani, P. Lisdiyanti and Yopi. 2019. Analysis of organic acids produced by lactic acid bacteria. IOP Conf. Ser.: Earth Environ. Sci. 251:012054. 10.1088/1755-1315/251/1/012054
26
Oh, N.S. and M.J. In. 2008. Production of a fermented soymilk using a new strain Leuconostoc mesenteroides Kc51 isolated from Kimchi. J. Korean Soc. Appl. Biol. Chem. 51(1):88-91.
27
Phillips, M.M. 2012. Analytical approaches to determination of total choline in foods and dietary supplements. Anal. Bioanal. Chem. 403:2103-2112. 10.1007/s00216-011-5652-522286077
28
Sung, M.K, S.J. Han, H.J. Seo, S.W. Choi, S.H. Nam and J.I. Chung. 2014. Genotype and environment influence on raffinose and stachyose content of soybean seed. Korean Soc. Crop Sci. 59(3):319-324. 10.7740/kjcs.2014.59.3.319
29
USDA Food Composition Databases (USDA). 2018. Nutrient Lists. USDA National Nutrient Database-Choline USDA. National Nutrient Database for Standard Reference Legacy online.
30
Yun, J.Y, S.Y. Choi, P.J. Park, H.G. Chung, H.M. Shin, K.H. Suk and B.O. Lim. 2008. Extracts of Artemisia princeps Pampanini inhibit lipopolysaccharide-induced nitric oxide, cyclooxygenase-2, prostaglandin E2, and tumor necrosis factor-α, production from murine macrophage Raw 264.7 Cells. Kor. J. Medi. Crop. Sci. 16:326-331.
31
Zeisel, S.H. and J.K. Blusztajn. 1994. Choline and human nutrition. Annu. Rev. Nutr. 14:269-296. 10.1146/annurev.nu.14.070194.0014137946521
Information
  • Publisher :The Plant Resources Society of Korea
  • Publisher(Ko) :한국자원식물학회
  • Journal Title :Korean Journal of Plant Resources
  • Journal Title(Ko) :한국자원식물학회지
  • Volume : 36
  • No :5
  • Pages :455-468
  • Received Date : 2023-09-06
  • Accepted Date : 2023-09-18
  • DOI :https://doi.org/10.7732/kjpr.2023.36.5.455
Journal Informaiton Korean Journal of Plant Resources Korean Journal of Plant Resources
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • ccl
Journal Informaiton Journal Informaiton - close